HOME >> BIOLOGY >> NEWS
Bioluminescence at the service of a novel cerebral imaging technique

CNRS scientists in collaboration have developed a new technique for the in vivo imaging of neuronal function using bioluminescence, based on a GFP-aequorin fusion protein. This imaging technique enables the monitoring of neuronal activity (and more specifically, calcium activity), real-time and in-vivo, in either a small group of neurons or in the brain as a whole.

Participating in the development were The Molecular Embryology Unit (CNRS/Institut Pasteur) in collaboration with the Cellular and Molecular Neurobiology Laboratory (CNRS) and the Neurobiology Laboratory for Learning, Memory and Communication (CNRS/Universit Paris-Sud).

The novel imaging technique employs a new, GFP-aequorin marker/tracer. This is a calcium-sensitive protein, which in the presence of its co-factor , coelenterazine, will emit light (a photon) when there is a change to the calcium concentration in a cell; for example, following neuronal activation. This makes it possible to follow neuronal activity in neurons, or even to trace it in a network of neurons. Furthermore, this little-invasive and non-toxic approach allows the recording of neuronal activity over periods of several hours. It is thus possible to monitor the cerebral activity of a Drosophila fruit fly for 24 or even 48 hours.

Because of these characteristics, the new tracer can demonstrate new physiological phenomena related to calcium activity. Thus the activation by nicotine of pedunculate bodies (an important structure for learning and olfactory memory in the Drosophila) induces a secondary response which is delayed by about 10 to 15 minutes at the level of neuronal axonal projections. It is therefore probable that this new response (hitherto totally unsuspected) intervenes in learning and memory phenomena.

Furthermore, using this imaging technique, it has been possible to record neurons in the ellipsoid body, a structure involved in regulating locomotor activity. This stru
'"/>

Contact: Monica McCarthy
monica.mccarthy@cnrs-dir.fr
33-144-965-191
CNRS
17-Apr-2007


Page: 1 2 3

Related biology news :

1. How is the digitization of information impacting across the health service?
2. AACR to present distinguished public service awards at 2007 Annual Meeting
3. NIH neuroscience microarray consortium launches high-throughput genotyping services
4. St. Josephs provides tissue processing services to researchers in hopes of finding a cure
5. Assessing ecosystem services to identify conservation priorities
6. Biodiversity controls ecological services, report scientists in comprehenisive analysis
7. Carbon capture, water filtration, other boreal forest ecoservices worth estimated $250 billion/year
8. ACS news service Weekly PressPac -- August 16, 2006
9. Ecosystem services and invasive species
10. Forest service researchers test Chinese tallow tree for use in building materials
11. ACS news service weekly press package - May 15, 2006

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/18/2016)... , March 18, 2016 ... Suppliers of Biometrics, ICT, Manned & Unmanned Vehicles, Physical infrastructure ... & security companies in the border security market and the ... and Europe has led visiongain ... companies improved success. --> defence & security ...
(Date:3/15/2016)... March 15, 2016 --> ... by Transparency Market Research "Digital Door Lock Systems Market - ... - 2023," the global digital door lock systems market in ... 2014 and is forecast to grow at a CAGR of ... small and medium enterprises (MSMEs) across the world and high ...
(Date:3/14/2016)... NXTD ) ("NXT-ID" or the "Company"), a company ... of a new series of commercials on Time Warner Cable ... .  The commercials will air on Bloomberg TV, Fox Business ... show. --> NXTD ) ("NXT-ID" or the "Company"), ... the airing of a new series of commercials on Time ...
Breaking Biology News(10 mins):
(Date:5/19/2016)... - I dati saranno presentati ... 52 ° Congresso della Società ... - Le conclusioni dello studio indicano un tasso di ... cui il 90% presenta una d urata della risposta ... settantadue per cento dei pazienti ha riscontrato un beneficio clinico. ...
(Date:5/18/2016)... ... May 18, 2016 , ... Shimadzu ... at The University of Toledo. This two-day camp will take place annually starting ... the field of pharmaceutical sciences in preparation for a university academic program. ...
(Date:5/18/2016)... ... May 18, 2016 , ... STACS DNA Inc., the sample tracking software company, ... sexual assault kit processing to help them save time and reduce errors. , Sexual ... to be processed and victims informed of results. Due to a previous lack of ...
(Date:5/18/2016)... ... May 18, 2016 , ... The Academy of Model Aeronautics has ... dream of reaching a total of $1 million in awarded scholarships. , The AMA ... school graduates from across the nation has helped bring the total of AMA scholarships ...
Breaking Biology Technology:
Cached News: