HOME >> BIOLOGY >> NEWS
Scientists develop new cloning technique that dramatically shortens the search for genes

A single strand of plant or animal DNA may contain tens of thousands of genes, each programmed to produce a specific protein essential for the growth or survival of the organism. The challenge for geneticists is to isolate individual genes and determine their function a painstaking process often requiring years of laboratory trial and error.

Now an international research team has discovered a technique that dramatically streamlines this process for certain kinds of genes. Developed by scientists at Stanford University and Britain's John Innes Centre, the new procedure could enable scientists to identify specific genes in a matter of months, not years. The technique, known as transcript-based cloning, is described in the March 30 edition of the Proceedings of the National Academy of Sciences (PNAS).

"We believe that this method represents a significant breakthrough in gene cloning," wrote the authors of the PNAS study.

"The greatest impact of this technology is likely to be on plants with large and complex genomes, including most crop species," added Sharon R. Long, the William C. Steere, Jr.Pfizer Inc. Professor in Biological Sciences at Stanford and co-author of the study. Long, who also serves as dean of Stanford's School of Humanities and Sciences, is an authority on bacterial and plant molecular biology. She and her colleagues used the new cloning technique to isolate and identify a gene in the DNA of Medicago truncatula, or barrel medic a member of the legume family that is closely related to alfalfa, beans and peas.

"Over the course of six months, we completed what took another group several years to complete, and we identified a pretty cool gene to boot," said Stanford postdoctoral fellow Raka M. Mitra, lead author of the PNAS study. "We think this technology will be applicable to other species and hope that it increases the pace of biological research on the whole."

Reverse genetics

Medicago DNA contai
'"/>

Contact: Mark Shwartz
mshwartz@stanford.edu
650-723-9296
Stanford University
30-Mar-2004


Page: 1 2 3 4 5

Related biology news :

1. Scientists to prototype cyberinfrastructure for research and education access to ocean observatories
2. Scientists sequence genome of kind of organism central to biospheres carbon cycle
3. Scientists find nanowires capable of detecting individual viruses
4. Scientists discover potential new way to control drug-resistant bacteria
5. Scientists explore genome of methane-breathing microbe
6. Scientists decipher genetic code of biothreat pathogen
7. Stuck on you: Scientists lay bare secrets of bacterial attachment proteins
8. Scientists discover proteins involved in spread of HIV-1 infection
9. Scientists fear new Ebola outbreak may explain sudden gorilla disappearance
10. Scientists reinvent DNA as template to produce organic molecules
11. Scientists visualise cellular handmaiden that restores shape to proteins

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/26/2018)... ... 25, 2018 , ... Maire Mayne, an evidence-based energy healer, ... to reduce inflammation and autoimmune disorders and improve overall immune response. , The ... The following data was reported:,     Over 180% increase in ...
(Date:8/23/2018)... ... August 21, 2018 , ... CRISPR-Cas9 gene editing strategies have ... for the robust functional interrogation of complex biological processes. Gene editing in primary ... the study of the autoimmunity, the understanding of host-pathogen dynamics that drive HIV ...
(Date:8/23/2018)... ... ... Lajollacooks4u is proud to have hosted a variety of groups this year – both new ... Qualcomm, Hewlett Packard, Sequenom and more, have all visited the La Jolla-based venue time and ... the reason clientele return is due to Lajollacooks4u’s signature team-building format, a way for teams ...
Breaking Biology News(10 mins):
(Date:8/31/2018)... ... 2018 , ... A recent expedition led by Dr. Blair ... of Industrial Science, the University of Tokyo, demonstrated how the use of autonomous ... of hard to reach deep sea ecosystems, like intermittently active methane seeps. Thanks ...
(Date:8/29/2018)... ... August 28, 2018 , ... ... announced today the successful completion of a $5.5 million funding round managed by ... Investment Fund, Commenda Capital, and Ellipsis Ventures. , “Our investment in Nanovis is ...
(Date:8/29/2018)... ... August 28, 2018 , ... Inc. Magazine has ... been ranked in 1138th place on the 27th annual Inc. 5000, the most ... represents the most successful companies within the American economy’s most dynamic segment – ...
(Date:8/29/2018)... YORBA LINDA, Calif. (PRWEB) , ... August 28, 2018 , ... ... engineers, and scientists from around the world, is proud to announce the launch of ... events LabRoots offers for a chance to win one of LabRoots most popular shirts. ...
Breaking Biology Technology:
Cached News: