Researchers at the University of Illinois at Chicago show how a newly discovered molecular motor helps a cell determine which way is up.

The study was published in the July 31 issue of the Journal of Cell Biology.

The sense of top and bottom is often lost in cells that become cancerous and may be an important factor in metastasis.

Cells depend on the location of a number of proteins and lipids to recognize and maintain their polarity. Moving these lipids and proteins from where they are produced to where they are needed is a dynamic process.

Dr. Athar Chishti, professor of pharmacology at the UIC College of Medicine and principal investigator of the study, said researchers knew that a lipid called PIP3 is very important in signalling polarity. But the process everyone wanted to understand, he said, "was the trafficking and disposition of this lipid to where it was needed at the growing tips of the cells."

Chishti's research team, including Kaori Horiguchi and Dr. Toshihiko Hanada, determined that one domain of a molecular motor protein called a kinesin that they had discovered interacted with PIP3 binding protein. They showed that the kinesin and this binding protein motored the PIP3 along microtubules -- the tracks upon which the kinesins move their cargoes.

They also showed that the kinesin-binding adaptor and PIP3 were found together at the tips of the nerve cells and that in one of these cell types these molecules were most abundant in the longest extension, called the axon.

"We found the motor, the binding domains and an adaptor," said Chishti. "When the adaptor binds PIP3, it is delivered to the membrane where it is needed, and if you block this process, polarity is lost."

In some cells, like neurons, there are dramatic differences in the structure and function of the different ends of a cell. But even in cells where the difference between the ends is not as obvi
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Contact: Jeanne Galatzer-Levy
jgala@uic.edu
312-996-1583
University of Illinois at Chicago
30-Aug-2006