HOME >> BIOLOGY >> NEWS
Steroid-coated DNA represents new approach to gene delivery

PHILADELPHIA Coating DNA with a topical steroid might make for more effective therapeutic gene delivery, according to bioengineers at the University of Pennsylvania. The researchers have shown that a common anti-inflammatory steroid, wrapped around a strand of DNA, can prevent the immune responses commonly associated with gene-transfer techniques.

Studies of the technique, performed in animal models, are presented in the Feb. 15 issue of the journal Gene Therapy, available online now.

"The steroid coating not only allows the gene to be taken up into a cell more easily, but the steroid itself also prevents the sort of inflammatory immune response seen in gene transfer therapy," said Scott Diamond, senior author and professor of bioengineering at Penn and associate director of Penn's Institute for Medicine and Engineering. "The concept paves the way to coupling therapeutic gene delivery with a pharamacological agent, an approach that mitigates some of the drawbacks to the gene-delivery techniques in use now."

Currently there are two basic approaches to delivering therapeutic genes: nonviral and viral. Injecting a subject with pure DNA is possible, but a DNA molecule, by itself, has inherent trouble in entering cells. Viral carriers can serve as delivery vehicles for DNA, literally infecting cells with new genes. Both methods, however, are associated with the creation of inflammatory immune responses that reduces the action of the therapeutic gene.

DNA is a large and negatively charged molecule, which is the source of the stumbling point in getting cells to take up DNA. To counter the negative charge of DNA, Diamond and his colleagues took a common steroid, dexamethasone, and made it "sticky" by adding a nitrogen-rich, postively charged tail. This tail provides the glue that attaches the steroid to the naked DNA.

"The steroid is a fatty lipid so, in essense, we have greased up DNA for cellular uptake," Diamond said,
'"/>

Contact: Greg Lester
glester@pobox.upenn.edu
215-573-6604
University of Pennsylvania
11-Feb-2004


Page: 1 2

Related biology news :

1. Belgian researchers explore revolutionary approach to angiogenesis
2. Novel IBD therapeutic approaches reported from Washington Univ., Barcelona, LSU at APS meeting
3. Researchers recommend new approach to combat drug-resistant staph infections
4. U. Iowa muscular dystrophy discovery may lead to new treatment approaches
5. New research suggests therapeutic approach for autoimmune diseases
6. New approach offers potential drug-discovery shortcut
7. New approach limits damage after heart attack and improves survival, say Scripps Research scientists
8. Two-drug approach might shorten painful labor, reduce Caesarean sections
9. Death-defying approach devised by Penn scientists to prevent cell apoptosis
10. Smallpox in 50-year-old tissues detected by integrated diagnostics approach
11. One-size-fits-all approach to nutrition recommendations may soon be outdated

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Steroid coated DNA represents new approach gene delivery

(Date:5/18/2015)... , May 18, 2015 Fingerprint Cards ... FPC1020, FPC1025, FPC1145, FPC1155 and FPC1035 from one if its ... until and including Q3 2015 and the sensors will be ... Communicated order values for 2015 hereby amount to 740 MSEK ... 2015 of 140 MSEK and a number of smaller orders ...
(Date:5/11/2015)... DAYTON, Ohio , May 11, 2015  Through a ... Ohio had a strong showing at AUVSI,s ... . These representatives of Ohio,s UAS ... domestic and abroad from all points along the UAS ecosystem. ... Dayton Development Coalition,s (DDC) Vice President for Aerospace Rich ...
(Date:5/7/2015)... Sweden , May 7, 2015 ... touch fingerprint sensors, FPC1022 and FPC1035, FPC,s smallest ... and FPC1035 are mainly considered for integration on ... size gives smartphone OEMs increased possibilities to integrate ... The decreased size also improves possibilities for module ...
Breaking Biology News(10 mins):Fingerprint Cards Receives Touch Fingerprint Sensor Order of SEK 230 Million and Raises Revenue Guidance for 2015 2Ohio Flies High at Unmanned '15, Sets Stage for Ohio UAS Conference 2FPC Introduces its Smallest Touch Fingerprint Sensors to Date 2
(Date:5/29/2015)... May 29, 2015 Seoul Semiconductor ... in LED technology, on May 28th announced that ... share by strengthening its high efficient and high ... , Since 2011 Seoul Semiconductor has developed efficient ... market and has supplied LEDs for headlamps to ...
(Date:5/28/2015)... , May 28, 2015 /PRNewswire/ ... http://www.researchandmarkets.com/research/8h5hs2/animal ) has announced the addition ... Biotechnology - Technologies, Markets and Companies" ... , ,This report describes and evaluates ... veterinary medicine and pharmaceuticals as well ...
(Date:5/28/2015)... 2015 The Academy of Model Aeronautics Foundation ... on August 15, 2015. National Model Aviation Day was created ... introduce model flying to the general public. , The AMA ... participate in the celebration by hosting events in their own ... Model Aviation Day events. View our online event map ...
(Date:5/28/2015)... , May 28, 2015  PDS Biotechnology Corp. ... immunotherapy treatment targeting HPV-16, has generated strong T-cell ... Currently, pre-cervical cancer is treated by surgical removal ... and effective non-surgical alternative. Results show that it ... to recognize, target, and kill precancerous and cancerous ...
Breaking Biology Technology:Seoul Semiconductor Increases the Global Automotive LED Market Share with its High Efficient and Reliable LEDs for Automotive Lightings 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 2Global Animal Biotechnology Report 2015 - Technologies, Markets and Companies Analysis to 2024 3Model flying pilots celebrate the Academy of Model Aeronautics Foundation’s Third Annual National Model Aviation Day 2PDS Biotechnology reports positive Phase I clinical trial results in proprietary HPV-Cancer Immunotherapy 2
Cached News: