HOME >> BIOLOGY >> NEWS
Bioengineering efficient antibiotic biosynthesis in E. coli

The pathways underlying the production of antibiotics are now quite well known. For example, the antibacterial activity of erythromycin, an important polyketide antibiotic precursor, requires the transfer of two unusual sugars called mycarose and desosamine (both glycosyl groups) onto the nonsugar part of the glycoside molecule (macrocyclic aglycone).

In a new study published online this week in the open-access journal PLoS Biology, Ho Young Lee and Chaitan Khosla demonstrate how they used bioassay-guided evolution of this antibiotic pathway in Escherichia coli (E. coli) to identify more efficient antibiotic-producing mutants.

The authors reconstituted the biosynthetic pathways of both sugars in E. coli to yield the 6-deoxyerythromycin D antibiotic. By engineering a recombinant strain of E. coli that produces the bioactive macrolide 6-deoxyerythromycin D from propionate, they developed a fundamentally new tool for enhancing the efficiency of biosynthetic engineering of this class of antibiotics. Initially, this recombinant strain produced barely enough antibiotic activity to establish an activity-based screening assay. The authors therefore used the assay to screen for antibiotic overproducers. After three rounds of screening, they were able to identify E. coli cells that overproduced the 6-deoxyerythromycin D antibiotic with significant modifications in the mycarose biosynthetic pathway. They used the same activity-based screening system to evolve E. coli mutants capable of more efficient precursor-directed biosynthesis. As the first example of bioassay-guided evolution of an antibiotic pathway in E. coli, these results open the door for harnessing the power of genetics for mechanistic investigations into polyketide synthases and also for biosynthetic engineering.


'"/>

Contact: Natalie Bouaravong
press@plos.org
415-568-3445
Public Library of Science
5-Feb-2007


Page: 1

Related biology news :

1. Computer model maps efficient inoculation of hospital staff in pandemic outbreak
2. Study: Long legs are more efficient
3. A more powerful and efficient engine for rice: the C3-C4 challenge
4. Celiac success: New enzyme efficiently degrades gluten in human stomach environment
5. New technology enables faster, more efficient cell harvest: Cell therapy meeting study
6. Killing wolves may not protect livestock efficiently
7. Inefficient immune killer cells abet HIV infection
8. Mispairs in genetic material make protein synthesis more efficient
9. New technique points to safer, more efficient vaccination
10. New study shows Emezine demonstrates more efficient absorption
11. Getting old? Slowing down? Blame inefficient mitochondria

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/27/2021)... ... March 25, 2021 , ... Phlexglobal announced ... Planet Group, has selected Phlexglobal and its innovative regulatory SaaS software, PhlexSubmission, as ... a comprehensive review of five regulatory software companies, with the review team including ...
(Date:3/27/2021)... ... 25, 2021 , ... The 2021 Virtual Conference on Clinical Trial Supply-Europe ... More and more, clinical trial supply conferences are featuring speakers and forums that ... Asymmetrex’s founder and CEO, James L. Sherley, M.D., Ph.D., presented a talk ...
(Date:3/23/2021)... ... March 23, 2021 , ... G-CON Manufacturing (G-CON), the ... by Matica Biotechnology (Matica Bio), a contract development and manufacturing organization (CDMO) specializing ... the cleanroom build out for its new GMP production facility in College Station, ...
Breaking Biology News(10 mins):
(Date:3/27/2021)... ... March 24, 2021 , ... ... Life Sciences and Healthcare firms of all sizes, adds depth to its team ... specialist. Pardillo, who earned his doctorate in computational chemistry from Florida International University ...
(Date:3/27/2021)... ... March 24, 2021 , ... ABI Wellness, ... and reporting approach designed under CEO Mark Watson, today announced a webinar dedicated ... featuring guest speakers Dr. Cameron Clark, Neuropsychologist and Founder of Sharp Thinking, and ...
(Date:3/27/2021)... ... ... The Xtalks editorial team is pleased to announce the launch of the ... joined by editorial team members Ayesha Rashid, Sydney Perelmutter and Mira Nabulsi to discuss ... including insights from industry experts. , The Xtalks Life Science podcast will feature ...
(Date:3/23/2021)... Conn. (PRWEB) , ... March 23, 2021 , ... ... develops solutions for characterizing microbiome populations down to the strain level, recently unveiled ... applications. , Not all microbes are created equal: some are easy to ...
Breaking Biology Technology:
Cached News: