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Researchers identify protein that regulates killer cells

Researchers at the University of Toronto and Mount Sinai Hospital have identified a protein that plays a critical role in the regulation of "natural killers cells" in the immune system's battle against foreign and diseased cells.

"Our research is a small part of the larger problem of how viruses and diseased cells ravage the body and circumvent our immune system," says Kathleen Binns, a U of T doctoral student in medical genetics and microbiology and an author on a paper in the June 20 issue of Science.

Using mass spectrometry, Binns, who does research in the Samuel Lunenfeld Research Institute at Mount Sinai and MDS Sciex, sequenced and identified a mystery protein from co-researchers at the Swiss Federal Institute of Technology in Switzerland.

Once identified, the protein (SSPase) was sent back to the Swiss researchers where they cloned its gene and sequenced its DNA. That gene, they discovered, is a key component involved in regulation of "natural killer cells" - cells produced by the body's immune system that attack foreign or mutated cells like caused by viruses or cancer.

"This research gives us a better understanding of how the immune system works. As a result, we have a better understanding of how viruses and cancer try to get around this process. One day, we will hopefully be able to develop treatments and therapies to counter these rogue cells," says Binns.

A group of genes called the major histocompatibility complex I (MHC-I) are a natural part of the immune system and present in most cells in the body, explains Binns. Acting like an information relay, the MHC-I molecules retrieve bits and pieces of the proteins from inside the cell and display them on the cell surface. "MHC complexes essentially give a read out of what's inside the cell," she says.

T-cells, one of the main components of the immune system, "examine" the protein fragments on the cell surface and if they recognize them, the T-cells move on
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Contact: Janet Wong
jf.wong@utoronto.ca
416-978-5949
University of Toronto
20-Jun-2002


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