HOME >> BIOLOGY >> NEWS
Imaging techniques permit scientists to follow a day -- or 4 -- in the life of a cell

COLD SPRING HARBOR, N.Y. -- The movement and growth of cells are critical for normal physiological processes, and--when perturbed--can result in negative outcomes such as tumor formation. Understanding how live cells function is therefore invaluable for molecular and cellular biologists, and advanced techniques to visualize cells in action are of great importance. The current issue of Cold Spring Harbor Protocols (www.cshprotocols.org) addresses these concerns with two freely accessible protocols: one for inserting 'reporter' proteins into cells to monitor what's going on inside, and another for maintaining the cells under a microscope for long-term observation.

The first protocol, available at http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4657, details a procedure to inject proteins into individual cells. The injected protein can be fluorescently labeled, so scientists can track the glowing protein with a microscope and observe what it's doing and where it's going inside the cell. This microinjection technique is similar to those that are used to create cloned and transgenic organisms, and it can also be applied to more complex assays--including laser photobleaching and fluorescent speckle microscopy--that yield specific insights into cellular processes.

A second freely available protocol (http://www.cshprotocols.org/cgi/content/full/2007/1/pdb.prot4660) outlines the construction and use of an enclosed microscope chamber. This device is designed to optimize the culture environment for cells--allowing them to grow as they would normally--while optimizing the conditions for viewing them by microscopy. This enables researchers to monitor the cells under a microscope for long time periods (in excess of four days), during w
'"/>

Contact: Maria Smit
smit@cshl.edu
516-422-4127
Cold Spring Harbor Laboratory
3-Jan-2007


Page: 1 2

Related biology news :

1. FSU leads botanical research into 21st century: Deep South Plant Specimen Imaging Project
2. ESA POLinSAR 2007 -- Imaging forests in 3-D
3. Imaging pinpoints brain regions that see the future
4. Brookhaven Lab and Mount Sinai form joint Center for Translational Biomedical Imaging
5. Imaging single molecules opens new way to study gene expression, protein production
6. SNM awards $25,000 Mallinckrodt Seed Grant in Molecular Imaging/Nuclear Medicine Research
7. Imaging study links key genetic risk for Alzheimers disease to myelin breakdown
8. Society of Nuclear Medicine announces collaborative working agreement with Bio-Imaging Technologies
9. Imaging goes 3-D: exquisite PET/CT image captures SNMs 2005 Image of the Year
10. New techniques redefine assessment of liver disease
11. Cloning techniques produce FDA-approved antibiotic

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/31/2018)... and CARDIFF, Wales (PRWEB) , ... August 30, ... ... Agency. Martha Sanchez, senior scientist, IBM Research. Jacklyn Novak, infrared materials engineer, LightPath ... Katherine Johnson, the NASA mathematician who just celebrated her 100th birthday. Today, they ...
(Date:8/29/2018)... , ... August 29, 2018 ... ... validates first non-invasive technology to identify specific chromosomal abnormalities using morphological assessment ... Ovation Fertility and Australia-based Life Whisperer suggests that artificial ...
(Date:8/23/2018)... (PRWEB) , ... August 21, 2018 , ... ... the genomes of diverse cell types and species for the robust functional interrogation ... tool for the development of T-cell based immunotherapies, the study of the autoimmunity, ...
Breaking Biology News(10 mins):
(Date:8/23/2018)... ... August 22, 2018 , ... Crucial Data Solutions, Inc. ... it the first fully-featured app for electronic data capture (EDC) and electronic participant ... constitute 99 percent of the market share for mobile devices worldwide; in turn, ...
(Date:8/21/2018)... ... August 21, 2018 , ... The Connecticut Technology Council (CTC) ... of the fastest growing technology companies in Connecticut. This year’s group of top-ranked companies ... October 3, at the Connecticut Convention Center in Hartford. A complete list of the ...
(Date:8/17/2018)... ... August 16, 2018 , ... Ambry Genetics Corporation (“Ambry”), a ... were published today in JAMA Oncology, identifying new and confirming known hereditary breast ... Every year hereditary breast cancer affects more than 20,000 women while hereditary ovarian ...
(Date:8/14/2018)... ... August 14, 2018 , ... Buprenorphine (BUP) is ... BUP is often prescribed as a sublingual co-formulation with the opioid antagonist naloxone ... participants will learn from a review of the pharmacokinetics of BUP, NBP, and ...
Breaking Biology Technology:
Cached News: