HOME >> BIOLOGY >> NEWS
Cold Spring Harbor Protocols highlights reliable methods for gene and protein analyses

COLD SPRING HARBOR, N.Y. (Wed., Apr. 4, 2007) In their native form, the thousands of assorted proteins in our body are virtually indistinguishable. Scientists who want to examine the properties and functions of specific proteins, as well as the activities of individual genes, must rely on chemical tags to manipulate and visualize them. This month's release of Cold Spring Harbor Protocols highlights methods for creating and detecting specific proteins, as well as for characterizing the activities of specific genes during embryonic development. Two of the methods are freely available online (www.cshprotocols.org).

One of the freely available methods (http://www.cshprotocols.org/cgi/content/full/2007/8/pdb.prot4738) describes how to attach a small tag, called GST (glutathione-S-transferase), to a specific protein of interest. This is accomplished by growing bacteria that contain the DNA sequence for the GST tag adjacent to the gene for the protein of interest. When the bacteria grow, they express the "GST fusion protein," which consists of a GST tag attached to the desired protein. Using a chemical (glutathione) that binds specifically to GST, the GST fusion proteins can be easily sequestered and studied in more detail.

To test the activity of a certain gene during embryonic development, mice can be engineered to produce a "reporter" protein called -galactosidase, which functions as a proxy for the protein normally produced by the gene of interest. The second freely available method (http://www.cshprotocols.org/cgi/content/full/2007/8/pdb.prot4725) describes a strategy for staining transgenic mouse embryos engineered to express the -galactosidase protein. Once stained, the tissues with -galactosidase produce a brilliant blue pig
'"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
4-Apr-2007


Page: 1 2

Related biology news :

1. Cost-effective method for gene silencing is featured in Cold Spring Harbor Protocols
2. Springer launches Theoretical Ecology
3. Bioinformatics-related methods are featured in Cold Spring Harbor Protocols
4. Psychiatric Genomics Center established at Cold Spring Harbor Laboratory
5. Cold Spring Harbor Protocols features methods to understand embryos, clones, stem cells
6. Cold Spring Harbor Laoratory president wins Curtin Medal
7. Springer ecology book wins award for excellence
8. Springer author receives prestigious award in Beijing
9. A frenzy of fruit fly methods featured in Cold Spring Harbor Protocols
10. Cold Spring Harbor Laboratory scientists discover new gene that prevents multiple types of cancer
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:4/11/2017)... MELBOURNE, Florida , April 11, 2017 ... "Company"), a security technology company, announces the appointment of independent ... John Bendheim to its Board of Directors, furthering the ... ... behalf of NXT-ID, we look forward to their guidance and ...
(Date:4/5/2017)... Allen Institute for Cell Science today announces the launch ... dynamic digital window into the human cell. The website ... deep learning to create predictive models of cell organization, ... suite of powerful tools. The Allen Cell Explorer will ... resources created and shared by the Allen Institute for ...
(Date:4/5/2017)... April 4, 2017 KEY FINDINGS ... expand at a CAGR of 25.76% during the forecast ... the primary factor for the growth of the stem ... https://www.reportbuyer.com/product/4807905/ MARKET INSIGHTS The global stem cell ... application, and geography. The stem cell market of the ...
Breaking Biology News(10 mins):
(Date:10/12/2017)... ... October 12, 2017 , ... BioMedGPS announces expanded coverage of ... newest module, US Hemostats & Sealants. , SmartTRAK’s US Market for Hemostats and ... synthetic sealants and biologic sealants used in surgical applications. BioMedGPS estimates the market ...
(Date:10/11/2017)... ... October 11, 2017 , ... The ... context, enabling overexpression experiments and avoiding the use of exogenous expression plasmids. The ... transformative for performing systematic gain-of-function studies. , This complement to loss-of-function studies, ...
(Date:10/11/2017)... 11, 2017  VMS BioMarketing, a leading provider of patient ... Clinical Nurse Educator (CNE) network, which will launch this week. ... among health care professionals to enhance the patient care experience ... and other health care professionals to help women who have ... ...
(Date:10/11/2017)... ... October 11, 2017 , ... Singh ... orphan drug designation to SBT-100, its novel anti-STAT3 (Signal Transducer and Activator of ... SBT-100 is able to cross the cell membrane and bind intracellular STAT3 and ...
Breaking Biology Technology:
Cached News: