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UK scientists develop 'his-and-hers' fertility test kit

ated with colloidal gold and a second monoclonal antibody to a different antigenic determinant, the latter antibody being immobilized as a line on a nitrocellulose strip.

FSH present in the urine reacts with the conjugated antibody and immobilized antibody, forming a sandwich immunocomplex at the site of the immobilized antibody. Unreacted conjugate is washed from the strip by the flow of excess sample. The appearance of a clear red line (test result) indicates the presence of FSH in the urine sample. The intensity of this test line is proportional to the FSH concentration.

The nitrocellulose strip also has a reference line of fixed intensity that is calibrated to the urine concentration corresponding to a serum FSH value of 10 IU/L. A test line of color intensity greater than or equal to the reference line indicates an elevated FSH concentration and diminished ovarian reserve.


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Contact: Margaret Willson
m.willson@mwcommunications.org.uk
41-21-643-33-33
European Society for Human Reproduction and Embryology
2-Jul-2001


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