HOME >> BIOLOGY >> NEWS
Cold Spring Harbor Protocols features methods to understand embryos, clones, stem cells

COLD SPRING HARBOR, N.Y. (Fri., June 1, 2007) Cloning, X-chromosome inactivation, stem cells, and embryogenesis are hot areas of research at the moment, and protocols featured in this months release of Cold Spring Harbor Protocols (http://www.cshprotocols.org) will aid these studies. At the heart of these research areas are efforts to understand specific chemical modifications to proteins that surround DNA in its native cellular environment. These epigenetic marks are indicative of gene activity (off versus on), and can be used to understand processes underlying normal development, cellular reprogramming, and even events leading to cancer and other disease states.

One of the protocols, freely available at http://www.cshprotocols.org/cgi/content/full/2007/12/pdb.prot4767, describes how to characterize these epigenetic marks in native chromatin (i.e., the DNA is still bound to its associated proteins, as it would exist in its normal cellular environment). It specifies how to harvest native chromatin from a variety of cell types, fractionate the chromatin into manageable fragments, and then use an antibody to tag and isolate the proteins that possess specific epigenetic marks. Because the proteins remain bound to the DNA, the researchers can determine which genes (and other genomic elements) were off or on in the cell.

The June release of Cold Spring Harbor Protocols also features a set of methods to investigate embryogenesis in the frog, Xenopus laevis. Xenopus are commonly used for developmental studies because they produce large embryos that develop rapidly, are easy to maintain and manipulate, and can be induced to ovulate year-round.

One of the Xenopus-related protocols describes a method for surgically isolating and culturing a specific slice of tissue from Xenopus<
'"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
1-Jun-2007


Page: 1 2

Related biology news :

1. Cost-effective method for gene silencing is featured in Cold Spring Harbor Protocols
2. Springer launches Theoretical Ecology
3. Bioinformatics-related methods are featured in Cold Spring Harbor Protocols
4. Psychiatric Genomics Center established at Cold Spring Harbor Laboratory
5. Cold Spring Harbor Laoratory president wins Curtin Medal
6. Springer ecology book wins award for excellence
7. Cold Spring Harbor Protocols highlights reliable methods for gene and protein analyses
8. Springer author receives prestigious award in Beijing
9. A frenzy of fruit fly methods featured in Cold Spring Harbor Protocols
10. Cold Spring Harbor Laboratory scientists discover new gene that prevents multiple types of cancer
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:10/11/2018)... ... October 11, 2018 , ... The American College of Medical Informatics (ACMI) ... RN, PhD, Director of the National Library of Medicine (NLM), during the Opening Session ... is taking place Nov. 3 – 7. , In honor of Morris F. Collen, ...
(Date:10/5/2018)... , ... October 04, 2018 , ... EDETEK, the premier ... at the CDISC U.S. Interchange in Bethesda, MD on October 8-12, 2018. EDETEK is ... City, CA on November 5-6, 2018. , CDISC Interchanges are global events held ...
(Date:10/2/2018)... ... October 02, 2018 , ... Stem Cell Innovations (SCI), a global network ... stem cell therapy, has launched the pre-sale for their ICO with participants ... that will connect patients and stem cell specialists around the world. , ...
Breaking Biology News(10 mins):
(Date:10/2/2018)... Calif. (PRWEB) , ... October 02, 2018 , ... ... Undetected or ignored, contamination endangers the reliability and reproducibility of experimental data. Each ... contamination. , In this educational webinar, which is sponsored by Eppendorf , ...
(Date:9/28/2018)... (PRWEB) , ... September 28, 2018 , ... ... virtual events for tech innovators, engineers, and scientists from around the world, is ... the National University of San Marcos, as the winner of the LabRoots Summer ...
(Date:9/27/2018)... LOS ANGELES (PRWEB) , ... September 26, 2018 ... ... medical device and biologics manufacturer, debuted its focus® Pedicle Screw System today at ... Convention Center, September 26-29. , The Nvision focus Pedicle Screw System, ...
(Date:9/22/2018)... and WESTMINSTER, Colo. (PRWEB) , ... ... ... and Flagship Biosciences are excited to announce a strategic venture. The synergistic ... digital pathology platform provides complimentary and comprehensive biomarker profiling to empower clinical ...
Breaking Biology Technology:
Cached News: