SAN FRANCISCO, April 27, 2007 Recent data from the Lung Allograft Rejection Gene expression Observational (LARGO) study provides compelling evidence that profiling gene expression in peripheral blood can detect organ rejection in lung transplant patients. Refining the Identification of Discriminatory Genes for Rejection in Lung Transplantation: The LARGO Study will be presented today by Shaf Keshavjee, M.D., Chair, Division of Thoracic Surgery, University of Toronto, and LARGO study lead, at the 27th Annual Meeting and Scientific Sessions of the International Society for Heart and Lung Transplantation (ISHLT).
Data from the study (Abstract 351) shows that the immune responses of lung transplant recipients can result in novel molecular signatures identifying the absence or presence of acute cellular rejection (ACR). That molecular signature can be identified using the quantitative real-time polymerase chain reaction (RT-PCR), a technology that measures the intracellular RNA levels of individual genes.
By distinguishing the patterns of gene expression in white blood cells (leukocytes), which fluctuate during the rejection of foreign tissue, PCR-based gene profiling can identify individual genes and pathways associated with the rejection status of transplanted lungs. In addition, the study shows that some of the genes whose expression changes during rejection of a transplanted lung are the same as those whose expression changes during the rejection of a transplanted heart.
Using microarray analysis, the researchers identified 259 genes that were differentially expressed for A0 and >A2 rejection grades (based on a standardized severity scale of 0-4). RT-PCR confirmed 14 of these genes as candidates for analysis, revealing a high correlation with those identified using microarray analysis. Seven of those genes were considered statistically significant.
"We are excited to confirm that new monitoring methods, such as non-invasi
Contact: Margaret Clark
International Society for Heart and Lung Transplantation