HOME >> BIOLOGY >> NEWS
Gene silencing directs muscle-derived stem cells to become bone-forming cells

BALTIMORE - Using a relatively new technology called RNA interference to turn off genes that regulate cell differentiation, University of Pittsburgh researchers have demonstrated they can increase the propensity of muscle-derived stem cells (MDSCs) to become bone-forming cells. Based on these results, the investigators believe that by turning off specific genetic factors they can control the capacity of MDSCs as a means of treating various musculoskeletal diseases and injuries.

RNA interference is a simple yet powerful technique that uses short interfering RNAs (siRNAs) small molecules that prevent a gene from being expressed to turn off the production of specific proteins in a cell. In their study, the Pitt researchers generated siRNAs to two mouse genes: MyoD1, a master gene that regulates the formation of muscle cells or fibers (myogenesis), and Smad6, which encodes a molecule that specifically inhibits a cell's ability to respond to bone-forming, or osteogenic, signals.

When the researchers examined cultured cells in which myogenesis was inhibited, they found a significant increase in the cells' bone-forming potential. However, contrary to their expectations, the researchers did not observe any bone formation when the cells were implanted in skeletal muscle. Yet, when they turned off ostegenic inhibition in these same cells using Smad6 siRNA prior to implanting them in mice, 60 percent of the mice developed radiologically detectable bone within three weeks.

"By understanding the genetic mechanisms that regulate a cell's propensity to differentiate into one type of cell line over another, we may be able to regulate their ability to generate bone for the treatment of various diseases and injuries of the musculoskeletal system, such as osteoporosis or severe fractures," said first author Jonathan B. Pollett, Ph.D., research associate, department of orthopaedic surgery, Children's Hospital of Pittsburgh. Corresponding author Johnny Huard,
'"/>


1-Jun-2006


Page: 1 2

Related biology news :

1. Cost-effective method for gene silencing is featured in Cold Spring Harbor Protocols
2. Newly identified mechanism for silencing genes points to possible anti-cancer strategies
3. Pathway toward gene silencing described in plants
4. miRNA-mediated silencing of mRNAs
5. New mechanism for essential genome-wide gene silencing identified
6. Proteins role in hemoglobin gene silencing identified
7. Prediction of a prokaryotic RNA-silencing system
8. Twin molecular scissors link creation of microRNAs with gene-silencing
9. Gene silencing technique offers new strategy for treating, curing disease
10. Molecular steps involved in the creation of gene-silencing microRNAs identified
11. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma

Post Your Comments:
(Date:8/18/2015)... Aug. 17, 2015 Research and Markets ... the "Global Biometric Authentication & Identification Market: ... & Forecast, 2015-2020" report to their offering. ... identification systems market is expected to grow at ... 2020 and generate over $25 billion (approximately) by ...
(Date:8/12/2015)... 12, 2015  Synaptics Inc. (NASDAQ: SYNA ... today announced that four of its fingerprint ID ... officially been named FIDO Certified™ by the Fast ... certification, Synaptics, Natural ID™ fingerprint solutions are fully ... (UAF) standard and are interoperable among other products ...
(Date:8/6/2015)... and TELTOW, Germany , ... 2015 Siggraph Conference, SensoMotoric Instruments (SMI) shows ... reality , based on Epson,s Moverio BT-200 ... tracking platform. With this new solution, unprecedented quality and ... hands-free interaction with context-sensitive displays. For the first time, ...
Breaking Biology News(10 mins):Global Biometric Authentication & Identification Market 2015: Focus On Modality, Motility & Application - Estimation & Forecasts to 2020 for the $25 Billion Industry 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 2Synaptics Announces FIDO Certification of Natural ID Fingerprint Solutions 3SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 2SMI Shows First Eye Tracking Upgrade for Augmented Reality Glasses 3
(Date:9/2/2015)... 2015 Research and ... of Jain PharmaBiotech,s new report "Biochips and ... their offering. This report is an ... applications. The report starts with a description of ... Technologies include array comparative genomic hybridization (CGH), copy ...
(Date:9/2/2015)... Virginia , September 2, 2015 ... of the winners of the 2015 BeHEARD science challenge, ... that offers rare disease researchers, access to the latest ... use its drug repositioning and pathway analytics capability to ... Wylder Nation Foundation in the study of Niemann-Pick Disease ...
(Date:9/2/2015)... SAN FRANCISCO, Calif. , Sept. 2, 2015 /PRNewswire/ ... that Bonnie H. Anderson , president and chief ... Healthcare Conference on Wednesday, September 16, 2015 at 10:30 ... . The live audio webcast and ... http://investor.veracyte.com . The webcast will be available shortly after ...
(Date:9/2/2015)... ... September 02, 2015 , ... Whitehouse ... will be attending Pharma Ed Resources upcoming educational seminar and training session on ... speakers, this timely conference will provide Updates & Case Studies on the Latest ...
Breaking Biology Technology:Global Biochips and Microarrays Technologies, Markets and Companies Report 2015 with Market Size in 2014 and Projected Value for the Years 2019 and 2024 2Biovista Sponsors Wylder Nation Foundation's Research on Acid Sphingomyelinase Deficiency 2Veracyte to Present at Morgan Stanley Global Healthcare Conference 2Whitehouse Laboratories to Participate in Pharm Ed Resources Extractables, Leachables, & Elemental Impurities Educational Seminar 2Whitehouse Laboratories to Participate in Pharm Ed Resources Extractables, Leachables, & Elemental Impurities Educational Seminar 3
Cached News: