In experiments with rats, however, researchers led by led by Gerard J.M. Martens of the Nijmegen Center for Molecular Life Sciences (NCMLS) have demonstrated that such seemingly diverse combinations of symptoms can arise from a subtle imbalance in the activity of a single gene whose protein plays a key role in neural development.
The researchers studied the genetic differences between rats bred to be either resistant or susceptible to the drug apomorphine. A long history of studies has revealed that apomorphine-susceptible rats show many behavioral and biochemical differences from normal rats. What's more, the tendency to develop these differences depends on their exposure to stress in early life.
To search for genetic differences between the two types of rats, the researchers used microarrays, or so-called "gene chips," to analyze the activity of thousands of genes in the rats' brains. To their surprise, they discovered only a single genetic difference between the two groups of rats. The levels of activity of a gene called Aph-1b tended to be lower in the susceptible rats than in the non-susceptible rats. Importantly, this gene produces a protein that is a component of an enzyme called g-secretase, which plays a role in regulating many processes in the developing brain. The researchers found that, as a result of this lower level of Aph-1b protein, g-secretase showed reduced activity in the brains of the susceptible rats.
What's more, when the researchers tested various behaviors of "sublines" of rats with different levels of Aph-1b,
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Contact: Heidi Hardman
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Cell Press
16-Feb-2005