HOME >> BIOLOGY >> NEWS
UI Laboratory Develops Procedure To Study DNA Replication

IOWA CITY, Iowa -- A University of Iowa research team has developed a way to isolate replicating deoxyribonucleic acid (DNA) molecules that scientists then can use to examine the replication process under controlled conditions. This advance will allow investigators to better understand DNA replication and may lead to improved therapies for treating diseases such as cancer.

"One of the difficulties with studying the DNA replication process is that there are multiple protein complexes involved, and it is difficult to experimentally dissect individual reactions that are occurring," said Marc Wold, Ph.D., UI associate professor of biochemistry and the project's principal investigator. "Our procedure will allow us to examine these reactions in more detail."

DNA is the genetic material that encodes all components in human cells. Each cell contains more than two meters of DNA. Every time a cell divides, it is necessary to duplicate all of its DNA.

Using DNA derived from the Simian Virus 40 from monkeys, Wold and members of his laboratory let DNA replication start in an extract from human cells. The researchers attach magnetic beads to the DNA molecules and use a magnet to isolate the molecules and associated replication proteins. The researchers can then manipulate the extracted DNA and add specific replication proteins in a controlled manner to understand the mechanics of DNA replication.

"This will mean we can start asking specific questions about the reactions during replication and learn more about the specific roles of replication proteins," Wold said.

The advantage of this system is that it allows separation of the DNA replication process from the synthetic reactions. This separation has been difficult to achieve in previous replication systems.

Using his technique, Wold hopes that his lab and others can begin to understand what causes some cells to mutate. DNA replication is a complex process that is very accurate and highly regulate
'"/>

Contact: Jennifer Cronin
jennifer-cronin@uiowa.edu
319-335-9917
University of Iowa
30-Mar-1999


Page: 1 2

Related biology news :

1. Laboratory grows world record length carbon nanotube
2. Story tips from the Department of Energys Oak Ridge National Laboratory, September 2004
3. Pacific Northwest National Laboratory gets $10 million from NIH to build virtual lung
4. Story tips from the Department of Energys Oak Ridge National Laboratory, August 2004
5. Story tips from the Department of Energys Oak Ridge National Laboratory, July 2004
6. Oak Ridge National Laboratory to develop DOE leadership-class computing capability for science
7. Institute for Systems Biology, Pacific Northwest National Laboratory announce collaboration
8. Story tips from the Department of Energys Oak Ridge National Laboratory, April 2004
9. Laboratory rat gene sequencing completed
10. ORNL earns four Federal Laboratory Consortium awards
11. Story tips from the Department of Energys Oak Ridge National Laboratory, February 2004

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/1/2016)... , June 1, 2016 Favorable ... Election Administration and Criminal Identification to Boost Global Biometrics ... recently released TechSci Research report, " Global Biometrics Market ... Competition Forecast and Opportunities, 2011 - 2021", the global ... by 2021, on account of growing security concerns across ...
(Date:5/9/2016)... , UAE, May 9, 2016 ... when it comes to expanding freedom for high net ... Even in today,s globally connected world, there is ... conferencing system could ever duplicate sealing your deal with ... obtaining second passports by taking advantage of citizenship via ...
(Date:4/26/2016)... April 27, 2016 Research ... Multi-modal Biometrics Market 2016-2020"  report to their offering.  ... The analysts forecast the global multimodal ... 15.49% during the period 2016-2020.  Multimodal ... sectors such as the healthcare, BFSI, transportation, automotive, ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... ... 2016 , ... In a new case report published today in STEM CELLS ... developed lymphedema after being treated for breast cancer benefitted from an injection of stem ... with this debilitating, frequent side effect of cancer treatment. , Lymphedema refers ...
(Date:6/23/2016)... Calif. , June 23, 2016  Blueprint Bio, ... biological discoveries to the medical community, has closed its ... Matthew Nunez . "We have received ... with the capital we need to meet our current ... essentially provide us the runway to complete validation on ...
(Date:6/23/2016)... -- On Wednesday, June 22, 2016, the NASDAQ ... Dow Jones Industrial Average edged 0.27% lower to finish at ... Stock-Callers.com has initiated coverage on the following equities: Infinity Pharmaceuticals ... NKTR ), Aralez Pharmaceuticals Inc. (NASDAQ: ARLZ ... Learn more about these stocks by accessing their free trade ...
(Date:6/23/2016)... ... June 23, 2016 , ... Regulatory Compliance ... consulting, provides a free webinar on Performing Quality Investigations: Getting to ... 12pm CT at no charge. , Incomplete investigations are still a major concern ...
Breaking Biology Technology:
Cached News: