HOME >> BIOLOGY >> NEWS
Bioluminescence at the service of a novel cerebral imaging technique

CNRS scientists in collaboration have developed a new technique for the in vivo imaging of neuronal function using bioluminescence, based on a GFP-aequorin fusion protein. This imaging technique enables the monitoring of neuronal activity (and more specifically, calcium activity), real-time and in-vivo, in either a small group of neurons or in the brain as a whole.

Participating in the development were The Molecular Embryology Unit (CNRS/Institut Pasteur) in collaboration with the Cellular and Molecular Neurobiology Laboratory (CNRS) and the Neurobiology Laboratory for Learning, Memory and Communication (CNRS/Universit Paris-Sud).

The novel imaging technique employs a new, GFP-aequorin marker/tracer. This is a calcium-sensitive protein, which in the presence of its co-factor , coelenterazine, will emit light (a photon) when there is a change to the calcium concentration in a cell; for example, following neuronal activation. This makes it possible to follow neuronal activity in neurons, or even to trace it in a network of neurons. Furthermore, this little-invasive and non-toxic approach allows the recording of neuronal activity over periods of several hours. It is thus possible to monitor the cerebral activity of a Drosophila fruit fly for 24 or even 48 hours.

Because of these characteristics, the new tracer can demonstrate new physiological phenomena related to calcium activity. Thus the activation by nicotine of pedunculate bodies (an important structure for learning and olfactory memory in the Drosophila) induces a secondary response which is delayed by about 10 to 15 minutes at the level of neuronal axonal projections. It is therefore probable that this new response (hitherto totally unsuspected) intervenes in learning and memory phenomena.

Furthermore, using this imaging technique, it has been possible to record neurons in the ellipsoid body, a structure involved in regulating locomotor activity. This stru
'"/>

Contact: Monica McCarthy
monica.mccarthy@cnrs-dir.fr
33-144-965-191
CNRS
17-Apr-2007


Page: 1 2 3

Related biology news :

1. How is the digitization of information impacting across the health service?
2. AACR to present distinguished public service awards at 2007 Annual Meeting
3. NIH neuroscience microarray consortium launches high-throughput genotyping services
4. St. Josephs provides tissue processing services to researchers in hopes of finding a cure
5. Assessing ecosystem services to identify conservation priorities
6. Biodiversity controls ecological services, report scientists in comprehenisive analysis
7. Carbon capture, water filtration, other boreal forest ecoservices worth estimated $250 billion/year
8. ACS news service Weekly PressPac -- August 16, 2006
9. Ecosystem services and invasive species
10. Forest service researchers test Chinese tallow tree for use in building materials
11. ACS news service weekly press package - May 15, 2006

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/21/2016)... , June 21, 2016 NuData ... the new role of principal product architect and ... the director of customer development. Both will report ... technical officer. The moves reflect NuData,s strategic growth ... response to high customer demand and customer focus ...
(Date:6/9/2016)... , June 9, 2016  Perkotek an innovation leader in attendance control systems is ... log work hours, for employers to make sure the right employees are actually signing ... http://photos.prnewswire.com/prnh/20160609/377486LOGO ... ... ...
(Date:6/2/2016)... , June 2, 2016 The Department ... has awarded the 44 million US Dollar project, for the ... Vehicle Plates including Personalization, Enrolment, and IT Infrastructure , ... in the production and implementation of Identity Management Solutions. Numerous ... however Decatur was selected for the ...
Breaking Biology News(10 mins):
(Date:6/23/2016)... Durham, NC (PRWEB) , ... June 23, 2016 , ... ... Odense University Hospital in Denmark detail how a patient who developed lymphedema after being ... (fat) tissue. The results could change the paradigm for dealing with this debilitating, frequent ...
(Date:6/23/2016)... , June 23, 2016 ... Hematology Review, 2016;12(1):22-8 http://doi.org/10.17925/OHR.2016.12.01.22 ... , the peer-reviewed journal from touchONCOLOGY, Andrew ... escalating cost of cancer care is placing an ... result of expensive biologic therapies. With the patents ...
(Date:6/23/2016)... (PRWEB) , ... June 23, 2016 , ... ... on quality, regulatory and technical consulting, provides a free webinar on ... on July 13, 2016 at 12pm CT at no charge. , Incomplete investigations ...
(Date:6/23/2016)... PUNE, India , June 23, 2016 /PRNewswire/ ... culture media market research report to its pharmaceuticals ... company profiles, product details and much more. ... market spread across 151 pages, profiling 15 companies ... now available at http://www.reportsnreports.com/reports/601420-global-cell-culture-media-industry-2016-market-research-report.html . ...
Breaking Biology Technology:
Cached News: