HOME >> BIOLOGY >> NEWS
The RANKL cytokine at 2.6

The tumor necrosis factor (TNF) superfamily represents a large, loosely related, and versatile group of homotrimeric cytokines. There has been considerable interest in the structure of these proteins, and X-ray analyses are available for several of them, both in isolated form and as co-crystals with their corresponding receptors. Still, the substantial divergence in primary sequence within this family has made it difficult to predict the structure of other members with confidence. Here, Lamn et al. report a high-resolution crystal structure of the RANK ligand (RANKL), a TNF relative that acts through its receptor (RANK) to promote the differentiation and bone-resorptive activity of osteoclasts and to block apoptosis by several other cell types. As with other members of this superfamily, RANKL monomers trimerize only with each other, even when they are coexpressed with other related cytokines. Likewise, the mature trimeric protein binds specifically to RANK and not to other members of the TNF receptor family. The present crystal structure goes far toward explaining these biochemical observations. While the protein folds to form a characteristic structure that has been seen in related cytokines, there are also several solvent-exposed loops with sequences not found in other family members. Based on the well defined quaternary structure of receptors of this family, Lamm et al. predicted that one of these loops binds directly to RANK. To confirm this hypothesis, the authors mutated RANKL so that it either entirely lacks the loop in question or bears in its place a sequence from TNF. As expected, the resulting proteins form trimers normally but are incapable of interacting with RANK. This loop sequence would therefore be expected to antagonize RANK signaling and might prove useful in controlling ostoeporosis by blocking osteoclast-mediated bone resorption.


'"/>

Contact: John Ashkenas
scied@the-jci.org
416-946-7593
Journal of Clinical Investigation
26-Sep-2001


Page: 1

Related biology news :

1. Lowly cytokine may play role in controlling neurotransmitters
2. Role of cytokine inhibition in chronic heart failure

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2020)... ... ... In its June 22 online post, business and technology news platform Superb Crew ... and director of stem cell biotechnology company Asymmetrex . Superb Crew decided to ... 5 it would begin offering free tissue stem cell counting at its ...
(Date:6/19/2020)... ... June 18, 2020 , ... ... “Automate Your Lab with Software for Data Decisions,” for pharmaceutical and biotech ... to integrate and automate cutting-edge scientific instruments and laboratory equipment from dozens ...
(Date:6/13/2020)... ... 2020 , ... Bode Technology (Bode), a leading ... team. Bode’s Forensic Genealogy Service (FGS) continues to deliver investigative leads ... The team has added experienced genealogists, each having over a decade of field ...
Breaking Biology News(10 mins):
(Date:6/28/2020)... Calif. (PRWEB) , ... June 25, 2020 , ... The ... the launch of Safe Space™ for businesses of all types to ensure cleanliness, distancing ... LiDAR. , A Safe Space™ enabled business will provide a health performance ...
(Date:6/28/2020)... Conn. (PRWEB) , ... June 25, 2020 , ... ... formed to advance photodynamic therapy for treating cancer, today announced the company has ... York (“Roswell Park”). The agreement provides Lumeda globally exclusive rights to Roswell ...
(Date:6/23/2020)... ... , ... Kerafast Inc. , developers of an online ... of the Delta-G-VSV Pseudotyping System for coronavirus research applications. The system, developed by ... entry and COVID-19 vaccine effectiveness at just biosafety level 2 (BSL-2) containment. , ...
(Date:6/23/2020)... ... June 22, 2020 , ... Regen Suppliers, the one stop ... buy three, get the fourth unit free with no limit on the amniotic ... as exosomes, have been a game changer for regenerative therapies. The products are ...
Breaking Biology Technology:
Cached News: