HOME >> BIOLOGY >> NEWS
DNA repair proteins monitored at double-strand break

Investigators at St. Jude Children's Research Hospital had a molecules eye view of the human cells DNA repair kit as it assembled on a double-strand break to link together the broken ends. Double-strand breaks are ruptures that cut completely across the twisted, ladder-like structure of DNA, breaking it into two pieces.

Using a technique developed specifically for this project, the St. Jude researchers could determine when repair proteins arrived at or around the DNA break and evaluate its repaireven when particular proteins shifted away from the break to make room for others. A report on this work appears in the May 7 online issue of "Nature Cell Biology."

The findings are important because disruption of the precise movement of these repair proteins can cause mutations, cell death or cancer, and the ability to track the process so closely will give researchers critical insights into what can go wrong with DNA repair. This could lead to novel ways to make cancer cells more sensitive to therapy by blocking their ability to repair double-stranded breaks caused by chemotherapy or radiation. It could also suggest new strategies for enhancing repair of double-stranded DNA caused by radiation, natural oxidants in food or the body and other toxins that can cause disease and aging.

"Prior to this work, there was no practical and efficient way to find and study the DNA repair proteins that organize themselves on and around a double-strand break in human cells," said Michael Kastan, M.D., Ph.D., St. Jude Cancer Center director. "Our approach solved that problem and allowed us to document the cells response to double-strand DNA breaks over time. The technique provides significantly more information about the proteins that repair DNA than is possible using the standard microscope-based approach previously used for such work." Kastan is the papers senior author.

A deficiency in two of these repair proteins, ATM and NBS1, leads to de
'"/>

Contact: Summer Freeman
summer.freeman@stjude.org
901-495-3061
St. Jude Children's Research Hospital
9-May-2007


Page: 1 2 3

Related biology news :

1. Where broken DNA is repaired
2. Fish eyes could hold clue to repairing damaged retinas in humans
3. Chromosome glue repairs damaged DNA
4. Faulty cell membrane repair causes heart disease
5. Scientists discover role of enzyme in DNA repair
6. Herpes virus hijacks DNA repair process
7. Analysis reveals extent of DNA repair army
8. Adult stem/progenitor cells repair of damaged brain, pancreas, kidney cells newly understood
9. Study links faulty DNA repair to Huntingtons disease onset
10. 2 heads are better than 1: 2 dysfunctional DNA repair pathways kill tumor cells
11. 3 proteins may play important role in nerve-cell repair

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/29/2018)... ... August 28, 2018 , ... Today, Bright Computing , ... In Science Technologies has implemented Bright Cluster Manager at the HudsonAlpha Institute for ... genomics and genetics research institute located in Huntsville, Ala. Its mission is four-fold: ...
(Date:8/26/2018)... YORBA LINDA, Calif. (PRWEB) , ... August 23, ... ... to the potential it has for increased sensitivity and specificity over more commonly ... of the instrumentation and the added burden of development and validation of methods ...
(Date:8/23/2018)... ... August 21, 2018 , ... CRISPR-Cas9 gene editing ... and species for the robust functional interrogation of complex biological processes. Gene editing ... based immunotherapies, the study of the autoimmunity, the understanding of host-pathogen dynamics that ...
Breaking Biology News(10 mins):
(Date:9/22/2018)... WESTMINSTER, Colo. (PRWEB) , ... ... ... Flagship Biosciences are excited to announce a strategic venture. The synergistic combination ... pathology platform provides complimentary and comprehensive biomarker profiling to empower clinical trials ...
(Date:9/22/2018)... (PRWEB) , ... September 20, 2018 , ... ... technologies and development solutions for drugs, biologics and consumer health products, today announced ... Relevant Today: Lipid-Based Drug Delivery in Modern Pharmaceutical Development,”. The one-day event is ...
(Date:9/22/2018)... LINDA, Calif. (PRWEB) , ... ... ... role of distinct cellular phenotypes in tissue function, development, and pathogenesis requires ... while preserving spatial information. , Multiplexed Ion Beam Imaging by Time-Of-Flight ...
(Date:9/15/2018)... ... , ... Next-generation genomic sequencing is transforming what is known about pediatric cancer ... past, the sheer size of genomics data has made sharing across the global scientific ... to advance cures by reducing the technological barriers to data sharing and analysis. Developed ...
Breaking Biology Technology:
Cached News: