HOME >> BIOLOGY >> NEWS
Using RNA interference to tune gene activity in stem cells

The application of RNA interference (RNAi) to the study of mammalian biology and disease has the potential to revolutionize biomedical research and speed the development of novel therapeutic strategies.

A series of studies by Greg Hannon at Cold Spring Harbor Laboratory (CSHL) have revealed a great deal of information about the mechanism of RNAi, as well as how RNAi can be adapted for use in medical research. These and other studies led Science to name discoveries concerning RNAi the "Breakthrough of the Year" for 2002 among all of the sciences.

Now, researchers at CSHL have shown that RNAi can be used to set the level of gene activity in stem cells on "low," "medium," or "high."

The new study indicates that stable suppression of deleterious genes by RNAi--in which adult stem cells are isolated, modified ex vivo, and then re-introduced into the affected individual--might be an effective strategy for treating human disease.

The study, published in the February issue of Nature Genetics, focussed on the role of a tumor suppressor gene called p53 in a mouse model of lymphoma.

In the mouse model, forced expression of the Myc oncogene in B-cells causes the mice to develop B-cell lymphomas by 4 to 6 months of age. The scientists, led by Greg Hannon and his CSHL colleague, Scott Lowe, knew that completely deleting the p53 gene causes lymphomas to develop much sooner, and in a more aggressive, highly-invasive form, than lymphomas that develop when the p53 gene is present.

To test the effect of decreasing p53 to particular levels via RNA interference, the scientists reconstituted the blood cells of mice by first irradiating the animals to destroy their endogenous, bone marrow supply of hematopoietic stem cells, and then injected the mice with a fresh supply of hematopoietic stem cells that had been engineered through RNAi to produce low, medium, or high levels of p53.

The study showed that establishing different l
'"/>

Contact: Peter Sherwood
sherwood@cshl.edu
516-367-6947
Cold Spring Harbor Laboratory
2-Feb-2003


Page: 1 2

Related biology news :

1. Using statistics to decipher secrets of natural mutation
2. Using science to restore habitat for declining species
3. Using soils as filters to prevent crypto from moving to the groundwater
4. Using a companion crop to control weeds organically
5. Using plants and microbes to purify polluted industrial wastewater
6. Using GPR to estimate tree root biomass
7. Using computational power tools to bolster systems biology
8. Using sewage sludge as fertilizer
9. Using the oceans living light shows to fight terrorism or track the planets most massive migration
10. Using genetic research to treat inflammatory diseases Vienna centre of excellence in biomedicine harnesses basic research
11. Using brain activity to identify risk for disorders

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:11/6/2019)... ... 06, 2019 , ... NDA Partners Chairman Carl Peck, ... with expertise in clinical pharmacology and pharmacometrics and a widely recognized expert in ... joined the firm as an Expert Consultant. , Dr. Stanski has served in ...
(Date:11/6/2019)... ... November 06, 2019 , ... Genomenon® announced ... now includes the ability to search the entirety of the genomic literature by ... and rare diseases. , Patients, particularly those with rare diseases, don’t always have ...
(Date:11/5/2019)... Seattle, WA (PRWEB) , ... November 05, 2019 ... ... LeCuyer & Amato Dentistry, providing state-of-the-art cosmetic and restorative dentistry to Seattle, WA, ... Receiving her Doctor of Dental Surgery degree from the University of Washington, Dr. ...
Breaking Biology News(10 mins):
(Date:11/19/2019)... ... 2019 , ... Supercomputing Conference – Cirrascale Cloud Services® ... Autonomous Vehicle , Medical Imaging, and Natural Language Processing (NLP) workflows, today announced ... software stack as part of its bare-metal cloud service offerings. In addition to ...
(Date:11/12/2019)... ... 12, 2019 , ... Peggy Lillis Foundation (PLF) has launched ... infection C. diff. The campaign, “See C. diff,” combines basic facts ... 30,000 people in the U.S. each year. The campaign also features a portrait ...
(Date:11/5/2019)... ... November 05, 2019 , ... ... received CE Mark for its Fusion Bx 2.0 prostate fusion biopsy solution. By ... the Fusion Bx 2.0 will give urologists across Europe the ability to perform ...
(Date:10/30/2019)... ... October 30, 2019 , ... While using cold plasma to kill cancer cells ... Dakota School of Mines & Technology are exploring new ways to regulate cold ... , If successful, the technique would prove to be a drug-free, minimally invasive cancer ...
Breaking Biology Technology:
Cached News: