HOME >> BIOLOGY >> NEWS
Yale scientists visualize details of how hepatitis C unwinds RNA

New Haven, Conn. -- Research led by Anna Marie Pyle, professor of molecular biophysics and biochemistry at Yale University reveals how a protein from Hepatitis C (HCV) unwinds RNA, potentially allowing it to be copied.

The work published in the journal Nature focuses on an enzyme, helicase NS3, that unwinds the RNA virus for replication inside cells. NS3 is one member of an extensive family of helicases and is used as a model for studying unwinding activities of motor proteins.

Their findings are particularly important because NS3 is a major drug target against HCV and understanding the helicase function will aid in the development of HCV inhibitors.

Pyle's results contradict the idea that helicases move smoothly with the continuous action of a snow plow. Instead, NS3 moves with a discontinuous stepping motion that alternates rapid translocation with pausing. "We observe that the helicase proceeds through discreet spatial and kinetic microstates," Pyle said. "We actually track the speed and processivity of the helicase as it passes through each base pair of its substrate."

"While this report is the first of its kind, and has produced highly significant results, it is only the beginning of a new understanding in HCV enzymology," said Pyle. "In the future, our approaches will be used to understand the composition of the HCV replication complex and the interplay between its constituent proteins. Comparative studies will be done on other viruses and in other systems where helicase function is critical."

These novel features were revealed using a new type of combinatorial enzymology that allows the behavior of helicase enzymes to be directly compared on a sequence panel. This is the first time that the behavior of a nucleic acid remodeling protein has been monitored at this high resolution, as it acts upon or passes each subunit of its target, according to Pyle. Since the work was conducted on RNA, it helps to b
'"/>

Contact: Janet Rettig Emanuel
janet.emanuel@yale.edu
203- 432-2157
Yale University
23-Jul-2004


Page: 1 2

Related biology news :

1. DNA lends scientists a hand, revealing new chemical reactions
2. Conference at UH opens doors for new scientists, engineers
3. Wisconsin scientists develop quick botox test
4. UCI scientists successfully target key HIV protein; breakthrough may lead to new drug therapies
5. Alaska scientists find Arctic tundra yields surprising carbon loss
6. UAF scientists discover new marine habitat in Alaska
7. Information system to help scientists analyze mechanisms of social behavior
8. Zoonotic diseases - European scientists unite to fight diseases
9. Israeli scientists reveal the plan of a key cellular machine
10. Study by Israeli scientists provides insight on DNA code
11. Computer scientists at UH developing nurturing computers

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/10/2016)... March 10, 2016   Unisys Corporation (NYSE: ... Protection (CBP) is testing its biometric identity solution at ... to help identify certain non-U.S. citizens leaving the ... test, designed to help determine the efficiency and accuracy of ... February and will run until May 2016. --> ...
(Date:3/9/2016)... BEACH GARDENS, Fla. , March 9, 2016 ... identity management authentication and enrollment solutions, today announced ... DigitalPersona ® Altus multi-factor authentication ... IT and InfoSec managers to step-up security where ... Washington, DC . ...
(Date:3/3/2016)... , March 3, 2016  2016FLEX, organized by ... week highlighting advancements in flexible, hybrid and printed ... setting attendance - have gathered for short courses, ... field of electronics. The Flex Conference celebrates its ... for companies, R&D organizations, and universities contributing to ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... May 26, 2016 Q BioMed Inc. ... will be a featured presenter at the 5th Annual Marcum ... New York City at the Grand Hyatt Hotel. ... Q BioMed Inc. CEO, is scheduled to begin at 11a.m ... company,s business strategy, recent developments and outline milestones for the ...
(Date:5/25/2016)... ... May 25, 2016 , ... Lajollacooks4u has become a rising hotspot for ... one of its top attractions. Fortune 500 companies, such as Illumina, Hewlett-Packard, Qualcomm ... and intimate team-building experience. , Each event kicks off with an olive oil and ...
(Date:5/25/2016)... ... ... at the University of Athens say they have evidence that the variety of different ... lead to one good one. Surviving Mesothelioma has just posted an article on the ... 98 mesothelioma patients who got a second kind of drug therapy after ...
(Date:5/24/2016)... 24, 2016   MedyMatch Technology Ltd ., the data ... real-time decision support tools in the emergency room, announced today ... Israeli Advanced Technology Industries (IATI) BioMed Conference. ... 15th National Life Sciences and Technology Week, and is ... Hotel in Tel Aviv, Israel . ...
Breaking Biology Technology:
Cached News: