HOME >> BIOLOGY >> NEWS
Doing nature one better: Expanding the genetic code in living mammalian cells

La Jolla, CA Researchers at the Salk Institute for Biological Studies have developed a novel strategy to expand the natural repertoire of 20 amino acids in mammalian cells, including neurons, and successfully inserted tailor-made amino acids into proteins in these cells. In a powerful demonstration of the methods versatility, they then used unnatural amino acids to determine the operating mechanism of the molecular gates that regulate the movement of potassium ions in and out of nerve cells.

In the past, this type of engineering has been mainly restricted to bacteria or in yeast, and it was very challenging to efficiently incorporate unnatural amino acids in mammalian cells. But most biomedical questions have to be studied in the cells of higher organisms and animal models to arrive at meaningful answers, explains Lei Wang, Ph.D., an assistant professor in the Chemical Biology and Proteomics Laboratory, who led the current study published in the July issue of Nature Neuroscience.

The genetic code, which is shared by plants, animals and bacteria, includes 64 codons encoding 20 different amino acids and three stop signals. Being able to expand the code and insert non-natural amino not only greatly enhances researchers' ability and precision, but also provides novel tools for addressing challenging questions insurmountable with conventional means.

We had tried using conventional mutagenesis to introduce mutations into the potassium channel but it didnt give us any answers, says Paul A. Slesinger, Ph.D., an associate professor in the Peptide Biology Laboratory, who collaborated with Wang on the current study. Being able to incorporate bulky unnatural amino acids into living mammalian cells really made all the difference, he adds.

During his graduate studies, Wang pioneered a method to accommodate additional amino acids in bacteria. His approach mimicked the strategy every cell relies on to incorporate conventional amino acids
'"/>

Contact: Gina Kirchweger
kirchweger@salk.edu
858-453-4100, ext. 1340
Salk Institute
1-Jul-2007


Page: 1 2 3

Related biology news :

1. Doing it right: New book helps biologists conduct rigorous and reliable research
2. Ecologists work to link kids with nature
3. In nature, proteins sweep up nanoparticles
4. Pesticides choke pathway for nature to produce nitrogen for crops
5. Genomic signatures identify targeted therapies for lung cancer
6. Life-long learning -- nature provides natural inspiration for education
7. New synthetic self-assembling macromolecules mimic nature
8. Researchers untangle nature of regressive evolution in cavefish
9. DNA gives new perspectives to understand the mysteries of nature
10. Manipulating nature: Scientists query wildlife birth-control method
11. Beyond the DNA: Chemical signatures reveal genetic switches in the genome

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:3/22/2016)... India , March 22, 2016 /PRNewswire/ ... market research report "Electronic Sensors Market for Consumer ... Proximity, & Others), Application (Communication & IT, ... Geography - Global Forecast to 2022", published ... industry is expected to reach USD 26.76 ...
(Date:3/15/2016)... --> --> ... Research "Digital Door Lock Systems Market - Global Industry Analysis, ... global digital door lock systems market in terms of revenue ... forecast to grow at a CAGR of 31.8% during the ... enterprises (MSMEs) across the world and high industrial activity driving ...
(Date:3/11/2016)... --> --> ... Recognition Market by Technology (Pattern Recognition), by Component (Hardware, ... Type (On-Premises and Cloud), by Industry Vertical and by ... the global market is expected to grow from USD ... 2020, at a CAGR of 19.1%. , ...
Breaking Biology News(10 mins):
(Date:5/26/2016)... YORK , May 26, 2016 ... today that it will be a featured presenter at the ... in New York City at the ... Denis Corin , Q BioMed Inc. CEO, is scheduled to ... will cover the company,s business strategy, recent developments and outline ...
(Date:5/25/2016)... ... , ... Lajollacooks4u has become a rising hotspot for specialized team building events ... attractions. Fortune 500 companies, such as Illumina, Hewlett-Packard, Qualcomm and Elsevier, have traveled ... , Each event kicks off with an olive oil and salt-tasting competition. From ...
(Date:5/25/2016)... ... ... at the University of Athens say they have evidence that the variety of different ... lead to one good one. Surviving Mesothelioma has just posted an article on the ... 98 mesothelioma patients who got a second kind of drug therapy after ...
(Date:5/24/2016)... May 24, 2016   MedyMatch Technology Ltd ., the ... intelligence, real-time decision support tools in the emergency room, announced ... 2016 Israeli Advanced Technology Industries (IATI) BioMed Conference. ... 15th National Life Sciences and Technology Week, and ... Intercontinental Hotel in Tel Aviv, Israel . ...
Breaking Biology Technology:
Cached News: