HOME >> BIOLOGY >> NEWS
A rainbow of methods promises insights into biological processes and diseases

COLD SPRING HARBOR, N.Y. (Tues., May 1, 2007) Physical alterations of DNA in chromosomes can cause serious diseases such as Down syndrome, Prader-Willi syndrome, or cancer. Likewise, changes to the physiological environment of cellswith drugs or toxins, for examplecan alter their metabolic output. To track these processes, scientists need ways to mark genes and their protein products. This month's release of Cold Spring Harbor Protocols (www.cshprotocols.org) features freely available methods for marking molecules to identify gene alterations and metabolic shifts.

The first method (http://www.cshprotocols.org/cgi/content/full/2007/10/pdb.prot4743) describes how to grow cells from bacteria, yeast, insects, or mammals in media containing the stable isotope nitrogen-15. As the cells grow, they produce nascent proteins that include nitrogen-15, a marker that distinguishes newly formed metabolic products from pre-existing ones. The differences between these cells and "normal" cells (grown in media without nitrogen-15) can then be quantified using a technique called mass spectrometry.

To identify structural features and abnormalities in chromosomes, scientists need to tag DNA probes with visual markers. The second freely available method (http://www.cshprotocols.org/cgi/content/full/2007/10/pdb.prot4730) describes how to label DNA probes with fluorescent markers of six different colors, which span the visible spectrum. These labeled DNA probes can be used simultaneously in FISH (fluorescent in situ hybridization) experiments, during which the probes bind to specific chromosomal regions of interest. The colorful chromosomes can then be viewed under a microscope.


'"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
1-May-2007


Page: 1

Related biology news :

1. Hearts of male and female rainbow trout are different
2. Bioinformatics-related methods are featured in Cold Spring Harbor Protocols
3. Rutgers, Penn researchers capture research dollars to improve prostate cancer diagnostic methods
4. Cold Spring Harbor Protocols features methods to understand embryos, clones, stem cells
5. New methods and tools needed to measure exposure to airborne nanomaterials
6. Cold Spring Harbor Protocols highlights reliable methods for gene and protein analyses
7. Application of Genomatix in silico methods reveals novel cancer associated genes
8. A frenzy of fruit fly methods featured in Cold Spring Harbor Protocols
9. Plant-derived molecules, genetic manipulation point to future chemoprevention methods
10. Cells, dyes and videotape: Online scientific methods journal incorporates multimedia
11. CSH Protocols publishes cutting-edge methods for analyzing complex molecular interactions

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/21/2020)... ... February 21, 2020 , ... Gateway Genomics , a leading developer of ... last year’s annual Inc. 5000 list of fastest-growing private companies in the ... to be the 17th fastest-growing private company in the state, and #1 in the ...
(Date:2/13/2020)... ... February 13, 2020 , ... Strategic Directions International (SDi) announced ... & Life Science Instrumentation Industry. The report will be released on February 28th. ... for the $70 billion laboratory market for analytical and life science instruments. Special ...
(Date:2/11/2020)... HOUSTON (PRWEB) , ... February 11, 2020 , ... ... engineering firm, is pleased to announce it achieved its ISO 9001:2015 registration by ... In preparation for the audits, she reviewed and revised all existing Standard Operating ...
Breaking Biology News(10 mins):
(Date:2/10/2020)... VEGAS (PRWEB) , ... February 10, 2020 , ... R3 ... Training Course March 13-14th, 2020 in Las Vegas. The course is hands on ... too. , For the past few years, the Comprehensive Stem Cell Training Course has ...
(Date:1/28/2020)... ... January 28, 2020 , ... ... distributor to food and beverage manufactures within North America. Both Lifecycle and Midland ... agreement will allow each company to pool their collective resources and provide the ...
(Date:1/24/2020)... ... January 24, 2020 , ... The plastic and reconstructive surgeons at The ... epitomize the notion that plastic surgery is much more than just cosmetic surgery and ... Over the last 20 years NJ Top Docs, Dr. Andrew Elkwood, Dr. Michael Rose, ...
(Date:1/22/2020)... ... January 21, 2020 , ... Microbial Discovery Group LLC (MDG) ... Industrial and Institutional (I&I) Account Manager. Darrell will focus on servicing MDG’s partners ... over 30 years of sales experience working across multiple different market sectors such ...
Breaking Biology Technology:
Cached News: