Researchers make key genome public on the Internet

Researchers at the University of Washington School of Medicine, DuPont and the University of Campinas in Brazil, with partial funding from the National Science Foundation, have sequenced the genome of an important organism, Agrobacterium, and made it freely available on the Internet. This information is available at http://www.agrobacterium.org.

Agrobacterium has the unique property of inserting small pieces of genetic material into a plant, animal or fungal cell that it colonizes. The bacterium has had great utility in molecular studies of botany, and has enabled scientists to study the effects of plant genes on various properties of plants such as growth rate, maturation, flower color and yield.

The organism also serves as a useful tool to study a number of infectious human diseases in which the inciting organism injects material into a cell.

On a practical side, Agrobacterium is a basic tool for genetic engineering of foodstuffs to produce crops that are more nutritious, less allergenic and disease-, insect-, salt- and cold-resistant, and a whole host of other promising traits.

The sequencing of the bacterial genome will provide insights into the unique properties of this organism, which enable it to undertake the genetic engineering of its host cells. This work is the culmination of almost two decades of work at the University of Washington by a group of microbiologists including Dr. Eugene Nester, Dr. Milton Gordon and Dr. Mary-Dell Chilton. The scale of the project was so large that the team included Dr. Maynard Olson, professor of medicine and genetics, and his researchers at the University of Washington Genome Center; researchers from DuPont and its subsidiary, Pioneer, who have a large sequencing facility; and the Brazil group, who had previously determined the sequence of another organism important to agriculture.

The genome of Agrobacterium is complex, containing a total of more than

Contact: Walter Neary
University of Washington

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