HOME >> BIOLOGY >> NEWS
Steroid-coated DNA represents new approach to gene delivery

PHILADELPHIA Coating DNA with a topical steroid might make for more effective therapeutic gene delivery, according to bioengineers at the University of Pennsylvania. The researchers have shown that a common anti-inflammatory steroid, wrapped around a strand of DNA, can prevent the immune responses commonly associated with gene-transfer techniques.

Studies of the technique, performed in animal models, are presented in the Feb. 15 issue of the journal Gene Therapy, available online now.

"The steroid coating not only allows the gene to be taken up into a cell more easily, but the steroid itself also prevents the sort of inflammatory immune response seen in gene transfer therapy," said Scott Diamond, senior author and professor of bioengineering at Penn and associate director of Penn's Institute for Medicine and Engineering. "The concept paves the way to coupling therapeutic gene delivery with a pharamacological agent, an approach that mitigates some of the drawbacks to the gene-delivery techniques in use now."

Currently there are two basic approaches to delivering therapeutic genes: nonviral and viral. Injecting a subject with pure DNA is possible, but a DNA molecule, by itself, has inherent trouble in entering cells. Viral carriers can serve as delivery vehicles for DNA, literally infecting cells with new genes. Both methods, however, are associated with the creation of inflammatory immune responses that reduces the action of the therapeutic gene.

DNA is a large and negatively charged molecule, which is the source of the stumbling point in getting cells to take up DNA. To counter the negative charge of DNA, Diamond and his colleagues took a common steroid, dexamethasone, and made it "sticky" by adding a nitrogen-rich, postively charged tail. This tail provides the glue that attaches the steroid to the naked DNA.

"The steroid is a fatty lipid so, in essense, we have greased up DNA for cellular uptake," Diamond said,
'"/>

Contact: Greg Lester
glester@pobox.upenn.edu
215-573-6604
University of Pennsylvania
11-Feb-2004


Page: 1 2

Related biology news :

1. Belgian researchers explore revolutionary approach to angiogenesis
2. Novel IBD therapeutic approaches reported from Washington Univ., Barcelona, LSU at APS meeting
3. Researchers recommend new approach to combat drug-resistant staph infections
4. U. Iowa muscular dystrophy discovery may lead to new treatment approaches
5. New research suggests therapeutic approach for autoimmune diseases
6. New approach offers potential drug-discovery shortcut
7. New approach limits damage after heart attack and improves survival, say Scripps Research scientists
8. Two-drug approach might shorten painful labor, reduce Caesarean sections
9. Death-defying approach devised by Penn scientists to prevent cell apoptosis
10. Smallpox in 50-year-old tissues detected by integrated diagnostics approach
11. One-size-fits-all approach to nutrition recommendations may soon be outdated

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/10/2016)... March 10, 2016   Unisys Corporation (NYSE: ... Protection (CBP) is testing its biometric identity solution at ... to help identify certain non-U.S. citizens leaving the ... test, designed to help determine the efficiency and accuracy of ... February and will run until May 2016. --> ...
(Date:3/9/2016)... BEACH GARDENS, Fla. , March 9, 2016 ... identity management authentication and enrollment solutions, today announced ... DigitalPersona ® Altus multi-factor authentication ... IT and InfoSec managers to step-up security where ... Washington, DC . ...
(Date:3/3/2016)... , March 3, 2016  2016FLEX, organized by ... week highlighting advancements in flexible, hybrid and printed ... setting attendance - have gathered for short courses, ... field of electronics. The Flex Conference celebrates its ... for companies, R&D organizations, and universities contributing to ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... May 26, 2016 Q BioMed Inc. ... will be a featured presenter at the 5th Annual Marcum ... New York City at the Grand Hyatt Hotel. ... Q BioMed Inc. CEO, is scheduled to begin at 11a.m ... company,s business strategy, recent developments and outline milestones for the ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lajollacooks4u has become a rising hotspot for ... one of its top attractions. Fortune 500 companies, such as Illumina, Hewlett-Packard, Qualcomm ... and intimate team-building experience. , Each event kicks off with an olive oil and ...
(Date:5/25/2016)... ... ... at the University of Athens say they have evidence that the variety of different ... lead to one good one. Surviving Mesothelioma has just posted an article on the ... 98 mesothelioma patients who got a second kind of drug therapy after ...
(Date:5/24/2016)... 24, 2016   MedyMatch Technology Ltd ., the data ... real-time decision support tools in the emergency room, announced today ... Israeli Advanced Technology Industries (IATI) BioMed Conference. ... 15th National Life Sciences and Technology Week, and is ... Hotel in Tel Aviv, Israel . ...
Breaking Biology Technology:
Cached News: