HOME >> BIOLOGY >> NEWS
Stretching DNA on a tiny scale, researchers probe the basis for its compaction

Using magnets and video microscopy to measure the length of individual DNA molecules under experimental conditions, researchers have demonstrated that Condensin, a complex of proteins widely conserved in evolution, physically compacts DNA in a manner dependent on energy from ATP. The finding is significant because the Condensin complex, which is essential for life, has been known to play a key role in the dramatic condensation of genomic DNA that precedes mitosis and cell division. The new work puts into sharper focus the mechanism by which Condensin accomplishes this compaction, which is essential for the precise segregation of the genetic material to later generations of cells.

Scientists Terence Strick, Tatsuhiko Kawaguchi and Tatsuya Hirano of Cold Spring Harbor Laboratory employed a nanomanipulation technique by which small individual molecules of DNA, tethered on one end to a glass slide and attached on the other end to a magnetic bead, could be gently stretched and twisted using small magnets. The technique allowed the researchers to exert controlled, variable force on the extended DNA, directly measuring changes in its compaction following interactions with Condensin complexes isolated from frog eggs. Because the helical DNA could be twisted, the scientists were also able to investigate how DNA topology in this case, topological states called positive and negative supercoiling might affect its ability to be compacted by Condensin. Such measurements are central to illuminating the molecular mechanism used by Condensin in the cell.

The researchers found that Condensin compacts DNA against a weak stretching force, but that increasing the force on the DNA reversed compaction, effectively breaking apart the molecular interactions formed by Condensin. Carefully measuring changes in distance between the two ends of the DNA molecule revealed evidence that both compaction and decompaction often occurred in jumps of certain lengths. Comparing the ran
'"/>

Contact: Heidi Hardman
hhardman@cell.com
617-397-2879
Cell Press
22-Apr-2004


Page: 1 2

Related biology news :

1. Belgian researchers explore revolutionary approach to angiogenesis
2. Award winning researchers reveal potential new role for Glivec
3. $7.5 Million grant to Yale researchers for role of viruses in cancer
4. New anti-inflammatory strategy for cancer therapy identified by UCSD researchers
5. Joslin researchers clarify mechanisms for beta-cell formation
6. Virginia Tech researchers to release findings on Smith River Project
7. Molecular motor myosin VI moves hand over hand, researchers say
8. ASU researchers demonstrate new technique that improves the power of atomic force micrscopy
9. Emory researchers map structure of anti-cancer molecule
10. Leukemia stem cells identified by Stanford researchers
11. EURYI Award given for the first time to 25 young European researchers

Post Your Comments:
(Date:6/15/2015)... DALLAS , June 16, 2015 ... Language Processing Market by Type (Rule-Based, Statistical, and Hybrid), ... Worldwide Forecast to 2020" published by MarketsandMarkets, The Natural ... Billion at a CAGR of 18.4% for the forecast ... Tables and   46 F igures spread   ...
(Date:6/12/2015)... , June 12, 2015  Securus Technologies, a ... solutions for public safety, investigation, corrections and monitoring, ... patent to electronically capture, analyze and identify participants ... recognition methods. This system can: ... an enrollment and subsequent sign-on process is actually ...
(Date:6/9/2015)... 9, 2015   MedNet Solutions , an innovative ... of clinical research, will be providing demonstrations of its ... the 2015 Drug Information Association (DIA) Annual Meeting in ... These presentations will include previews of many exciting new ... upcoming iMedNet 2015 Feature 1 Release. ...
Breaking Biology News(10 mins):Natural Language Processing Market Worth $13.4 Billion by 2020 2Natural Language Processing Market Worth $13.4 Billion by 2020 3Natural Language Processing Market Worth $13.4 Billion by 2020 4Natural Language Processing Market Worth $13.4 Billion by 2020 5Securus Announces Patent Issued for Authorized Presence Verification During Video Visitation 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 2MedNet To Provide Sneak Peeks Of Its iMedNet "2015 Feature 1 Release" At The DIA Annual Meeting 3
(Date:7/1/2015)... CA (PRWEB) , ... July 01, 2015 , ... It ... brain injury (TBI) using single photon emission computed tomography (SPECT) according to ... The study, which examined over 20,000 brain scans and is believed to be the ...
(Date:7/1/2015)... July 1, 2015   Decision Resources Group ... advantages of Otezla are its oral administration, alternative ... reported by surveyed rheumatologists. Otezla was the first ... treatment of active psoriatic arthritis in ... of care includes conventional oral treatments including disease-modifying ...
(Date:7/1/2015)... BANGALORE, India , July 1, 2015 ... that uses next generation sequencing technology to empower cancer ... Storrer as chief executive officer. In his expanded ... responsible for all strategy, business expansion and worldwide operations ... served as Strand,s chairman and CEO since its founding ...
(Date:7/1/2015)...  Axovant Sciences Ltd. (NYSE:  AXON), a leading ... dementia, today announced that the company will host ... p.m. EDT following its presentation of RVT-101 clinical ... (AAIC). The event will include ... Lawrence Friedhoff , Chief Development Officer, will present ...
Breaking Biology Technology:Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 2Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 3Brain Scans Can Tell Post-Traumatic Stress Disorder and Traumatic Brain Injury Apart According to New Research by Amen Clinics 4Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 2Strand Life Sciences Names Scott A. Storrer CEO as Company Continues Global Expansion with Focus on Precision Medicine Product 3Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 2Axovant Sciences To Host Investor Briefing And Live Webcast During AAIC 2015 Meeting 3
Cached News: