HOME >> BIOLOGY >> NEWS
Cold Spring Harbor Protocols highlights reliable methods for gene and protein analyses

COLD SPRING HARBOR, N.Y. (Wed., Apr. 4, 2007) In their native form, the thousands of assorted proteins in our body are virtually indistinguishable. Scientists who want to examine the properties and functions of specific proteins, as well as the activities of individual genes, must rely on chemical tags to manipulate and visualize them. This month's release of Cold Spring Harbor Protocols highlights methods for creating and detecting specific proteins, as well as for characterizing the activities of specific genes during embryonic development. Two of the methods are freely available online (www.cshprotocols.org).

One of the freely available methods (http://www.cshprotocols.org/cgi/content/full/2007/8/pdb.prot4738) describes how to attach a small tag, called GST (glutathione-S-transferase), to a specific protein of interest. This is accomplished by growing bacteria that contain the DNA sequence for the GST tag adjacent to the gene for the protein of interest. When the bacteria grow, they express the "GST fusion protein," which consists of a GST tag attached to the desired protein. Using a chemical (glutathione) that binds specifically to GST, the GST fusion proteins can be easily sequestered and studied in more detail.

To test the activity of a certain gene during embryonic development, mice can be engineered to produce a "reporter" protein called -galactosidase, which functions as a proxy for the protein normally produced by the gene of interest. The second freely available method (http://www.cshprotocols.org/cgi/content/full/2007/8/pdb.prot4725) describes a strategy for staining transgenic mouse embryos engineered to express the -galactosidase protein. Once stained, the tissues with -galactosidase produce a brilliant blue pig
'"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
4-Apr-2007


Page: 1 2

Related biology news :

1. Cost-effective method for gene silencing is featured in Cold Spring Harbor Protocols
2. Springer launches Theoretical Ecology
3. Bioinformatics-related methods are featured in Cold Spring Harbor Protocols
4. Psychiatric Genomics Center established at Cold Spring Harbor Laboratory
5. Cold Spring Harbor Protocols features methods to understand embryos, clones, stem cells
6. Cold Spring Harbor Laoratory president wins Curtin Medal
7. Springer ecology book wins award for excellence
8. Springer author receives prestigious award in Beijing
9. A frenzy of fruit fly methods featured in Cold Spring Harbor Protocols
10. Cold Spring Harbor Laboratory scientists discover new gene that prevents multiple types of cancer
11. Lighting up life: Cold Spring Harbor Protocols presents tips for creating glowing plants

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:9/15/2018)... ... September 14, 2018 , ... The first ever production picosecond-domain laser with a ... and purple tattoo inks - often the colors most resistant to laser removal. , ... of the American Society for Laser Medicine and Surgery, Inc. (ASLMS) investigated the safety ...
(Date:9/12/2018)... ... September 11, 2018 , ... Tennant ... introduce the new ChemXP™ line of high performance secondary containment coatings, designed to ... to provide Tennant Coatings’ elite certified installers with the needed solutions to protect ...
(Date:9/7/2018)... ... 05, 2018 , ... Following up on the launch of ... IPS-Integrated Project Services, LLC and G-CON Manufacturing have successfully designed and delivered the ... provides a pre-fabricated modular cleanroom infrastructure for the drug manufacturers’ requirements for both ...
Breaking Biology News(10 mins):
(Date:9/7/2018)... ... 06, 2018 , ... Cognition® Corporation , a software ... its upcoming user conference. , This year NAVIGATE2018 —the annual conference—will ... approaches to regulatory compliance and quality management. , “We’ve got a stellar lineup ...
(Date:9/1/2018)... ... August 31, 2018 , ... Rudina Thanasi, a scientifically validated ... biofield energy treated nutraceutical supplement used in combating inflammation and autoimmune disorders. ... as well as, organ-specific inflammation. The reported data is as follows: , ...
(Date:8/29/2018)... ... August 28, 2018 , ... ... announced today the successful completion of a $5.5 million funding round managed by ... Investment Fund, Commenda Capital, and Ellipsis Ventures. , “Our investment in Nanovis is ...
(Date:8/29/2018)... ... August 28, 2018 , ... CEO and founder of VetStem ... performed on an allogeneic (donor derived) stem cell product for osteoarthritis in canines ... in September. He is also the organizer of Breakout Session 4: Practitioner Primer ...
Breaking Biology Technology:
Cached News: