HOME >> BIOLOGY >> NEWS
UI Laboratory Develops Procedure To Study DNA Replication

IOWA CITY, Iowa -- A University of Iowa research team has developed a way to isolate replicating deoxyribonucleic acid (DNA) molecules that scientists then can use to examine the replication process under controlled conditions. This advance will allow investigators to better understand DNA replication and may lead to improved therapies for treating diseases such as cancer.

"One of the difficulties with studying the DNA replication process is that there are multiple protein complexes involved, and it is difficult to experimentally dissect individual reactions that are occurring," said Marc Wold, Ph.D., UI associate professor of biochemistry and the project's principal investigator. "Our procedure will allow us to examine these reactions in more detail."

DNA is the genetic material that encodes all components in human cells. Each cell contains more than two meters of DNA. Every time a cell divides, it is necessary to duplicate all of its DNA.

Using DNA derived from the Simian Virus 40 from monkeys, Wold and members of his laboratory let DNA replication start in an extract from human cells. The researchers attach magnetic beads to the DNA molecules and use a magnet to isolate the molecules and associated replication proteins. The researchers can then manipulate the extracted DNA and add specific replication proteins in a controlled manner to understand the mechanics of DNA replication.

"This will mean we can start asking specific questions about the reactions during replication and learn more about the specific roles of replication proteins," Wold said.

The advantage of this system is that it allows separation of the DNA replication process from the synthetic reactions. This separation has been difficult to achieve in previous replication systems.

Using his technique, Wold hopes that his lab and others can begin to understand what causes some cells to mutate. DNA replication is a complex process that is very accurate and highly regulate
'"/>

Contact: Jennifer Cronin
jennifer-cronin@uiowa.edu
319-335-9917
University of Iowa
30-Mar-1999


Page: 1 2

Related biology news :

1. Laboratory grows world record length carbon nanotube
2. Story tips from the Department of Energys Oak Ridge National Laboratory, September 2004
3. Pacific Northwest National Laboratory gets $10 million from NIH to build virtual lung
4. Story tips from the Department of Energys Oak Ridge National Laboratory, August 2004
5. Story tips from the Department of Energys Oak Ridge National Laboratory, July 2004
6. Oak Ridge National Laboratory to develop DOE leadership-class computing capability for science
7. Institute for Systems Biology, Pacific Northwest National Laboratory announce collaboration
8. Story tips from the Department of Energys Oak Ridge National Laboratory, April 2004
9. Laboratory rat gene sequencing completed
10. ORNL earns four Federal Laboratory Consortium awards
11. Story tips from the Department of Energys Oak Ridge National Laboratory, February 2004

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Laboratory Develops Procedure Study DNA Replication

(Date:4/16/2014)... store memories improves during childhood, associated with structural ... prefrontal and parietal cortices. New research from UC ... at this crucial time. Eventually, that could give ... transition into and during adolescence and affect memory, ... in the middle of the brain, the hippocampus ...
(Date:4/16/2014)... Dr. Klaus Aktories and Dr. Panagiotis Papatheodorou from the ... the University of Freiburg have discovered the receptor responsible ... perfringens into the cell. The TpeL toxin, which ... that causes gas gangrene and food poisoning. It is ... germs of the genus Clostridium . The toxins ...
(Date:4/15/2014)... 15, 2014, Irvine, Calif. UC Irvine neurobiologists have ... results when transplanted into the brains of mice with ... trial is published in the journal Stem Cells ... shown to work in two different mouse models. ... of dementia, is associated with accumulation of the protein ...
Breaking Biology News(10 mins):How kids' brain structures grow as memory develops 2Gate for bacterial toxins found 2UCI study finds modified stem cells offer potential pathway to treat Alzheimer's disease 2
(Date:1/15/2014)... 2014 The Microcompetition with Foreign DNA ... disease. One of these latent viruses is the Epstein ... rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... by the theory, a study found that RA patients ... (1). , What is Microcompetition? , Dr. Hanan ...
(Date:1/15/2014)... Sunnyvale, CA (PRWEB) January 15, 2014 ... of high throughput research solutions, today announced that ... companies, has received delivery of Freeslate’s CM ... form screening. , Lupin, headquartered in Mumbai, ... quality, affordable generic and branded formulations and Active ...
(Date:1/14/2014)... (PRWEB) January 14, 2014 Date: Friday, ... p.m. , Location: Warrington Country Club, 1360 Almshouse Road, ... Foundation, the only national nonprofit organization solely dedicated to ... quality of life for those affected worldwide, will host ... Warrington Country Club in Warrington, Pa. , Each ...
(Date:1/14/2014)... , Jan. 14, 2014  3D Communications, a leading provider ... major scientific, regulatory, business, and media events in ... , today announced its former associate Virginia Cox , ... office. Virginia Cox re-joins ... Commissioner for the Office of External Affairs at the U.S. ...
Breaking Biology Technology:Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3Hepatitis B Foundation to Host Annual Crystal Ball Gala 2Former FDA Associate Commissioner Returns To 3D Communications 2
Cached News: