Chapel Hill -- University of North Carolina at Chapel Hill scientists have discovered the human version of a gene and gene product that is an essential component of the tiny hair-like whips called cilia that cleanse the lungs. They did it by applying what is known about comparable structures in single-celled organisms that enable the tiny creatures to swim around in pond water.
Such novel work will help other researchers trying to understand and combat chronic lung diseases, the UNC-CH scientists said.
The team used knowledge developed by cell biologists who have studied the cilia of sea urchins and single-celled animals for the past 40 years. To pinpoint the human gene, they combined that knowledge with information generated by international efforts to sequence the human genome -- all genes in the body.
"It turns out that these structures are highly conserved, or repeated, in nature such that the cilia on a one-celled animal in a pond are very, very similar to the respiratory cilia in human airways," said Dr. William Reed, research assistant professor of pediatrics at the UNC-CH School of Medicine. "We relied on that high degree of similarity in structure and protein sequence to pull out a human relative of the animal gene."
The gene produces a large protein essential for generating forces that power the whip-like movement of cilia, Reed said. Besides identifying it, they also showed that the gene is "turned on" in cells as they begin growing cilia.
About 80 percent of epithelial cells in healthy human airways are ciliated, he said. They sweep mucous and trapped particles and disease-causing organisms into the throat where they are usually swallowed and eliminated.
Ciliated cells are injured and lost when flu viruses infect the lungs, for example. This reduces the ability of the lung to cleanse itself. Normal recovery involves regeneration of the ciliated cells by a repair process that is poorly understood.
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Contact: David Williamson
david_williamson@unc.edu
919-962-8596
University of North Carolina at Chapel Hill
8-Jan-2001