UCSF/Gladstone Finding May Explain HIV'S Ability To Infect Cells Lacking The Key Target Of HIV: The CD4 Receptor

e culture experiments. The answer was yes, determined by substituting a recombinant protein containing solely this extra-cellular portion of CD4 (provided by collaborators at SmithKline Beecham) for the CD4-positive cells.

When the CCR5-bearing cell was mixed with the soluble CD4 (sCD4) protein, the researchers found clear evidence of infection. "A functional receptor complex was generated, indicating that the complex could be formed without CD4 being anchored in a particular cell membrane," said Goldsmith.

All that mattered was that the outside portion of the CD4 molecule be available in close proximity to the co-receptor-bearing cell.

"With further studies, we even showed that it required only a small fragment of the outside portion of CD4, known as the D1 domain. Apparently, CD4, or even just a portion of it, when juxtaposed with the co-receptor on a target cell, can reconstitute a full permissive system," he said.

The researchers suspect that the extra-cellular portion of CD4, the "ectodomain," may be physically juxtaposed with CCR5 on these CD4-negative cells, and that virus floating by may see this physical interaction as a single receptor complex.

"It may be that HIV doesn't have any way of realizing that these receptors are actually on different cells," said Goldsmith. It appears to be yet another devious pathway by which HIV further insinuates itself into the host."

An important step the researchers hope to take is to determine what types of cells in humans can cooperate in this way, said Goldsmith. "We did one such research effort using human brain cells in laboratory cultures, and found that the system did work, giving us compelling evidence that this pathway might be applicable during typical infections of the brain."

Co-authors of the study were Roberto Speck, MD; Ursula Esser, PhD; Michael L. Penn, BA; Daniel A. Eckstein, BA, and Stephen Chan, BA, from the UCSF-affiliated Gladstone Institute of Virology and Immunolo

Contact: Jennifer O'Brien
University of California - San Francisco

Page: 1 2 3 4

Related biology news :

1. AIDS Virus May Evolve Differently In Cerebrospinal Fluid Compared With Blood In Some Patients, UCSF/Gladstone Research Finds
2. Breakthrough In Understanding The Biology Of Fat-- UCSF/Gladstone Scientists Discover Gene For Key Enzyme
3. Findings suggest need for new view of p53 cancer proteins interaction with DNA
4. Finding the hole in the defenses of cavity-creating microbes
5. Findings redefine mechanism of action of RNA helicase enzymes
6. Findings of novel nanoproperties in selenium produced by bacteria open new area of exploration
7. Finding may help eczema sufferers tolerate smallpox vaccine
8. Findings could aid efforts to harness nature for making drugs
9. Findings offer clue to how molecule can both stimulate, suppress cell growth
10. Findings offer further understanding about growth and development in young male gymnasts
11. Findings in frog oocytes may help study of chromosome physiology

Post Your Comments:

(Date:7/1/2020)... ... July 01, 2020 , ... Cure Glioblastoma, a registered ... common and aggressive adult brain cancer—announced today the appointments of its first Senior ... with supporting the organization’s initiatives and overall vision. , "Senior Fellows are more ...
(Date:6/23/2020)... ... June 23, 2020 , ... ... today announced that the company has received ISO9001:2015 certification for the development ... research industries. The decision to pursue ISO9001 accreditation demonstrates Kemp’s commitment to ...
(Date:6/23/2020)... ... 23, 2020 , ... In its June 22 online post, business and technology ... Sherley, M.D., Ph.D., founder and director of stem cell biotechnology company Asymmetrex . ... that starting July 5 it would begin offering free tissue stem cell ...
Breaking Biology News(10 mins):
(Date:7/10/2020)... ... 2020 , ... In most research using the zebrafish model, ... in zebrafish embryos, maintaining optical transparency to facilitate microscopic imaging. Over the past ... the zebrafish model to investigate the causes of leukaemia and its relationship with ...
(Date:7/7/2020)... , ... July 06, 2020 , ... R3 International is ... featuring up to 200 million stem cells. Depending on the patient's condition, treatment may ... the US will die having some form of Alzheimers dementia, and the incidence continues ...
(Date:7/1/2020)... ... June 29, 2020 , ... MedShift , a ... 2020, despite many obstacles created as a result of COVID-19. As a provider ... partnered medical practices and medical manufacturers by expanding access to device offerings, technology ...
(Date:7/1/2020)... ... June 30, 2020 , ... ... that the launch of a new clinical diagnostics immuno-oncology service, TissueInsight ... microenvironment (TME). , “Flagship’s TissueInsight is a service that aids pathologists, oncologists, ...
Breaking Biology Technology:
Cached News: