HOME >> BIOLOGY >> NEWS
Gene silencing directs muscle-derived stem cells to become bone-forming cells

BALTIMORE - Using a relatively new technology called RNA interference to turn off genes that regulate cell differentiation, University of Pittsburgh researchers have demonstrated they can increase the propensity of muscle-derived stem cells (MDSCs) to become bone-forming cells. Based on these results, the investigators believe that by turning off specific genetic factors they can control the capacity of MDSCs as a means of treating various musculoskeletal diseases and injuries.

RNA interference is a simple yet powerful technique that uses short interfering RNAs (siRNAs) small molecules that prevent a gene from being expressed to turn off the production of specific proteins in a cell. In their study, the Pitt researchers generated siRNAs to two mouse genes: MyoD1, a master gene that regulates the formation of muscle cells or fibers (myogenesis), and Smad6, which encodes a molecule that specifically inhibits a cell's ability to respond to bone-forming, or osteogenic, signals.

When the researchers examined cultured cells in which myogenesis was inhibited, they found a significant increase in the cells' bone-forming potential. However, contrary to their expectations, the researchers did not observe any bone formation when the cells were implanted in skeletal muscle. Yet, when they turned off ostegenic inhibition in these same cells using Smad6 siRNA prior to implanting them in mice, 60 percent of the mice developed radiologically detectable bone within three weeks.

"By understanding the genetic mechanisms that regulate a cell's propensity to differentiate into one type of cell line over another, we may be able to regulate their ability to generate bone for the treatment of various diseases and injuries of the musculoskeletal system, such as osteoporosis or severe fractures," said first author Jonathan B. Pollett, Ph.D., research associate, department of orthopaedic surgery, Children's Hospital of Pittsburgh. Corresponding author Johnny Huard,
'"/>


1-Jun-2006


Page: 1 2

Related biology news :

1. Cost-effective method for gene silencing is featured in Cold Spring Harbor Protocols
2. Newly identified mechanism for silencing genes points to possible anti-cancer strategies
3. Pathway toward gene silencing described in plants
4. miRNA-mediated silencing of mRNAs
5. New mechanism for essential genome-wide gene silencing identified
6. Proteins role in hemoglobin gene silencing identified
7. Prediction of a prokaryotic RNA-silencing system
8. Twin molecular scissors link creation of microRNAs with gene-silencing
9. Gene silencing technique offers new strategy for treating, curing disease
10. Molecular steps involved in the creation of gene-silencing microRNAs identified
11. Novel gene-silencing nanoparticles shown to inhibit Ewings sarcoma

Post Your Comments:
(Date:10/31/2014)... live in Nuristan Province – some 60 years after ... glands are more valuable than gold , Study appears ... , NEW YORK (October 31, 2014) – More ... strange deer with vampire-like fangs still persists in the ... research team led by the Wildlife Conservation Society (WCS), ...
(Date:10/31/2014)... wide part of the electromagnetic spectrum from the infrared ... Sweden. The MAX IV facility presents a range of ... parts in a storage - ring synchrotron system that ... metres. Nevertheless, if these various challenges can be addressed ... source brightness and transverse coherence will be possible. ...
(Date:10/31/2014)... air out of a nagging mystery about the development of ... to why animal species didn,t flourish sooner, once sufficient oxygen ... end of the Proterozoic period, about 800 million years ago ... most researchers think there also was plenty of oxygen? , ... all. , In a study published Oct. 30 in ...
Breaking Biology News(10 mins):Strange, fanged deer persists in Afghanistan 2A new generation of storage -- ring 2Lack of oxygen delayed the rise of animals on Earth 2
(Date:10/31/2014)... 2014 Having access to safe drinking ... battle in many corners of the globe. It’s a ... in northwestern Bolivia where many have been sickened by ... Fairfield University’s School of Engineering has been working to ... (UAC), “the united college for the peasants.” A branch ...
(Date:10/31/2014)... 2014 Caustic soda is utilized in the ... At present, China takes lead in the global caustic soda ... a number of facilities in the USA, Germany, Brazil and ... soda market in terms of output and production capacities. , ... average by 3% per year in the years ahead. The ...
(Date:10/31/2014)... IL (PRWEB) October 30, 2014 ... Insight Product Development’s decades-long heritage in successful medical ... world context for what it takes to successfully ... space. As part of a two-hour on-site class, ... Kellogg school of business, McCormick School of Engineering, ...
(Date:10/31/2014)... MA (PRWEB) October 30, 2014 ... Latin America were hosted by Charm Sciences at ... During their week in the US, the participants ... residue control, hygiene management, and process verification, and ... facility. , This was the first visit to ...
Breaking Biology Technology:Fairfield University School of Engineering project reduces illness in rural community 2Fairfield University School of Engineering project reduces illness in rural community 3China to Dominate Caustic Soda Market Through 2018, Says Merchant Research & Consulting in Its New Study 2Insight Product Development Gives Northwestern University Students Foundation for Innovation Success 2Insight Product Development Gives Northwestern University Students Foundation for Innovation Success 3Charm Sciences Hosts Executives from Latin America for Dairy Safety Executive Seminar 2
Cached News: