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Standard improves tests of male DNA

Mother Goose tells us that boys are made of "snips and snails and puppy dog tails." She was clearly misinformed about the snails and tails, but she was on to something with the snips. What you really need to build a boy is a "Y" chromosome, and it turns out that SNPs (single nucleotide polymorphisms), known by the biotech cognoscente as simply "snips," can be helpful in sorting out who fathered the boy. If DNA can be thought of as an instruction book for building a specific person, then SNPs are single letters at an exact location in that book that tend to vary among individuals.

A new Standard Reference Material (SRM) issued by the National Institute of Standards and Technology (NIST) uses both SNPs and STRs (sections where three to five DNA "letters" form repeating patterns) to help improve the reliability of laboratory analyses of male DNA. The result of several years of research, the standard consists of six vials of very carefully analyzed DNA. Five are male samples, and one is female. Laboratories that perform forensics or paternity DNA analyses can use the SRM to double check the accuracy of their equipment and test procedures for analyzing the Y chromosome. It also may be helpful for population studies that study whether the human race evolved from one or many "Adams."

Each vial comes with certified DNA sequences for 22 different STR locations and 42 different SNPs. NIST research chemist Margaret Kline provided a detailed description of the SRM and the methods used in preparing it at the 14th International Symposium on Human Identification in Phoenix, Ariz., on Oct. 1, 2003.


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Contact: Gail Porter
gail.porter@nist.gov
301-975-3392
National Institute of Standards and Technology (NIST)
10-Oct-2003


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