HOME >> BIOLOGY >> NEWS
Wonder Thread: UD Scientists Report First Protein With Collagen And Elastin-Like Domains

take advantage of their remarkable properties, says Harold Slavkin, director of the National Institute of Dental Research, one of the National Institutes of Health, which sponsored the UD study. "Insight into the molecular structure that makes the byssus strong yet flexible might suggest, for example, new strategies for designing more comfortable and pliable artificial skin," Slavkin says.

Curious Forms of Collagen

To find out what makes byssal threads so special, Qin and Waite first isolated two key collagens: Col-P and Col-D. They used pepsin, an enzyme secreted by stomach cells of vertebrates, to pinpoint the collagens. Unlike most proteins, Waite says, Col-P and Col-D don't break apart in response to pepsin. Since pepsin works best in an acidic environment, researchers simply placed byssal threads and pepsin in a weak solution of acetic acid. "Col-P and Col-D were the only proteins detectable after pepsinization," Waite explains. "Within byssal threads, these two collagens are distributed in a complementary gradient, with Col-P predominant in the elastic, proximal region, near the foot of the mussel, and more Col-D at the far, or distal end."

The UD team also examined the protein precursors for Col-P and Col-D, found in the mussel foot, where byssal threads are produced. Specific antibodies--proteins designed to detect and fight off foreign molecules by binding with them--helped Coyne target preCol-P. First, messenger RNA (ribonucleic acid) containing the genetic code for the protein was extracted from mussel foot tissue. Next, RNA was converted to the more stable DNA (deoxyribonucleic acid) form and cloned into bacteria, which expressed the protein encoded by the mussel's RNA. Designer antibodies, produced by a laboratory animal injected with Col-P, quickly latched onto the protein precursor expressed by bacterial clones.

Three Major Domains

The preCol-P in byssal threads contains three major domains, Coyne says. The middle section
'"/>

Contact: Ginger Pinholster
ginger.pinholster@mvs.udel.edu
302-831-6408
University of Delaware
18-Sep-1997


Page: 1 2 3 4

Related biology news :

1. Wonderfest science festival returns to Stanford Nov. 2-3
2. New "Wonder Peanut" Beats Olive Oil In Healthful Benefits
3. Scientists to prototype cyberinfrastructure for research and education access to ocean observatories
4. Scientists sequence genome of kind of organism central to biospheres carbon cycle
5. Scientists find nanowires capable of detecting individual viruses
6. Scientists discover potential new way to control drug-resistant bacteria
7. Scientists explore genome of methane-breathing microbe
8. Scientists decipher genetic code of biothreat pathogen
9. Stuck on you: Scientists lay bare secrets of bacterial attachment proteins
10. Scientists discover proteins involved in spread of HIV-1 infection
11. Scientists fear new Ebola outbreak may explain sudden gorilla disappearance

Post Your Comments:
(Date:7/23/2014)... Tracking of blue whales by satellite over a 15-year ... whales consistently return to feed in specific locations each ... in the open-access journal PLOS ONE by ... data may be used to mitigate human threats to ... has been slow to recover since the establishment of ...
(Date:7/23/2014)... cultured from cystic fibrosis patients, scientists at the UNC ... have shown that a new CF drug counteracts the ... The finding, published today in the journal Science ... becomes unstable and loses its ability to function properly ... research offers several insights into how novel CF pharmacotherapies ...
(Date:7/23/2014)... , , , , , ... , , , , , ... that changing how medical care is delivered could help prevent multiple chronic diseases and extend healthy lifespan. The idea, they argue, would be to target the... ... , , , , ... , , , , , ...
Breaking Biology News(10 mins):Blue whales' dangerous feeding grounds 2UNC researchers find unsuspected characteristics of new CF drugs, offering potential paths to more effective therapies 2UNC researchers find unsuspected characteristics of new CF drugs, offering potential paths to more effective therapies 3UNC researchers find unsuspected characteristics of new CF drugs, offering potential paths to more effective therapies 4Strategy proposed for preventing diseases of aging 2Strategy proposed for preventing diseases of aging 3Strategy proposed for preventing diseases of aging 4Strategy proposed for preventing diseases of aging 5
(Date:7/23/2014)... /PRNewswire-iReach/ -- Think Drinks are a neuroscience-backed drink regimen ... They are the first functional focus beverage to give ... alternative to the temporary fix associated with energy drinks. ... 29, 2014, with hopes to raise $50,000 over the ... http://photos.prnewswire.com/prnh/20140723/129697 Think Drinks were ...
(Date:7/23/2014)... 23 de julio de 2014 ADB ... Compañía) ha anunciado hoy una asociación con Austen ... (ABIA o el Instituto), para el propósito de ... de terapia de señal bioeléctrica (el E-QURE BST ... Instituto, que tiene una importante experiencia en cicatrización ...
(Date:7/23/2014)... , July 23, 2014 regulated information -- UCB ... and development pipeline with positive topline results from ... . This study was designed to evaluate the efficacy ... 200 mg/day, without titration) compared to placebo, as ... partial-onset seizures, not fully controlled despite treatment with ...
(Date:7/23/2014)... 23, 2014 With the ability to record ... for more than 15 minutes, Fastec Imaging has brought the ... high speed imaging. “Finally, a high speed camera that can ... in our everyday lives.” proclaims Matt Kearney, VP of Sales ... been to demystify and simplify the traditionally complicated workflow of ...
Breaking Biology Technology:truBrain Neuroscientists To Launch More Effective, Healthier Alternative To Energy Drinks On Indiegogo 2ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 2ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 3ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 4ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 5ADB International Group Inc. anuncia una asociación con Austen BioInnovation Institute 6Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 2Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 3Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 4Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 5Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 6Major advance in UCB pipeline: positive topline Phase 3 results for brivaracetam in epilepsy patients with partial-onset seizures 7Fastec Imaging Introduces the First High Speed Camcorder 2Fastec Imaging Introduces the First High Speed Camcorder 3
Cached News: