Researchers pave the way to protein therapy in humans

St. Louis, Sept. 3, 1999--For decades, pharmaceutical companies have struggled to overcome the molecular equivalent of the Great Wall of China: the outer membrane of cells, which prevents all but the tiniest of proteins from entering. Now researchers have slipped a protein that's more than 200 times larger than the average drug into the cells of living mice and shown that the protein functions.

"For the very first time, we've introduced a large, biologically active protein into every cell of the body-- including cells in the brain that are normally protected by the blood-brain barrier," says Steven F. Dowdy, Ph.D., who led the research team at Washington University School of Medicine in St. Louis. The group published its results in today's issue of Science.

Dowdy is an assistant investigator of the Howard Hughes Medical Institute and an assistant professor of pathology and medicine. Steven R. Schwarze, Ph.D., a postdoctoral fellow in his laboratory, was lead author of the paper.

Getting full-sized, therapeutic proteins into cells would be advantageous because smaller drugs tend to interact with unintended targets. Larger proteins fit only onto the molecules for which they were designed, so they could be given in substantially lower doses, resulting in fewer side effects.

Dowdy led a previous research team that used test-tube experiments to smuggle an enzyme into HIV-infected cells. The results, reported in Nature Medicine last December and January focused on a human enzyme that makes cells self-destruct. The enzyme was modified to include a string of 11 amino acids that served as a passport for crossing a cell's outer membrane. But the researchers needed to prove that large proteins could slip into cells in model animals before considering human applications.

In the Science study, Dowdy and fellow investigators first attached a molecular passport known as a protein transduction domain (PTD) to

Contact: Barbra Rodriguez
Washington University School of Medicine

Page: 1 2 3

Related biology news :

1. Researchers determine genetic cause of Timothy syndrome
2. Researchers find color sensitive atomic switch in bacteria
3. Researchers identify protein promoting vascular tumor growth
4. Researchers devise potent new tools to curb ivory poaching
5. Researchers create nanotubes that change colors, form nanocarpet and kill bacteria
6. Researchers ID chlorophyll-regulating gene
7. Researchers develop fast track way to discover how cells are regulated
8. Researchers identify distinctive signature for metastatic prostate cancer
9. Researchers report new gene test for isolated cleft lip and palate
10. Researchers discover why mutant gene causes colon cancer
11. Researchers identify the genomes controlling elements

Post Your Comments:

(Date:5/28/2020)... ... May 28, 2020 , ... ... enzyme solution for adjunct liquefaction: AMYLEX® 6T. The innovative enzyme enables numerous ... into new segments with locally-sourced raw materials, improve productivity, maximize yield, ensure ...
(Date:5/25/2020)... United Kingdom (PRWEB) , ... May 22, 2020 , ... ... (assay) for the detection of SARS-CoV-2, the virus that causes COVID-19. , ... to on-demand analysis of smaller sample numbers, to very large numbers of samples. ...
(Date:5/21/2020)... ... 20, 2020 , ... Catalent, the leading global provider of ... therapies, and consumer health products, today announced that Jean-François Chaubard, Senior Director, Open ... 2020 Paris Virtual conference, which takes place on 28 – 29 May, 2020. ...
Breaking Biology News(10 mins):
(Date:4/22/2020)... ... April 21, 2020 , ... ... method for determination of FXIII. , Aniara Diagnostica, a multi-national distributor of diagnostic ... meeting today’s highest standards for product quality and cost-efficient delivery. , ...
(Date:4/7/2020)... Calif. (PRWEB) , ... April 06, 2020 , ... ... their future children, reached 200,000 SneakPeek Early Gender DNA Tests sold in ... SneakPeek was launched as an ecommerce product, where a mom could order her ...
(Date:4/1/2020)... ... March 30, 2020 , ... ... Reliant Immune Diagnostics (RID), formed earlier this year to bring telehealth to various ... in-home tests for COVID-19. Company officials are currently?awaiting an Emergency Use Authorization (EUA) ...
(Date:4/1/2020)... ... 2020 , ... Bedoukian Research, Inc. (BRI) and ... Agreement that builds on each company’s expertise in developing efficient routes to synthesize ... of high-quality insect pheromones as well as flavors and fragrances. P2 has developed ...
Breaking Biology Technology:
Cached News: