HOME >> BIOLOGY >> NEWS
Structure shows repair protein cradling broken DNA

But actually, the non-homologous end joining process is quite accurate, and we wanted to find out why Ku appears to be so necessary for that accuracy.

Goldberg and his colleagues believed that seeing how Ku binds to DNA might provide answers to some of the questions about the Ku-DNA interaction. The researchers used a technique called x-ray crystallography to visualize the interplay between the Ku heterodimer and DNA. In x-ray crystallography, protein crystals are bombarded with intense x-ray beams. As the x-rays bounce off atoms in the crystal, they leave a diffraction pattern, which can then be analyzed to determine the three-dimensional structure of the protein.

Before they could get a full picture of the Ku-DNA complex, Goldberg and his colleagues decided to study the Ku heterodimer by itself. Their attempts to prepare crystals of the Ku protein yielded only a few usable crystals out of the hundreds they prepared. Fortunately, the scientists were able to use a technique pioneered by HHMI investigator Wayne Hendrickson to solve the complete structure of the Ku heterodimer from a single crystal. The technique, called multiple wavelength anomalous diffraction, was applied during crystallographic analyses performed at the National Synchrotron Light Source at Brookhaven National Laboratory.

After the Ku structure was determined, the scientists moved on to solving the structure of the Ku-DNA complex. In their studies, Goldberg and his colleagues had to mimic DNA breakage, ensuring that their test DNA fragment had only one accessible end -- in order to avoid Ku attaching at more than one site on the DNA. They accomplished this by blocking the other end of the DNA with a bulky DNA motif.

After solving the structure of Ku bound to DNA, Goldberg and his colleagues could see how the Ku heterodimer manages to find a broken DNA end regardless of its sequence. The problem is that Ku is not like a transcription factor that binds to a specific DNA sequence,
'"/>

Contact: Jim Keeley
keeleyj@hhmi.org
301-215-8858
Howard Hughes Medical Institute
8-Aug-2001


Page: 1 2 3

Related biology news :

1. Structure solved at Scripps shows how one human protein reduces potency of chemotherapy
2. Structure solved by Scripps scientists shows one way that body controls gene expression
3. Structure of a Nobel-prize winning molecule: Aquaporin
4. Structure of HIV-neutralizing antibody solved
5. Structure of key breast cancer receptor determined
6. Structure of cog at the hub of metabolism reveals anti-ageing function
7. Structure of pain-modulating enzyme described by scientists at the Scripps Research Institute
8. Structure reveals details of cells cargo-carriers
9. Structure of key receptor unlocked; Related proteins will fall like dominoes
10. New Science Press launches Proteins: From Sequence to Structure
11. Third piece completes deadly puzzle: Structure of anthrax toxin offers clues to treatment

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/21/2017)... N.Y. and PORTLAND, Ore. ... ) and the Avamere Family of Companies (Avamere Health ... today announced a six-month research study that will apply ... improve eldercare at senior living and health centers. By ... Avamere hopes to gain insights into physical and environmental ...
(Date:2/21/2017)... -- Der weltweite Biobanking-Sektor wird bis zum ... mit mehr als 50 Vertretern aus verschiedenen Branchen wurde aber ... diese Prognose zu realisieren. ... Zu den Schwierigkeiten für ... für die Biobank, die Implementierung Zeit sparender Technologien, ein ...
(Date:2/16/2017)... FRANCISCO , Feb. 16, 2017  Genos, ... today announced that it has received Laboratory Accreditation ... CAP Accreditation is presented to laboratories that meet ... who demonstrate scientifically rigorous processes. "Genos ... excellence in laboratory practices. We,re honored to be ...
Breaking Biology News(10 mins):
(Date:2/21/2017)... 2017 Synthetic Biologics, Inc. (NYSE MKT: SYN), ... to protect and restore the health of patients, intends to report ... 2016 on Thursday, March 2, 2017, and to host a conference ... for the call is as follows: U.S. (toll free): ... ...
(Date:2/21/2017)... , Feb. 21, 2017 /PRNewswire/ - SQI Diagnostics Inc. ("SQI" or ... operational results for the three months ended December 31, 2016. ... -based life sciences and diagnostics company that develops and commercializes ... ... to build on the commercial milestones achieved in fiscal 2016," ...
(Date:2/21/2017)... ... February 21, 2017 , ... ... to build connected digital health applications, announced a partnership with Redox, a leader ... seamlessly connect to many clinical systems while keeping data secure in the cloud. ...
(Date:2/21/2017)... PARK, CA (PRWEB) , ... February 21, 2017 ... ... of the VTX-1 Liquid Biopsy System , a fully automated benchtop system ... microfluidic technology. The VTX-1 is being launched at the Molecular Medicine Tri Conference ...
Breaking Biology Technology:
Cached News: