Every time a cell divides, the protective envelope that surrounds the nucleus is broken down and rebuilt into two new ones. Envelopes are highly complex structures of membranes and proteins which must be precisely reassembled for the nuclei to function. Scientists at the Institute for Research in Biomedicine (IRB) in Barcelona, the European Molecular Biology Laboratory (EMBL) in Heidelberg and the Pasteur Institute in Paris have discovered a protein that plays a crucial role in the assembly and structure of the nucleus. Their work appears in the September 5 issue of Current Biology.
The envelope acts as a barrier between the outer cell compartment, called the cytoplasm, and DNA stored in the cell nucleus. It regulates which molecules are allowed to pass back and forth between the two compartments. Most of this traffic passes through basket-shaped passageways called nuclear pores, which consist of intricately-woven proteins. "We haven't yet identified all the molecules in the nuclear envelope, and many questions remain about the process by which molecules are granted or denied passage," says Peter Askjaer of IRB.
The new study shows that a protein called MEL-28 is a component of nuclear pores in the worm C. elegans, one of biology's most important model organisms. More importantly, it reveals that MEL-28 is one of the key architects as bits of membrane and proteins are drawn together to build new envelopes.
When scientists blocked the activity of MEL-28, they discovered that patches of membranes attached themselves to DNA but couldn't seal themselves off into a complete envelope. A step-by-step analysis showed that without the protein, other molecules are not drawn together properly as envelopes are rebuilt. The components were scrambled; pores were no longer built, and the wrong molecules were able to get access to DNA. Because MEL-28 remains attached to DNA during the entire process of cell division, the scientists believe it plays a c
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Contact: Sarah Sherwood
ssherwood@pcb.ub.es
34-934-037-255
Institute for Research in Biomedicine (IRB)
5-Sep-2006