The first engineering of cell surfaces in living animals

ave harmful effects -- indeed they are useful in some drugs: the A in AZT, azidothymidine, is for azide -- but they rapidly react with each other under physiological conditions. Suitably modified by Bertozzi's methods, the ligation product formed from these compounds is stable under physiological conditions.

The trick is to get the azide half of the reaction to show up on cell surfaces. In cell cultures, this is done by diffusing into the cells a precursor, containing the azide group, of a commonly expressed form of oligosaccharide, or sugar, that includes sialic acid. The cells manufacture these azide-bearing sugars and display them on their surfaces.

With mice, Bertozzi's group injected the azide-containing precursor directly into the abdomen, where cells in the spleen and some other organs took it up. Later examination of spleen cells, which are rich in sialic-acid sugars, showed an abundance of cell-surface azides.

Because rodent blood has high levels of esterase, enzymes that might interfere with the conversion of the modified precursor, the first experiments were done with esterase-deficient knockout mice. Later the investigators found that wild-type mice displayed just as many cell-surface azides, so esterase was apparently no hindrance to the uptake of the azide-containing precursor.

The other half of the Staudinger ligation, the phosphine, may be attached to a great variety of markers. To confirm the success of the Staudinger ligation in mice, Bertozzi and her collaborators used a peptide that could be identified by a fluorescing antibody.

"The future holds a range of opportunities for using the Staudinger ligation for noninvasive imaging," says Bertozzi. "Phosphine markers might include radiochemicals for detection by positron emission tomography, or magnetically active molecules for detection by magnetic resonance imaging, or nanocrystals with unique optical properties, or many other methods. These markers cou

Contact: Paul Preuss
DOE/Lawrence Berkeley National Laboratory

Page: 1 2 3

Related biology news :

1. New study indicates arsenic could be suitable as first-line treatment in type of leukaemia
2. Newly identified master gene key in babys first breath
3. The book opens on the first tree genome
4. DuPont developing new protective suits for military, first responders
5. Joslin Diabetes Center adds first affiliate in California at Irvine Medical Center
6. Maine-based biophysics institute gets funding for first US-based 4Pi nanoscale microscope
7. Veterinarians discover first known case of canine distemper in a wild tiger
8. Study reveals first genetic step necessary for prostate cancer growth
9. Infant rats eagerly accept high concentrations of alcohol upon first exposure
10. Newcastle scientists get UKs first stem cell license
11. EURYI Award given for the first time to 25 young European researchers

Post Your Comments:

(Date:6/18/2019)... LONG BEACH, Calif. (PRWEB) , ... June 18, ... ... provider of multi-GPU deep learning cloud solutions, today introduced Data Science Workstations-as-a-Service as ... data science workstation hardware from BOXX Technologies, Cirrascale Data Science Workstations feature NVIDIA® ...
(Date:6/14/2019)... ... , ... Molecular diagnostics segment is the fastest-growing segment within the global IVD ... segments that are expected to drive the growth are infectious diseases, cancer, and transplant ... Kalorama’s The World Molecular Diagnostics Market, 8th Edition . , The discovery of ...
(Date:6/11/2019)... ... June 12, 2019 , ... With a rapidly growing ... leverage the Geneious Biologics platform as a premium hub for bioinformatics and computer ... says: “The Geneious team has done an excellent job accommodating our specific data, ...
Breaking Biology News(10 mins):
(Date:5/31/2019)... ... May 30, 2019 , ... A study released ... delivery system for the sustained release of human placental stem cell (HPSC)-derived conditioned ... able to deliver CM into the injured kidney, where it helped restore function ...
(Date:5/14/2019)... ... May 13, 2019 , ... Leak Detection ... for the Pharmaceutical, Biotechnology, Medical Device and Food Packaging Industries’ is excited to ... aims to improve LDA’s ability to communicate directly with the marketplace; serving as ...
(Date:5/4/2019)... , ... May 03, 2019 , ... ... release of the Diopsys® ffERG/Photopic Negative Response vision test, a new ... By 2050, it is estimated that 6.3 million Americans will have glaucoma, with ...
(Date:5/2/2019)... ... May 02, 2019 , ... Boston Robotic ... Boston area. Gone are the days of painful hair transplant procedures. Boston Robotic ... System is the only robotic intelligent solution to offer a minimally invasive procedure. ...
Breaking Biology Technology:
Cached News: