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Encoded metallic nanowires reveal bioweapons

This press release is also available in German.

When dangerous infectious diseases or biological weapons are suspected, fast help is required. The first step is a reliable, sensitive, and unambiguous, yet also fast and simple, identification of the pathogen; preferably, this test should be carried out on the spot, not in a laboratory. Portable miniature biodetection systems that can detect multiple pathogens simultaneously would be ideal for this task. American researchers from Lawrence Livermore National Laboratory led by Jeffrey Tok, in collaboration with groups at Stanford University, University of California at Davis, and Oxonica Inc (formerly Nanoplex Technologies Inc). have now developed a new basis for such a multiplex device: they are using silver and gold "striped" nanowires as supports for simultaneous immunological tests for various pathogens. Individual patterns of stripes act in the role of "barcodes". These "nanobarcoded" particles are manufactured by Oxonica Inc using template-assisted electrochemical deposition of metals within the tiny cylindrical pores of alumina membranes. When deposited gold and silver are alternated in a defined way, nanowires with different characteristic stripe patterns are produced. The pattern of optical reflections from each sequence of stripes can later be unambiguously recognized--just like a barcode.

Antibodies aimed at specific pathogens can be attached to these wires. For their test runs, Tok and his colleagues selected harmless model substances to stand in for anthrax spores, smallpox virus, and protein toxins such as ricin and botulinum toxin. If a simultaneous test for all of these is desired, the anthrax antibody would be attached to stripe pattern 1, the smallpox antibodies to stripe pattern 2, and the toxin antibodies to stripe pattern 3, for example. If the corresponding model pathogen is prese
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Contact: Jeffrey B.-H. Tok
tok2@llnl.gov
925-423-1549
John Wiley & Sons, Inc.
10-Aug-2006


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