HOME >> BIOLOGY >> NEWS
Scientists develop new cloning technique that dramatically shortens the search for genes

A single strand of plant or animal DNA may contain tens of thousands of genes, each programmed to produce a specific protein essential for the growth or survival of the organism. The challenge for geneticists is to isolate individual genes and determine their function a painstaking process often requiring years of laboratory trial and error.

Now an international research team has discovered a technique that dramatically streamlines this process for certain kinds of genes. Developed by scientists at Stanford University and Britain's John Innes Centre, the new procedure could enable scientists to identify specific genes in a matter of months, not years. The technique, known as transcript-based cloning, is described in the March 30 edition of the Proceedings of the National Academy of Sciences (PNAS).

"We believe that this method represents a significant breakthrough in gene cloning," wrote the authors of the PNAS study.

"The greatest impact of this technology is likely to be on plants with large and complex genomes, including most crop species," added Sharon R. Long, the William C. Steere, Jr.Pfizer Inc. Professor in Biological Sciences at Stanford and co-author of the study. Long, who also serves as dean of Stanford's School of Humanities and Sciences, is an authority on bacterial and plant molecular biology. She and her colleagues used the new cloning technique to isolate and identify a gene in the DNA of Medicago truncatula, or barrel medic a member of the legume family that is closely related to alfalfa, beans and peas.

"Over the course of six months, we completed what took another group several years to complete, and we identified a pretty cool gene to boot," said Stanford postdoctoral fellow Raka M. Mitra, lead author of the PNAS study. "We think this technology will be applicable to other species and hope that it increases the pace of biological research on the whole."

Reverse genetics

Medicago DNA contai
'"/>

Contact: Mark Shwartz
mshwartz@stanford.edu
650-723-9296
Stanford University
30-Mar-2004


Page: 1 2 3 4 5

Related biology news :

1. Scientists to prototype cyberinfrastructure for research and education access to ocean observatories
2. Scientists sequence genome of kind of organism central to biospheres carbon cycle
3. Scientists find nanowires capable of detecting individual viruses
4. Scientists discover potential new way to control drug-resistant bacteria
5. Scientists explore genome of methane-breathing microbe
6. Scientists decipher genetic code of biothreat pathogen
7. Stuck on you: Scientists lay bare secrets of bacterial attachment proteins
8. Scientists discover proteins involved in spread of HIV-1 infection
9. Scientists fear new Ebola outbreak may explain sudden gorilla disappearance
10. Scientists reinvent DNA as template to produce organic molecules
11. Scientists visualise cellular handmaiden that restores shape to proteins

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/13/2019)... ... June 13, 2019 , ... Global Clinical and Ancillary ... end-to-end clinical and ancillary supply chain management needs of large- and middle-market pharmaceutical, ... today announced it will sponsor a summer education program offered by the Young ...
(Date:6/6/2019)... ... June 05, 2019 , ... ... opening of a new network station and service center in Mexico City, Mexico. ... in the world — with more than $1.75 billion in pharmaceutical exports since ...
(Date:6/4/2019)... ... June 04, 2019 , ... Harper was three years ... X-rays revealed severe elbow dysplasia, which caused painful osteoarthritis in the joints. She ... Her parents were heartbroken. , The surgical specialist, Dr. Christopher Eich of Southern ...
Breaking Biology News(10 mins):
(Date:5/31/2019)... Redwood City, California (PRWEB) , ... ... ... received allowance of our patent applications from the USPTO providing proprietary interest ... SYSTEM AND METHOD FOR SLEEP DISORDER DIAGNOSIS AND TREATMENT… extends Somnology’s IP ...
(Date:5/21/2019)... ... May 21, 2019 , ... ... today officially opened the inaugural Africa Brewing Conference dedicated to sharing brewing knowledge, ... Africa. The event, which is supported through a partnership with “Ethiopia Invest”, will ...
(Date:5/8/2019)... ... ... at 16.2 hands, Atlas is on the large side for a Quarter Horse. He ... other activities. When loping around a corner in late 2017, Atlas tripped and partially ... only being able to walk. Fortunately Atlas’ owner, Kelly, sought the opinion of her ...
(Date:5/2/2019)... ... 2019 , ... The centuries old practice of brewing beer thrives today across ... and even home brewing clubs scattered across the nation. , But proper beer ... can produce a superb product. Students at South Dakota School of Mines & ...
Breaking Biology Technology:
Cached News: