HOME >> BIOLOGY >> NEWS
Embryonic stem cells do better on bumpy nanoscale mattress

Nothing in the cellular world is flat. Even the flattest of basement membranes has topography; bumps, if you like, beneath the cellular mattress.

Unlike the princess kept awake by the pea, human embryonic stem (HES) cells do better when cultured on a substrate deliberately printed with nanoscale grooves and ridges, according to researchers from the University of WisconsinMadison.

The researchers used soft lithography to stamp polyurethane substrates with a nano- micron scale topography; a rugged cellular landscape ranging from a few billionths to a few millionths of a meter in altitude.

The HES cells in culture seemed to appreciate the bumps. A line of HES cells grown for five days on the artificial ridges and grooves kept their stemness, their self-renewing phenotype, far better than HES cells plated on standard flat culture surfaces, according to Daniel McFarlin, K.J. Finn, and Chris Murphy of the University of Wisconsins School of Veterinary Medicine, who teamed with P.F. Nealey of the Universitys Department of Chemical Engineering.

Unlike stem cells derived from adult tissues, which have a limited number of cell doublings, embryonic stem cells cultured under the right conditions have the potential to divide indefinitely, without losing their pluripotent properties. But until now, HES cell cultures had a tendency to spontaneously differentiate, that is, to veer off without warning into a developmental pathway. HES cell cultures have to be closely watched to remove any of these spontaneously differentiated colonies.

Researchers have looked at surface chemistry, growth factors, and mechanical forces as factors in runaway stem cell differentiation, but topography is a new dimension for HES, say Murphy and McFarlin, and a highly promising one.

This is the first demonstration that the physical topography, using controlled feature dimensions, of cell culture surfa
'"/>

Contact: John Fleischman
jfleischman@ascb.org
513-929-4635
American Society for Cell Biology
13-Dec-2006


Page: 1 2

Related biology news :

1. Embryonic selection of sex avoids conceiving blind children
2. Embryonic patterning makes the feathers fly
3. AAAS urges US President to sign Embryonic Stem Cell Act
4. Embryonic stem cells accrue genetic changes
5. NIH selects Burnham Institute for Exploratory Center for Human Embryonic Stem Cell Research
6. Embryonic law and order
7. Embryonic stem cells treated with growth factor reverse hemophilia in mice
8. Embryonic stem cells correct congenital heart defect in mouse embryos
9. Newly created cancer stem cells could aid breast cancer research
10. ESF EURYI award winner aims to stop cancer cells reading their own DNA
11. OHSU turns mouse into factory for human liver cells

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/31/2019)... ... 29, 2019 , ... FileWave, the multi-platform endpoint management solution ... of version 13.1. The new version extends FileWave’s mobile device management features on ... release functionality. Version 13.1 builds on FileWave’s version 13 that was released in ...
(Date:5/31/2019)... ... ... Enso Discoveries Chief Executive Officer, Patrick Farley, is honored to give back to ... his gratitude and respect for those who have served their country. In honor of ... presented a check for $1,000 to the Green Beret Foundation, via former US Army ...
(Date:5/24/2019)... WOODLANDS, Texas (PRWEB) , ... May 24, 2019 , ... ... been published and is now available on the company’s global website. , Crystallography Times ... on single crystal X-ray diffraction. It serves the X-ray analysis community by presenting the ...
Breaking Biology News(10 mins):
(Date:5/2/2019)... , ... May 02, 2019 , ... Stay on top ... medical device and food industries. Access to all webinars is free, so be sure ... in your field! , Visit http://www.xtalks.com to see our upcoming webinars: , ...
(Date:4/25/2019)... ... , ... Frank is an albino, deaf Great Dane. His owners rescued him when he was ... issues. Despite his hearing impairment, he was always an active and playful pup. Frank bonded ... every day. As the two grew, playtime became rougher and Frank ended up injuring his ...
(Date:4/16/2019)... ... April 16, 2019 , ... ... their project partner, the U.S. Army Center for Environmental Health Research (USACEHR), with ... Combined In-Licensing/Out-Licensing.” , Blue Sources, a veteran-owned company located at the Frederick Innovative ...
(Date:4/12/2019)... , ... April 12, 2019 , ... ... developments in neurotechnology and will educate about innovative tools and devices being used ... local listings for more information. , This segment of Advancements will focus on ...
Breaking Biology Technology:
Cached News: