Differentiation is the process by which embryonic stem cells gradually turn into function-specific types of adult cells or so-called "cell lineages," including skin, heart or brain cells.
The main challenge facing stem cell research is that of guiding differentiation along these well-defined, controlled lineages. Stem cells grown in the laboratory tend to differentiate in an uncontrolled manner, resulting in a mixture of cells of little medical use.
Now, University of Wisconsin-Madison researchers at the NSF-funded Materials Research Science and Engineering Center (MRSEC) have shown that by straining mechanically the cells as they grow, it is possible to reduce significantly and almost eliminate the uncontrolled differentiation of stem cells.
In an article in the March issue of Advanced Functional Materials, the team reports on a liquid crystal-based cell culture system that promises new ways of achieving real-time control over interactions between synthetic materials and human embryonic stem cells, including the possibility of straining embryonic stem cells as they grow.
"Stem cells tend to be smaller and have a slightly more compact shape than the differentiated cells," says chemical and biological engineer Sean Palecek. "Differentiated cells appear to be much more spread and they appear to exert different levels of force on the matrix in which they are grown. That force can be read to a liquid crystal. Through simple changes of liquid crystal texture and color, our cell culture system is able to report, in real time, the cell interactions with the underlying support on which they are grown."
Currently, researchers have several methods of monitoring cell differentiation. The easiest, says Palecek, is t
Contact: Sean Palecek
University of Wisconsin-Madison