HOME >> BIOLOGY >> NEWS
Researchers view protein's structural changes in real time

HOUSTON Dramatic structural changes that take place inside a protein in less than a billionth of a second have been filmed in high resolution with X-ray crystallography, enabling scientists to view them like a movie.

The ability to watch a protein functioning on the inside may lead to a better understanding of how it works, enabling scientists to engineer improved proteins for blood substitutes and improved treatments for genetic diseases.

Researchers at the National Institutes of Health (NIH), Rice University and the European Synchrotron Radiation Facility (ESRF) in France collaborated on the study, which is published in the June 20 issue of the journal Science.

Philip Anfinrud, senior biomedical research scientist in the Laboratory of Chemical Physics at the NIH's National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK), headed the study of the protein myoglobin, which provides oxygen to muscles when they contract.

The scientists aimed a laser pulse at the protein for a trillionth of a second to break the chemical bond that links carbon monoxide to the protein. Noting the time when this reaction began, the scientists then used X-ray crystallography to take snapshots of the protein's structure, starting at one 10-billionth of a second. By stitching together the series of still images, the researchers created a moving picture of the changes taking place within the protein.

"The movie shows quite clearly the structural changes that contribute to the efficient expulsion of the toxic carbon monoxide from the protein," Anfinrud said.

For the study, the researchers used a mutant of myoglobin developed in the lab of John Olson at Rice, because in this variant of the protein, the rate at which carbon monoxide is swept away from the binding site is sped up by 1,000 times. Carbon monoxide, a toxic gas molecule, was used instead of oxygen because the protein-carbon monoxide complex is more stable and can endure
'"/>

Contact: B.J. Almond
balmond@rice.edu
713-348-6770
Rice University
19-Jun-2003


Page: 1 2

Related biology news :

1. Researchers determine genetic cause of Timothy syndrome
2. Researchers find color sensitive atomic switch in bacteria
3. Researchers identify protein promoting vascular tumor growth
4. Researchers devise potent new tools to curb ivory poaching
5. Researchers create nanotubes that change colors, form nanocarpet and kill bacteria
6. Researchers ID chlorophyll-regulating gene
7. Researchers develop fast track way to discover how cells are regulated
8. Researchers identify distinctive signature for metastatic prostate cancer
9. Researchers report new gene test for isolated cleft lip and palate
10. Researchers discover why mutant gene causes colon cancer
11. Researchers identify the genomes controlling elements

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/1/2020)... ... 2020 , ... Cure Glioblastoma, a registered 501(c)(3) nonprofit research ... adult brain cancer—announced today the appointments of its first Senior Fellows: John Boockvar, ... organization’s initiatives and overall vision. , "Senior Fellows are more than advisors; they ...
(Date:6/28/2020)... ... ... In an upcoming episode scheduled for the fall of 2020, Advancements with Ted ... local listings for more info. , Today, the majority of testing is performed at ... the country. Results are then available several days later. Now, in an effort to ...
(Date:6/23/2020)... ... June 23, 2020 , ... ... services, today announced that the company has received ISO9001:2015 certification for the ... and research industries. The decision to pursue ISO9001 accreditation demonstrates Kemp’s commitment ...
Breaking Biology News(10 mins):
(Date:8/7/2020)... ... August 06, 2020 , ... VGXI, ... it has closed on the purchase of greenfield for a new, expanded manufacturing ... includes over 21 acres in the initial acquisition, with an option to purchase ...
(Date:7/31/2020)... ... July 29, 2020 , ... R3 Stem Cell International is now offering ... 50 million stem cells total, patients may choose which extremities they would like treated. ... joints (BMC Musculoskelet Disord. 2016). At R3 International, umbilical cord tissue is obtained from ...
(Date:7/31/2020)... ... July 29, 2020 , ... ... combine up to three different materials for use with CRM, neurostimulation, vascular, and ... three materials to meet specific design requirements, performance, and cost criteria; especially where ...
(Date:7/31/2020)... ... July 29, 2020 , ... ... top radiation centers in 16 countries, has reached its 20th anniversary of worldwide ... in routine use at top universities including University of Pennsylvania, University of Michigan, ...
Breaking Biology Technology:
Cached News: