HOME >> BIOLOGY >> NEWS
Berkeley scientists get first detailed look at Dicer

Dicer enzyme in Giardia is identical to the core of a Dicer enzyme in higher eukaryotes, including humans, that cleaves dsRNA into lengths of about 25 bases.

In their Science paper, Doudna and her colleagues describe a front view of the structure as looking like an axe. On the handle end there is a domain that is known to bind to small RNA products, and on the blade end there is a domain that is able to cleave RNA. Between the clamp and the cleaver is a flat-surfaced region that carries a positive electrical charge. Doudna and her colleagues propose that this flat region binds to the negatively charged dsRNA like biological Velcro, enabling Dicer to measure out and snip specified lengths of siRNA.

"When you put the clamp, the flat area and the cleaver together, you get a pretty good idea as to how Dicer works," Doudna said. "We're now using this structural model to design experiments that might tell us what triggers Dicer into action."

Different forms of the Dicer enzyme are known to produce different lengths of siRNA. Having identified the flat-surfaced positively charged region in Dicer as the "ruler" portion of the enzyme, it may be possible to alter the length of a long connector helix within this domain to change the lengths of the resulting siRNA products.

"One size does not fit all for Dicer, it makes dsRNA products that range from 21 to 30 base pairs in length or longer. We would like to see what happens when you take a natural Dicer and change the length of its helix," Doudna said.

Determining Dicer's crystal structure was made possible through the unique crystallography capabilities of ALS Beamline 8.2.1 and 8.2.2, Doudna said. Funded through HHMI, beamlines 8.2.1 and 8.2.2 are powered by a superconducting bend magnet, an ideal source of x-rays for protein crystallography experiments. The "superbend" magnet is used to extract x-rays from a relativistic beam of electrons circulating through the ALS stora
'"/>

Contact: Lynn Yarris
lcyarris@lbl.gov
510-486-5375
DOE/Lawrence Berkeley National Laboratory
12-Jan-2006


Page: 1 2 3 4

Related biology news :

1. Berkeley Lab Life Sciences division awarded NIH grants for fruit fly, nematode studies
2. Lab-on-a-chip device from Berkeley Lab to speed proteomics research
3. Berkeley Cancer Genome Center to study tumor genomics
4. Berkeley lab life scientists are leaders in team to detect cancer by studying proteins in the blood
5. NIH funds Berkeley Lab research on defense against radiological attack
6. DDT in mothers linked to developmental delays in children, UC Berkeley study finds
7. At Berkeley: Intelligently designed molecular evolution
8. Berkeley researchers lay groundwork for cell version of DNA chip
9. ORNL, UC Berkeley unravel real-world clues to Earths mysteries
10. Octopuses occasionally stroll around on two arms, UC Berkeley biologists report
11. Wolves alleviate impact of climate change on food supply, finds new UC Berkeley study

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/31/2019)... ... August 30, 2019 , ... Today marks the introduction ... consumers in the preconception period with doctor-approved products and resources, including The Get ... are trying to get pregnant. , Natalist is built on the values of ...
(Date:8/29/2019)... ... August 28, 2019 , ... Salimetrics, ... passed their five-year milestone of collaboration in salivary bioscience stress research. ... primary focus towards performing feasibility studies, phase 1 – phase 4 clinical ...
(Date:8/27/2019)... ... August 27, 2019 , ... Ambry Genetics ... improve genetic testing guidelines. In the largest cohort study of its kind, Ambry ... at-risk patients. , Clinicians use guidelines to inform decisions about which patients ...
Breaking Biology News(10 mins):
(Date:8/15/2019)... ... August 15, 2019 , ... PathSensors ... Lawrence is a member of the Institute of Food Technologists (IFT) Board of ... Advisory Board, Joint Institute for Food Safety and Applied Nutrition (JIFSAN) Advisory Council ...
(Date:8/8/2019)... ... August 08, 2019 , ... Inference Solutions , ... organizations, today announced that the company’s Studio Intelligent Virtual Agent (IVA) platform earned ... Inference will officially accept the award at Frost & Sullivan’s annual Excellence in ...
(Date:8/6/2019)... (PRWEB) , ... August 06, 2019 , ... ... tools for characterizing microbiome populations down to the strain level, has announced partnerships ... kits and other products to companies and research organizations in Australia, Benelux, Indonesia, ...
(Date:8/4/2019)... ... August 02, 2019 , ... Cirtec Medical Corporation, a strategic outsourcing partner for ... has begun construction on a 30,000 sq. ft. manufacturing facility in the Coyol ... , The facility, which is expected to be fully operational by the end of ...
Breaking Biology Technology:
Cached News: