HOME >> BIOLOGY >> NEWS
Structure shows repair protein cradling broken DNA

But actually, the non-homologous end joining process is quite accurate, and we wanted to find out why Ku appears to be so necessary for that accuracy.

Goldberg and his colleagues believed that seeing how Ku binds to DNA might provide answers to some of the questions about the Ku-DNA interaction. The researchers used a technique called x-ray crystallography to visualize the interplay between the Ku heterodimer and DNA. In x-ray crystallography, protein crystals are bombarded with intense x-ray beams. As the x-rays bounce off atoms in the crystal, they leave a diffraction pattern, which can then be analyzed to determine the three-dimensional structure of the protein.

Before they could get a full picture of the Ku-DNA complex, Goldberg and his colleagues decided to study the Ku heterodimer by itself. Their attempts to prepare crystals of the Ku protein yielded only a few usable crystals out of the hundreds they prepared. Fortunately, the scientists were able to use a technique pioneered by HHMI investigator Wayne Hendrickson to solve the complete structure of the Ku heterodimer from a single crystal. The technique, called multiple wavelength anomalous diffraction, was applied during crystallographic analyses performed at the National Synchrotron Light Source at Brookhaven National Laboratory.

After the Ku structure was determined, the scientists moved on to solving the structure of the Ku-DNA complex. In their studies, Goldberg and his colleagues had to mimic DNA breakage, ensuring that their test DNA fragment had only one accessible end -- in order to avoid Ku attaching at more than one site on the DNA. They accomplished this by blocking the other end of the DNA with a bulky DNA motif.

After solving the structure of Ku bound to DNA, Goldberg and his colleagues could see how the Ku heterodimer manages to find a broken DNA end regardless of its sequence. The problem is that Ku is not like a transcription factor that binds to a specific DNA sequence,
'"/>

Contact: Jim Keeley
keeleyj@hhmi.org
301-215-8858
Howard Hughes Medical Institute
8-Aug-2001


Page: 1 2 3

Related biology news :

1. Structure solved at Scripps shows how one human protein reduces potency of chemotherapy
2. Structure solved by Scripps scientists shows one way that body controls gene expression
3. Structure of a Nobel-prize winning molecule: Aquaporin
4. Structure of HIV-neutralizing antibody solved
5. Structure of key breast cancer receptor determined
6. Structure of cog at the hub of metabolism reveals anti-ageing function
7. Structure of pain-modulating enzyme described by scientists at the Scripps Research Institute
8. Structure reveals details of cells cargo-carriers
9. Structure of key receptor unlocked; Related proteins will fall like dominoes
10. New Science Press launches Proteins: From Sequence to Structure
11. Third piece completes deadly puzzle: Structure of anthrax toxin offers clues to treatment

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/22/2016)... India , March 22, 2016 /PRNewswire/ ... market research report "Electronic Sensors Market for Consumer ... Proximity, & Others), Application (Communication & IT, ... Geography - Global Forecast to 2022", published ... industry is expected to reach USD 26.76 ...
(Date:3/17/2016)... , March 17, 2016 ABI Research, ... forecasts the global biometrics market will reach more ... 118% increase from 2015. Consumer electronics, particularly smartphones, ... fingerprint sensors anticipated to reach two billion shipments ... Dimitrios Pavlakis , Research Analyst at ABI ...
(Date:3/14/2016)... March 14, 2016 http://www.apimages.com ... --> - Renvoi : image disponible via ... --> --> DERMALOG, le ... de nouveaux lecteurs d,empreintes digitales pour l,enregistrement des ... sera utilisé pour produire des cartes d,identité aux ...
Breaking Biology News(10 mins):
(Date:4/27/2016)... NY (PRWEB) , ... April 27, 2016 , ... ... it. Touch screen mobile devices with fingerprint recognition for secure access, voice ... are only a few ways consumers are interacting with biometrics technology today. ...
(Date:4/27/2016)... ... April 27, 2016 , ... ... its Scientific Advisory Board. Dr. Lamka will assist PathSensors in expanding the use ... , PathSensors deploys the CANARY® test platform for the detection of harmful pathogens, ...
(Date:4/27/2016)... , April 27, 2016 ... research report with specific focus on US, EU, ... Japan , to the healthcare business intelligence ... library. Complete report on the Flow ... companies and supported with 282 tables and figures ...
(Date:4/26/2016)... ... April 26, 2016 , ... Mr. Palmer created the RPO business for Ceridian ... dollar, multi-year managed services contract in the U.S. intelligence community with The SI (a ... said John Younger, founder of Accolo. “We are growing and his experience guiding ...
Breaking Biology Technology:
Cached News: