Translational repression in germline d...( from binding with a different partneri...)

from binding with a different partnering molecule, eIF4G, and thereby inhibits the initiation of translation. Findings that a mutant form of Cup lacking the sequence with which it binds eIF4E failed to repress oskar translation in vivo, that Cup interacts with Bruno in a yeast two-hybrid assay, and that the Cup-eIF4E complex associates with Bruno in an immunoprecipitation assay, suggesting that these three proteins form a complex that achieves translational repression by interactions with both the 3' and 5' ends of the oskar RNA. A similar model of protein interactions is observed in the translational repression of the cyclin-B1 RNA in the Xenopus African clawed frog, indicating that the paradigm of translational repression through the 5'/3' interactions is conserved across species.

Nakamura next intends to look into the means by which the repressor effects of the eIF4E-Cup-Bruno complex are alleviated at the appropriate developmental stage, after the oskar ribonucleoprotein complex has reached and anchored to its appropriate destination at the pole of the oocyte.

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Contact: Doug Sipp
sipp@cdb.riken.jp
81-78-306-3043
RIKEN Center for Developmental Biology
12-Jan-2004

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