HOME >> BIOLOGY >> NEWS
Hepatitis C helicase unwinds DNA in a spring-loaded, 3-step process

The process by which genes are duplicated is mysterious and complex, involving a cast of characters with diverse talents and the ability to play well with others in extremely close quarters. A key player on this stage is an enzyme called a helicase. Its job is to unwind the tightly coiled chain of nucleic acids the DNA or RNA molecule that spells out the organisms genetic code so that another enzyme, a polymerase, can faithfully copy each nucleotide in the code.

Researchers at the University of Illinois, Yale University and the Howard Hughes Medical Institute have shed new light on how the Hepatitis C helicase plays this role, using a technique developed at Illinois that can track how a single molecule of RNA or DNA unwinds. Their research findings appear tomorrow in the journal, Science.

Getting at the underlying mechanisms of replication is no easy task. Structural studies involve crystallizing the DNA-protein complexes to see how they interact. Biochemists look at the agents of a reaction, the energy used and how much time lapses between steps. Such studies measure the behavior of hundreds of thousands of molecules at a time, and the results describe a whole population of reactions.

Using single-molecule fluorescence analysis, the research team tracked how the hepatitis C helicase, NS3, unwound a duplexed DNA molecule tagged with a fluorescent label on each strand of its double-stranded region. (The NS3 helicase is primarily involved in unwinding the single-stranded RNA of the hepatitis virus, but it can also act on DNA. This suggests that the helicase plays a role in unwinding double-stranded host DNA during infection. The duplex created for the experiment included both single- and double-stranded DNA; fluorescent labels were located in the double-stranded region.)

By tracking the gradually increasing distance between the two marked nucleotides as the strands separated in an unwinding event, the researchers were able
'"/>

Contact: Diana Yates
diya@uiuc.edu
217-333-5802
University of Illinois at Urbana-Champaign
26-Jul-2007


Page: 1 2 3

Related biology news :

1. Hepatitis B drug can compromise HIV treatment
2. Hepatitis E takes a piggyback
3. New hope for Hepatitis C research
4. Hepatitis C therapy: Inhibiting newly discovered microDNA molecule
5. New insights into protein synthesis and Hepatitis C infections
6. Hepatitis B accounts for 40 percent of missing Asian women
7. Hepatitis C complicated by morphine withdrawal
8. Identification of a protein important for Hepatitis B replication
9. Identification of specific genes predicts which patients will respond to Hepatitis C treatment
10. Hepatitis C virus linked to non-Hodgkins lymphoma
11. N.C. A&T food scientist develops process for allergen-free peanuts

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/1/2020)... ... April 30, 2020 , ... Flagship Biosciences , ... Inc., a biopharmaceutical and regulatory services advisor. The companies now deliver regulatory strategy ... Corritori are taking a collaborative approach to empower more confident and informed regulatory ...
(Date:4/30/2020)... ... ... Pebble Labs USA Inc. , a biotechnology company developing solutions that ... of improving global food security, announced today that they have been selected as one ... Ag-Tech ' virtual event, a global initiative to drive innovation in feeding future generations. ...
(Date:4/28/2020)... ... April 27, 2020 , ... Ofee.com, an online ... three teenagers with an idea to help millions of people during the coronavirus ... great need and suffering, an outlet has been created for people to meet ...
Breaking Biology News(10 mins):
(Date:5/1/2020)... NEW YORK (PRWEB) , ... April 30, 2020 ... ... Ideas Awards were announced today, honoring the businesses, policies, projects, and concepts that ... is a revolutionary bioprinter for living cells, which has taken out the top ...
(Date:4/23/2020)... ... 22, 2020 , ... Across the country, there is an ... Virginia has only been receiving 50% to 7.7% of the personal protective equipment ... Northam told the Virginia Mercury, “While we appreciate what we’ve received from the ...
(Date:4/22/2020)... ... April 21, 2020 , ... Aniara Diagnostica is pleased ... , Aniara Diagnostica, a multi-national distributor of diagnostic and research agents, as well ... product quality and cost-efficient delivery. , For Research Use Only. Not ...
(Date:4/18/2020)... ... April 17, 2020 , ... ... Small Business Innovation Research (SBIR) program to support the expansion of its digital ... the NSF, which conducts a rigorous merit-based review process, adds to NeuroFlow’s ...
Breaking Biology Technology:
Cached News: