Cold temperature is one of the major factors affecting crop yield in temperate climates, with the farming industry loosing billions of dollars each year to freezing temperatures. Much research has focused on ways to improve crops tolerance to cold and/or freezing temperatures, with the aim to both increase productivity and broaden geographical range.
In 1988, scientists identified the Arabidopsis CBF family of transcription factors. CBF proteins regulate the expression of cold-responsive genes in Arabidopsis, which enable the plant to acclimate to, and survive in, cold temperatures.
As reported in their current G&D paper, Dr. Zhu and colleagues have now discovered a key transcriptional regulator of CBF genes a marked advance in the research effort to understand and ultimately improve cold tolerance in plants.
To identify genes act upon CBF genes and affect cold tolerance in plants, Dr. Zhu and colleagues carried out a genetic screen with Arabidopsis plants that were genetically engineered to glow in the cold. The researchers inserted a luciferase/CBF3 transgene (a recombinant DNA molecule containing the firefly luciferase gene under the control of the CBF3 gene regulatory region) into the Arabidopsis genome, in order to generate plants that bioluminesce under cold stress. These cold-responsive bioluminescent plants were mutagenized, and plants that no longer glowed in cold temperatures were selected.
One particularly striking mutant exhibited ten times less luminescence after 12 hours at 0C than the wild-type bioluminescent plants. Dr. Zhu and colleagues cloned the gene that had been mutated in this
Contact: Heather Cosel
Cold Spring Harbor Laboratory