HOME >> BIOLOGY >> NEWS
Salt controls size of DNA structures, could improve gene therapy

Researchers have found they can control the size of densely packed DNA structures by changing the salt concentration in solutions containing DNA. The finding could improve the efficiency of gene delivery for medical treatment and disease prevention.

Scientists are seeking to understand the natural mechanism of DNA condensation into nanostructures in particular, toroids, which look like tightly wound garden hoses. Densely packed DNA is nature's efficient way of transporting genetic information, done particularly well by sperm cells and viruses.

Researchers want to mimic this process to improve DNA delivery for gene therapy and DNA-based vaccines, but they face many challenges in the laboratory where DNA in solution typically exists in an extended, rather than condensed state. Scientists have been able to cause DNA to condense into toroids by adding positively charged molecules to samples, but they have had difficulty finding the right molecules to achieve consistent, optimal toroid sizes of less than 50 nanometers.

However, scientists at Georgia Institute of Technology have made a significant advance in controlling the size of DNA toroids. In the July 18, 2003 online issue of the journal Proceedings of the National Academy of Sciences (PNAS), they report that reducing salt concentrations below normal laboratory solution levels shrinks both the diameter and thickness of DNA toroids. This finding resulted from a combined investigation of how static DNA loops and solution conditions might be used to control toroid dimensions.

"But even without static loops present, DNA produces smaller toroids if you reduce the salt concentration," said Nicholas Hud, an associate professor of biochemistry who is leading the study funded by the National Institutes of Health. "We found a systematic relationship between reducing salt and reducing toroid size. It is surprising that such a study was not previously done because salt concentration is such a fundament
'"/>

Contact: Jane Sanders
jane.sanders@edi.gatech.edu
404-894-2214
Georgia Institute of Technology Research News
29-Jul-2003


Page: 1 2 3

Related biology news :

1. Protein controls acid in cells by direct detection of volume changes, study finds
2. Biologists discover nerve activity, not just genetics controls kinds of neurotransmitters produced
3. Newly discovered gene controls levels of bad cholesterol in mice
4. Researchers find a protein that controls cell growth
5. Structure solved by Scripps scientists shows one way that body controls gene expression
6. New findings implicate cell size controls in a variety of diseases
7. Gene controls age at onset of Alzheimers and Parkinsons diseases
8. Transplantation of embryonic pancreatic tissue controls Type 1 diabetes in rats
9. DNA throttle controls molecular machine
10. Modifier gene controls severity of neurological disease in mice
11. Single gene controls leaf form

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/19/2020)... ... June 18, 2020 , ... Join experts from Envigo ... in a live webinar on Wednesday, July 8, 2020 at 11am EDT ... we genetically engineer new models. However, while modifying the genome may be more straightforward, ...
(Date:6/11/2020)... , ... June 09, 2020 , ... Superior Controls, ... industrial automation and IT solutions for the life sciences industry, is pleased to announce ... Development working out of the company’s Seabrook, New Hampshire office. In his new ...
(Date:6/5/2020)... HOUSTON (PRWEB) , ... June 04, 2020 , ... Greffex, ... Bush to its Board of Directors. , “Neil understands the need for a global ... also President and CEO of Greffex. Mr. Bush will serve as an independent, director ...
Breaking Biology News(10 mins):
(Date:6/11/2020)... (PRWEB) , ... June 09, 2020 , ... ... of Phase I of a Small Business Innovation Research (SBIR) program funded by ... 21st Century: Rapid Toxin Detection in the Field”. The project’s goal was to ...
(Date:6/2/2020)... ... June 01, 2020 , ... As part ... positive change in the world, enterprise security solutions provider bitsIO donated time to ... working from home during self-quarantines and shelter-in-place orders, companies are increasingly concerned about ...
(Date:5/28/2020)... (PRWEB) , ... May 28, 2020 , ... The ... and security leaders as the 2020 winners of the Women in Biometrics Awards ... sponsors IDEMIA , Biometric Update and the SIA Women in ...
(Date:5/21/2020)... , ... May 19, 2020 , ... Eaton Square is ... addition of Popper and Company, a biotechnology and medical technology-focused strategy and M&A advisory ... grow in the United States, linking US and Asia Pacific healthcare innovators with capital ...
Breaking Biology Technology:
Cached News: