HOME >> BIOLOGY >> NEWS
Darkness unveils vital metabolic fuel switch between sugar and fat

ract. Yet messenger RNA for CLPS (mClps) was found in the livers of mice exposed to prolonged dark, an unexpected finding.

They repeated the experiment in mice with natural, or "wild type," genomes and three strains of mutant mice with impaired circadian rhythms.

The wild-type mice and two of the mutant strains exposed to regular light-dark cycles showed no sign of the gene's expression in their livers it remained in the pancreas and stomach. The gene was expressed in the livers of some mice from a double-mutant strain that has absolutely no circadian response. All four genotypes of mouse kept in constant darkness had mClps expressed not only in their livers but in all peripheral tissue except the brain and kidneys.

"This is the first example of a gene that is turned on by darkness, where darkness itself is a signal," Lee said. "Twelve hours of darkness didn't do the job; it had to be at least 48 hours."

Tests showed it subsequently took five to seven hours of light exposure to inhibit the gene's expression in the liver. Taken together, these time delays pointed to the gene's expression being mediated by something in the blood. Another lab test showed elevated levels of 5'-AMP in the blood of mice exposed to constant darkness compared to those kept in the regular light-dark cycle.

To confirm the connection, the team injected 5'-AMP into mice exposed to a regular light-dark cycle. Three to four hours after injection, mClps was expressed in the livers of these mice and further tests showed expression in all tissues except the brain.

Injecting mice with 5'-AMP's more glamorous molecular cousins adenosine triphosphate (ATP), adenosine diphosphate (ADP), both vital to providing energy to cells, and the signaling molecule cyclic AMP -- did not produce the same effect.

The mice injected with 5'-AMP also were found to have a lower body temperature, a sign of torpor. Core body temperature measurements
'"/>

Contact: Scott Merville
scott.merville@uth.tmc.edu
713-500-3042
University of Texas Health Science Center at Houston
18-Jan-2006


Page: 1 2 3

Related biology news :

1. A new male-specific gene in algae unveils an origin of male and female
2. Womens Bioethics Project unveils The Scientist & the Ethicist podcast series
3. NIEHS Director unveils new strategic plan for environmental health sciences
4. University of Alberta researcher unveils worlds largest drug database
5. UVic unveils worlds most advanced seafloor observatory
6. Nano coalition unveils environmental, health and safety database
7. 3M Manchester research center to develop vital new science
8. Study led by Scripps Research scientist reveals little-known cell networks vital to circadian rhythm
9. Satellites play vital role in understanding the carbon cycle
10. ResearchChannel boosts awareness of vital research with video production program
11. Satellite data vital to UN climate findings

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/28/2020)... ... June 25, 2020 , ... In an upcoming episode scheduled for ... Point of Care Testing solutions (POCT). Check local listings for more info. , Today, ... offices and are shipped to labs throughout the country. Results are then available several ...
(Date:6/23/2020)... ... June 23, 2020 , ... ... healthcare, and Renovagen Ltd, a UK supplier and manufacturer of innovative portable renewable ... testing operations in the fight against the COVID-19 pandemic in Zambia. , One ...
(Date:6/23/2020)... ... June 23, 2020 , ... In its June 22 online post, business ... James L. Sherley, M.D., Ph.D., founder and director of stem cell biotechnology company ... June 16 that starting July 5 it would begin offering free tissue ...
Breaking Biology News(10 mins):
(Date:8/31/2020)... ... August 29, 2020 , ... ... manufacturing capability in Wuxi, China. The new plant operation will provide the local ... advanced technology. , “This facility will improve the flexibility of our offerings ...
(Date:8/15/2020)... ... 13, 2020 , ... Inc. magazine today revealed that Skysis ... of the nation’s fastest-growing private companies. The list represents a unique look at ... businesses. Intuit, Zappos, Under Armour, Microsoft, Patagonia, and many other well-known names gained ...
(Date:8/12/2020)... (PRWEB) , ... August 11, ... ... a biopharmaceutical cold chain validation engineering firm, is making available for free ... the need to incorporate concurrent transport simulation testing into today’s biologics licensing ...
(Date:8/7/2020)... ... August 06, 2020 , ... ... announces it has closed on the purchase of greenfield for a new, expanded ... and includes over 21 acres in the initial acquisition, with an option to ...
Breaking Biology Technology:
Cached News: