HOME >> BIOLOGY >> NEWS
Scientists develop new cloning technique that dramatically shortens the search for genes

A single strand of plant or animal DNA may contain tens of thousands of genes, each programmed to produce a specific protein essential for the growth or survival of the organism. The challenge for geneticists is to isolate individual genes and determine their function a painstaking process often requiring years of laboratory trial and error.

Now an international research team has discovered a technique that dramatically streamlines this process for certain kinds of genes. Developed by scientists at Stanford University and Britain's John Innes Centre, the new procedure could enable scientists to identify specific genes in a matter of months, not years. The technique, known as transcript-based cloning, is described in the March 30 edition of the Proceedings of the National Academy of Sciences (PNAS).

"We believe that this method represents a significant breakthrough in gene cloning," wrote the authors of the PNAS study.

"The greatest impact of this technology is likely to be on plants with large and complex genomes, including most crop species," added Sharon R. Long, the William C. Steere, Jr.Pfizer Inc. Professor in Biological Sciences at Stanford and co-author of the study. Long, who also serves as dean of Stanford's School of Humanities and Sciences, is an authority on bacterial and plant molecular biology. She and her colleagues used the new cloning technique to isolate and identify a gene in the DNA of Medicago truncatula, or barrel medic a member of the legume family that is closely related to alfalfa, beans and peas.

"Over the course of six months, we completed what took another group several years to complete, and we identified a pretty cool gene to boot," said Stanford postdoctoral fellow Raka M. Mitra, lead author of the PNAS study. "We think this technology will be applicable to other species and hope that it increases the pace of biological research on the whole."

Reverse genetics

Medicago DNA contai
'"/>

Contact: Mark Shwartz
mshwartz@stanford.edu
650-723-9296
Stanford University
30-Mar-2004


Page: 1 2 3 4 5

Related biology news :

1. Scientists to prototype cyberinfrastructure for research and education access to ocean observatories
2. Scientists sequence genome of kind of organism central to biospheres carbon cycle
3. Scientists find nanowires capable of detecting individual viruses
4. Scientists discover potential new way to control drug-resistant bacteria
5. Scientists explore genome of methane-breathing microbe
6. Scientists decipher genetic code of biothreat pathogen
7. Stuck on you: Scientists lay bare secrets of bacterial attachment proteins
8. Scientists discover proteins involved in spread of HIV-1 infection
9. Scientists fear new Ebola outbreak may explain sudden gorilla disappearance
10. Scientists reinvent DNA as template to produce organic molecules
11. Scientists visualise cellular handmaiden that restores shape to proteins

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/14/2017)... (NYSE: IBM ) is introducing several innovative partner startups ... collaboration between startups and global businesses, taking place in ... nine startups will showcase the solutions they have built with ... France is one of the ... percent increase in the number of startups created between 2012 ...
(Date:5/16/2017)... 16, 2017   Bridge Patient Portal , ... MD EMR Systems , an electronic medical ... GE, have established a partnership to build an ... the GE Centricity™ products, including Centricity Practice Solution ... These new integrations will allow healthcare delivery ...
(Date:4/19/2017)... New York , April 19, 2017 ... competitive, as its vendor landscape is marked by the ... the market is however held by five major players ... Safran. Together these companies accounted for nearly 61% of ... of the leading companies in the global military biometrics ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... ... platform specifically designed for life science researchers to analyze and interpret datasets, ... Franklin, who made a major contribution to the discovery of the double-helix ...
(Date:10/11/2017)... ... ... Personal eye wash is a basic first aid supply for any work environment, but most ... you rinse first if a dangerous substance enters both eyes? It’s one less decision, and ... unique dual eye piece. , “Whether its dirt and debris, or an acid or alkali, ...
(Date:10/11/2017)... , Oct. 11, 2017  VMS BioMarketing, a leading ... a nationwide oncology Clinical Nurse Educator (CNE) network, which will ... need for communication among health care professionals to enhance the ... nurses, office staff, and other health care professionals to help ... breast cancer. ...
(Date:10/11/2017)... ... October 11, 2017 , ... ... implantation and pregnancy rates in frozen and fresh in vitro fertilization (IVF) ... and maternal age to IVF success. , After comparing the results from the ...
Breaking Biology Technology:
Cached News: