HOME >> BIOLOGY >> NEWS
UI Laboratory Develops Procedure To Study DNA Replication

IOWA CITY, Iowa -- A University of Iowa research team has developed a way to isolate replicating deoxyribonucleic acid (DNA) molecules that scientists then can use to examine the replication process under controlled conditions. This advance will allow investigators to better understand DNA replication and may lead to improved therapies for treating diseases such as cancer.

"One of the difficulties with studying the DNA replication process is that there are multiple protein complexes involved, and it is difficult to experimentally dissect individual reactions that are occurring," said Marc Wold, Ph.D., UI associate professor of biochemistry and the project's principal investigator. "Our procedure will allow us to examine these reactions in more detail."

DNA is the genetic material that encodes all components in human cells. Each cell contains more than two meters of DNA. Every time a cell divides, it is necessary to duplicate all of its DNA.

Using DNA derived from the Simian Virus 40 from monkeys, Wold and members of his laboratory let DNA replication start in an extract from human cells. The researchers attach magnetic beads to the DNA molecules and use a magnet to isolate the molecules and associated replication proteins. The researchers can then manipulate the extracted DNA and add specific replication proteins in a controlled manner to understand the mechanics of DNA replication.

"This will mean we can start asking specific questions about the reactions during replication and learn more about the specific roles of replication proteins," Wold said.

The advantage of this system is that it allows separation of the DNA replication process from the synthetic reactions. This separation has been difficult to achieve in previous replication systems.

Using his technique, Wold hopes that his lab and others can begin to understand what causes some cells to mutate. DNA replication is a complex process that is very accurate and highly regulate
'"/>

Contact: Jennifer Cronin
jennifer-cronin@uiowa.edu
319-335-9917
University of Iowa
30-Mar-1999


Page: 1 2

Related biology news :

1. Laboratory grows world record length carbon nanotube
2. Story tips from the Department of Energys Oak Ridge National Laboratory, September 2004
3. Pacific Northwest National Laboratory gets $10 million from NIH to build virtual lung
4. Story tips from the Department of Energys Oak Ridge National Laboratory, August 2004
5. Story tips from the Department of Energys Oak Ridge National Laboratory, July 2004
6. Oak Ridge National Laboratory to develop DOE leadership-class computing capability for science
7. Institute for Systems Biology, Pacific Northwest National Laboratory announce collaboration
8. Story tips from the Department of Energys Oak Ridge National Laboratory, April 2004
9. Laboratory rat gene sequencing completed
10. ORNL earns four Federal Laboratory Consortium awards
11. Story tips from the Department of Energys Oak Ridge National Laboratory, February 2004

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/15/2016)... , March 15, 2016 Yissum ... , the technology-transfer company of the Hebrew University, announced ... of remote sensing technology of various human biological indicators. ... raising $2.0 million from private investors. ... based on the detection of electromagnetic emissions from sweat ...
(Date:3/10/2016)... -- --> --> ... Access Management Market by Component (Provisioning, Directory Services, Password ... Size, by Deployment, by Vertical, and by Region - ... is estimated to grow from USD 7.20 Billion in ... Compound Annual Growth Rate (CAGR) of 12.2% during the ...
(Date:3/8/2016)... 8, 2016   Valencell , the leading ... it has secured $11M in Series D financing. ... new venture fund being launched by UAE-based financial ... existing investors TDF Ventures and WSJ Joshua Fund. ... its triple-digit growth and accelerate its pioneering innovation ...
Breaking Biology News(10 mins):
(Date:5/25/2016)... ... May 25, 2016 , ... ... recently became double board-certified in surgery and surgery of the hand by the ... is no stranger to going above and beyond in his pursuit of providing ...
(Date:5/25/2016)... ... May 25, 2016 , ... WEDI, the nation’s leading authority on ... that Charles W. Stellar has been named by the WEDI Board of Directors as ... As an executive leader with more than 35 years of experience in healthcare, association ...
(Date:5/24/2016)... , ... May 24, 2016 , ... ... diabetes, and traumatic injuries, will be accelerated by research at Worcester Polytechnic Institute ... engines of wound healing and tissue regeneration. , The novel method, developed by ...
(Date:5/24/2016)... ... May 24, 2016 , ... Media Cybernetics, ... new Media Cybernetics corporate branding reflects a results-driven revitalization for a company with ... The re-branding components include a crisp, refreshed logo and a new web presence. ...
Breaking Biology Technology:
Cached News: