HOME >> BIOLOGY >> NEWS
In a first, Einstein scientists discover the dynamics of transcription in living mammalian cells

August 6, 2007 (BRONX, NY) Transcription the transfer of DNAs genetic information through the synthesis of complementary molecules of messenger RNA forms the basis of all cellular activities. Yet little is known about the dynamics of the process how efficient it is or how long it takes. Now, researchers at the Albert Einstein College of Medicine of Yeshiva University have measured the stages of transcription in real time. Their unexpected and surprising findings have fundamentally changed the way transcription is understood.

The researchers used pioneering microscopy techniques developed by Dr. Robert Singer, co-chair of anatomy & structural biology at Einstein and senior author of the study, which appears in the August issue of Nature Structural & Molecular Biology.

The study focused on RNA polymerase II--the enzyme responsible for transcription. During transcription, growing numbers of RNA polymerase II molecules assemble on DNA and then synthesize RNA by sequentially recruiting complementary RNA nucleotides.

To visualize the transcription process, the researchers used living mammalian cells, each of which contained 200 copies of an artificial gene that they had inserted into one of the cells chromosomes. Then, by attaching fluorescent tags to RNA polymerase II, they were able to closely monitor all three phases of the transcription process: binding of the enzyme molecules to DNA, initiation (when the enzyme links the first few RNA nucleotides together) and elongation (construction of the rest of the RNA molecule). As they observed the RNA polymerase II molecules attaching to DNA and making new RNA, they saw many cases where enzyme molecules attached and then promptly fell off.

One surprising finding was how inefficient the transcription process really is, particularly during its first two stages, says Dr. Singer. It turns out that only one percent of polymerases that bind to the gene actually remain on to help in
'"/>

Contact: Karen Gardner
kgardner@aecom.yu.edu
718-430-3101
Albert Einstein College of Medicine
6-Aug-2007


Page: 1 2

Related biology news :

1. Which came first, the moth or the cactus?
2. In a first, scientists develop tiny implantable biocomputers
3. Einstein researchers prototype vaccine could provide improved protection against tuberculosis
4. Einstein researchers discover radiation-eating fungi
5. Einsteins Dr. Vern Schramm elected to the National Academy of Sciences
6. Einstein researchers discover how a key dietary vitamin is absorbed
7. NCI funds Einstein and U. Albany NanoCollege to make worlds smallest cancer detection device
8. Team at CNSE and Einstein receive grant to develop worlds smallest cancer detection device
9. Einstein researchers demonstrate a novel approach to treating AIDS
10. Einsteins Dr. E. Richard Stanley receives 2006 E. Donnall Thomas Prize
11. Einstein researchers find key to unlocking worlds deadliest malaria parasite

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:8/26/2020)... ... ... Modality Solutions, a biopharmaceutical cold chain validation engineering firm, is pleased ... the most prestigious ranking of the nation’s fastest-growing private companies. Modality Solutions made the ... revenue growth of 71 percent. , The Inc. 5000 list represents a unique ...
(Date:8/21/2020)... Calif. (PRWEB) , ... August 19, 2020 , ... ... both Salimetrics LLC and SalivaBio LLC, businesses dedicated to Salivary Bioscience for more ... of the recently published book by Springer, " Salivary Bioscience: Foundations of Interdisciplinary ...
(Date:8/12/2020)... ... August 11, 2020 , ... Both ... NJ, have entered into license agreements with Housey Pharma’s HMI subsidiary to gain ... and J&J have annual Research and Development spending in excess of US $10 ...
Breaking Biology News(10 mins):
(Date:8/15/2020)... SCOTTSDALE, Ariz. (PRWEB) , ... August 13, 2020 ... ... 4254 on its annual Inc. 5000 list, the most prestigious ranking of the ... successful companies within the American economy’s most dynamic segment—its independent small businesses. Intuit, ...
(Date:8/7/2020)... ... August 06, 2020 , ... VGXI, ... it has closed on the purchase of greenfield for a new, expanded manufacturing ... includes over 21 acres in the initial acquisition, with an option to purchase ...
(Date:7/31/2020)... ... 29, 2020 , ... G-CON Manufacturing, the leader in off-site ... FOR LIFE.” The adoption of this taglines comes at a time of tremendous ... to provide patients with urgently needed vaccines and other lifesaving pharmaceutical treatments. G-CON’s ...
(Date:7/31/2020)... ... July 29, 2020 , ... eSource has long been touted ... will cover the history of eSource, the reasons it did not take off as ... to site source, the industry is moving towards capturing data electronically for clinical trials ...
Breaking Biology Technology:
Cached News: