HOME >> BIOLOGY >> NEWS
Bioluminescence at the service of a novel cerebral imaging technique

CNRS scientists in collaboration have developed a new technique for the in vivo imaging of neuronal function using bioluminescence, based on a GFP-aequorin fusion protein. This imaging technique enables the monitoring of neuronal activity (and more specifically, calcium activity), real-time and in-vivo, in either a small group of neurons or in the brain as a whole.

Participating in the development were The Molecular Embryology Unit (CNRS/Institut Pasteur) in collaboration with the Cellular and Molecular Neurobiology Laboratory (CNRS) and the Neurobiology Laboratory for Learning, Memory and Communication (CNRS/Universit Paris-Sud).

The novel imaging technique employs a new, GFP-aequorin marker/tracer. This is a calcium-sensitive protein, which in the presence of its co-factor , coelenterazine, will emit light (a photon) when there is a change to the calcium concentration in a cell; for example, following neuronal activation. This makes it possible to follow neuronal activity in neurons, or even to trace it in a network of neurons. Furthermore, this little-invasive and non-toxic approach allows the recording of neuronal activity over periods of several hours. It is thus possible to monitor the cerebral activity of a Drosophila fruit fly for 24 or even 48 hours.

Because of these characteristics, the new tracer can demonstrate new physiological phenomena related to calcium activity. Thus the activation by nicotine of pedunculate bodies (an important structure for learning and olfactory memory in the Drosophila) induces a secondary response which is delayed by about 10 to 15 minutes at the level of neuronal axonal projections. It is therefore probable that this new response (hitherto totally unsuspected) intervenes in learning and memory phenomena.

Furthermore, using this imaging technique, it has been possible to record neurons in the ellipsoid body, a structure involved in regulating locomotor activity. This stru
'"/>

Contact: Monica McCarthy
monica.mccarthy@cnrs-dir.fr
33-144-965-191
CNRS
17-Apr-2007


Page: 1 2 3

Related biology news :

1. How is the digitization of information impacting across the health service?
2. AACR to present distinguished public service awards at 2007 Annual Meeting
3. NIH neuroscience microarray consortium launches high-throughput genotyping services
4. St. Josephs provides tissue processing services to researchers in hopes of finding a cure
5. Assessing ecosystem services to identify conservation priorities
6. Biodiversity controls ecological services, report scientists in comprehenisive analysis
7. Carbon capture, water filtration, other boreal forest ecoservices worth estimated $250 billion/year
8. ACS news service Weekly PressPac -- August 16, 2006
9. Ecosystem services and invasive species
10. Forest service researchers test Chinese tallow tree for use in building materials
11. ACS news service weekly press package - May 15, 2006

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/7/2017)... Ind. , Feb. 7, 2017 Zimmer ... leader in musculoskeletal healthcare, will present at the LEERINK ... New York Palace Hotel on Wednesday, February 15, 2017 ... live webcast of the presentation can be accessed at ... replay following the conference via Zimmer Biomet,s Investor Relations ...
(Date:2/6/2017)... According to Acuity Market Intelligence, ongoing ... to continue to embrace biometric and digital identification ... Border Control (ABC) eGates and 1436 Automated Passport ... 163 ports of entry across the globe. Deployments ... combined CAGR of 37%. APC Kiosks reached 75% ...
(Date:2/3/2017)... A new independent identity strategy consultancy firm ... . Designed to fill a critical niche in technical ... partners Mark Crego and Janice Kephart ... identity expertise that span federal governments, the 9/11 Commission, ... combined expertise has a common theme born from a ...
Breaking Biology News(10 mins):
(Date:2/23/2017)... ... February 22, 2017 , ... ... , have been named Fellows of the Society this year, the Fellows Committee ... the multidisciplinary fields of optics, photonics, and imaging as well as their service ...
(Date:2/22/2017)... , Feb. 22, 2017  PrimeVax Immuno-Oncology, Inc. announced ... presenting at the Annual Biocom Global Life Science Partnering ... at 11:15 AM, at the Torrey Pines Lodge, in ... the organizers at Biocom who have chosen our company, ... of biotechnology companies, investors, and clinical researchers," said Mr. ...
(Date:2/22/2017)... ... February 22, 2017 , ... ... and scientists from around the world, is pleased to announce the 2nd annual ... 23, 2017. This premier, online-only conference focused on the development and advancements in ...
(Date:2/22/2017)... DIEGO , Feb. 22, 2017 Aethlon ... the results of a study that validated the ability ... that are associated with increased mortality in immune-suppressed sepsis ... patients. The objective of the study ... of Cytomegalovirus (CMV), Epstein-Barr virus (EBV) and Herpes Simplex ...
Breaking Biology Technology:
Cached News: