LIVERMORE, Calif. -- It may sound counterintuitive to use a microbial protein to improve water quality.

But some bacteria are doing just that to protect themselves from potentially toxic nanoparticles in their own environments, and clean up crews of the future could potentially do the same thing on a larger scale.

A team from Lawrence Livermore National Laboratory, UC Berkeley and Lawrence Berkeley National Laboratory found that bacteria from an abandoned mine excrete proteins that cause metal nanoparticles to aggregate. The bacteria are binding and immobilizing the metals in the nanoparticles and the nanoparticles themselves, which are potentially toxic to the bacteria.

Sulfate-reducing bacteria can cause heavy metals such as zinc (Zn) to precipitate and form nanoparticles. However, these particles are able to move freely because they are so small (typically 2-6 nanometers in diameter) and can redissolve if conditions change.

In the case of the mine bacteria, the researchers showed that the bacteria are causing the nanoparticle aggregation, thereby protecting themselves. When the metal nanoparticles aggregate, they dont move as easily and are less soluble.

Using secondary ion mass spectrometry, transmission electron microscopy and infra-red spectroscopy, the scientists were able to study whether protein contributes to the formation of densely aggregated nanoparticulate zinc sulfide spheroids.

They also studied whether various amino acids induce rapid aggregation in metal-sulfide nanoparticles.

The answer was yes in both cases.

This demonstrates an extracellular biomineralization mechanism that is unexpected because it involves the bacteria excreting proteins for nanoparticle aggregation away from the cells, said Peter Weber, one of the LLNL authors of the paper appearing in the June 15 edition of the journal Science.

Weber and LLNL colleague Ian Hutcheon used LLNLs NanoS
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Contact: Anne Stark
stark8@llnl.gov
925-422-9799
DOE/Lawrence Livermore National Laboratory
14-Jun-2007