HOME >> BIOLOGY >> NEWS
Scientists report first transgenic animal developed via retroviral DNA insertion into male germ-line stem cells

PHILADELPHIA Scientists at the University of Pennsylvania School of Veterinary Medicine have successfully used a retrovirus to modify genes in spermatogonial stem cells in a mouse the first instance, in any species, of a transgenic animal created by inserting a gene into male germ-line stem cells.

The inserted gene subsequently appeared in approximately 4.5 percent of offspring of mice transplanted with the altered stem cells, and was transmitted to at least three succeeding generations.

The work is the cover story in the Nov. 6 issue of the Proceedings of the National Academy of Sciences, and will appear tomorrow on the journals web site. The findings should enable the creation of transgenic individuals in a wide range of species, permitting scientists to develop research models for study of numerous human diseases.

Led by Ralph L. Brinster of Penns Department of Animal Biology, the scientists succeeded in inserting a foreign gene in this case the common reporter gene lacZ, whose product is the enzyme -galactosidase into 2 to 20 percent of mouse spermatogonial stem cells in laboratory experiments, a ten-fold improvement over previous attempts.

"These results indicate that there is no intrinsic barrier to the genetic engineering of spermatogonial stem cells using retroviruses, and that once inserted, the foreign genes will continue to be transmitted and expressed from one generation to the next," said Brinster, the Richard King Mellon Professor of Reproductive Physiology at Penn.

All male mammals harbor many spermatogonial stem cells, key repositories of genetic material whose daughter cells give rise after puberty to sperm. In the human male, approximately 1,000 sperm cells, each carrying a different combination of genetic material, are generated in this manner with each heartbeat. Whereas the female germ cell, the egg, stops dividing before birth, male germ-line stem cells continue to divide throughout
'"/>

Contact: Steve Bradt
bradt@pobox.upenn.edu
215-573-6604
University of Pennsylvania
22-Oct-2001


Page: 1 2 3

Related biology news :

1. Scientists to prototype cyberinfrastructure for research and education access to ocean observatories
2. Scientists sequence genome of kind of organism central to biospheres carbon cycle
3. Scientists find nanowires capable of detecting individual viruses
4. Scientists discover potential new way to control drug-resistant bacteria
5. Scientists explore genome of methane-breathing microbe
6. Scientists decipher genetic code of biothreat pathogen
7. Stuck on you: Scientists lay bare secrets of bacterial attachment proteins
8. Scientists discover proteins involved in spread of HIV-1 infection
9. Scientists fear new Ebola outbreak may explain sudden gorilla disappearance
10. Scientists reinvent DNA as template to produce organic molecules
11. Scientists visualise cellular handmaiden that restores shape to proteins

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/4/2019)... FORT WORTH, Texas (PRWEB) , ... January 02, ... ... 2019 catalog. This release is particularly noteworthy, as the life science tools and ... company’s history, it is offering a catalog. The catalog features not just part ...
(Date:12/25/2018)... ... December 24, 2018 , ... Researchers at ... discovered a new use for a long-standing computational concept known as “blacklisting,” which ... files and messages. Using blacklisting as a filter to single out genetic variations ...
(Date:12/19/2018)... (PRWEB) , ... December 19, 2018 , ... ... of a new study in Arthroscopy evaluating the use of novel ... cuff tears (RCTs). These tears represent a painful, disabling condition for which there ...
Breaking Biology News(10 mins):
(Date:1/4/2019)... ... January 02, 2019 , ... ... precancer and cancer cells in blood, has been named as a partner in ... biotech company developing cell therapies for cancer treatment, will use CellMax Life’s circulating ...
(Date:12/19/2018)... ... December 19, 2018 , ... ... first rate Stem Cell Training Course in Las Vegas. Training includes facial rejuvenation, ... procedures explode in popularity nationwide for aesthetics, having the training from expert, Board ...
(Date:12/18/2018)... ... December 18, 2018 , ... ... optimization firm for the life sciences industry, unveiled a completely redesigned website at ... a more user-friendly browsing experience for their valued clients and business partners. ...
(Date:12/13/2018)... Mass. (PRWEB) , ... December 11, 2018 , ... ... biosensor that doctors and nurses can use to quickly detect Clostridium difficile (C. ... be the heart of a handheld device that can be used onsite in ...
Breaking Biology Technology:
Cached News: