HOME >> BIOLOGY >> NEWS
Protein vaccine fully protects mice from lethal aerosol challenge with ricin toxin

rty of RTA, increase protein stability, and maintain its ability to elicit a protective immune response.

In the August online issue of Protein Engineering, Design and Selection, Olson and his team--John H. Carra, Virginia Roxas-Duncan, Robert W. Wannemacher, Leonard A. Smith, and Charles B. Millard--describe using a combination of molecular modeling and protein engineering to design the new vaccine. The team started with an extensive computer-aided analysis of the toxin structure, using a three-dimensional model provided by colleagues at the University of Texas, Austin.

"We compared ricin with other proteins of the same family," Olson explained. "We tried to figure out where the protein molecules are diverging within the family--to see what changes were made by nature so we could make the changes we needed to make."

To improve upon its stability--in effect, to make it go against its natural tendencies--Olson and his team had to change the structure of the RTA molecule. Once they had developed the necessary genetic sequences, they handed them off to Smith and others at USAMRIID for protein engineering.

"We went straight from the computer to molecular biology," explained Smith. "We had to clone and purify the proteins, and test them in animals for toxicity and protection."

RTA 1-33/44-198, the most promising vaccine candidate, was tested in three groups of ten mice each. One group received the purified protein alone; a second group received the protein plus an adjuvant called Alhydrogel; and a third group served as the control, receiving an injection of saline solution instead of the vaccine.

Purified RTA 1-33/44-198 protected 10 out of 10 mice from a whole-body aerosol challenge with lethal doses of ricin. The survival rate was the same with or without the adjuvant. All 10 animals in the unvaccinated control group died.

"Molecular modeling and protein engineering represent a sig
'"/>

Contact: Caree Vander Linden
Caree.Vander-Linden@amedd.army.mil
301-619-2285
US Army Medical Research Institute of Infectious Diseases
4-Aug-2004


Page: 1 2 3

Related biology news :

1. Protein is key to fatal disorder and normal cell function
2. Protein is key for digestive function of the pancreas
3. Proteins show promise for mosquito control
4. Protein involved in childhood disorder linked to cancer
5. Protein fishing in America: The movie
6. Protein key to trafficking in nerve terminals
7. Protein controls acid in cells by direct detection of volume changes, study finds
8. Protein believed to control formation of memory identified by Scripps & UCSD scientists
9. Protein stops blood-vessel growth, holds promise as cancer therapy
10. Proteins transform DNA into molecular velcro
11. Protein engineered to detect nerve gas

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:12/27/2018)... (PRWEB) , ... December 27, 2018 , ... ... 60 cervical discs for implantation at its new Boston facility, and is now ... have additional inventory to immediately expand market share against inferior ball and socket ...
(Date:12/20/2018)... PARK, N.C. (PRWEB) , ... December 20, 2018 ... ... been enhanced through upgraded processes and the completion of a new usability/observation room. ... with the goal of ensuring medical devices and drug delivery devices/combination products are ...
(Date:12/18/2018)... PHOENIX (PRWEB) , ... December 18, 2018 , ... ... Cell, has effectively incorporated artificial intelligence into client marketing. The technology has been ... six years, R3 Stem Cell has been working with clients nationwide on patient ...
Breaking Biology News(10 mins):
(Date:1/8/2019)... ... January 07, 2019 , ... Today ... Noninvasix. This represents the second investment AngelMD has made into Noninvasix in the ... is developing a patient monitor to directly, accurately, and noninvasively measure brain oxygenation ...
(Date:1/4/2019)... ... January 02, 2019 , ... ... and cancer cells in blood, has been named as a partner in Medigen ... company developing cell therapies for cancer treatment, will use CellMax Life’s circulating tumor ...
(Date:12/18/2018)... ... December 17, 2018 , ... ABCT ... emerging biosciences ventures invited to participate in the annual program. Twelve ventures will ... plans and a professional network. All startups receive an entrepreneur coach, access to ...
(Date:12/13/2018)... , ... December 12, 2018 , ... ... life science software solutions, announces the Limfinity® ML (Machine Learning) addition for its ... machine learning models within Limfinity® solutions. In the module, trained models make predictions ...
Breaking Biology Technology:
Cached News: