HOME >> BIOLOGY >> NEWS
Mutation in a single gene switches a fungus-grass symbiosis from mutualistic to antagonistic

chlo festucae and its host, perennial ryegrass, Lolium perenne. As a result, they discovered a novel role for reactive oxygen species (ROS) in regulating the mutualistic interaction between E. festucae and its grass host.

Tanaka et al. used a forward genetics approach to create mutants of the endophyte that would be unable to establish or maintain a mutualistic relationship with perennial ryegrass. They inserted foreign DNA randomly into the genome of Epichlo festucae, resulting in a population of fungal strains having disruptions in different genes throughout the fungal genome. From this collection they isolated a mutant that is unable to synchronize its growth with that of the plant host.

Plants infected with the mutant fungus showed stunted growth, premature senescence, and death, whereas those infected with the wild-type fungus exhibited their usual growth pattern. This was accompanied by a dramatic increase in fungal endophyte growth within the plant compared with plants inoculated with wild-type fungus. The fungal hyphae of the wild type fungus showed limited branching and were mostly oriented parallel to the intercellular spaces of the leaf. On the other hand, the hyphae of the mutant fungus showed extensive colonization of the leaf--similar to a pathogenic infection. As a result, the biomass of the mutant fungus increased significantly compared to wild type. Thus a mutualistic interaction became an antagonistic one with the mutation of a single gene.

Tanaka et al. then went on identify and sequence the fungal gene responsible for the mutant phenotype. They determined that the foreign DNA had disrupted a fungal gene, called noxA, which encodes an enzyme that catalyzes the conversion of molecular oxygen to superoxide. The altered symbiotic phenotype is due to a mutation (caused by the insertion of a segment of foreign DNA) in the E. festucae noxA gene.

NADPH oxidase catalyzes the production of ROS or superoxides by tr
'"/>

Contact: Beatrice Grabowski
beatrice@aspb.org
301-251-0560
American Society of Plant Biologists
22-Mar-2006


Page: 1 2 3

Related biology news :

1. Mutation improves memory, may lead to memory-enhancing pill
2. Mutation in HNF4A associated with an increase in birthweight and macrosomia
3. Mutations point the way to new leukemia drugs
4. Mutation in tumor suppressor gene causes pancreatic islet cells to reproduce
5. Mutation in deafness gene can help heal wounds and prevent infection
6. Mutation in blood stem cells provides clues to cancer development
7. Mutation in brain cells of descendants of Abraham Lincoln suggest he suffered from movement disorder
8. Mutations in the BRAF gene predict sensitivity to a novel class of cancer drugs
9. Mutation may raise prostate-cancer risk in African Americans
10. Mutations in NOTCH1 gene cause aortic valve disease
11. Mutation in clams protects against paralytic shellfish poisoning but raises human health risk

Post Your Comments:
(Date:11/18/2014)... , Nov. 18, 2014  The Secure Identity ... Association today jointly announce the formation of The ... of its Identity and Biometric Entry and ... Framework received official support from BORDERPOL, the international ... improve and provide expertise regarding border security, traveler ...
(Date:11/12/2014)... Fla. , Nov. 12, 2014 Crossmatch™, ... today that its U.are.U ® fingerprint readers have ... chain in Central Mexico . The ... 2014 to eliminate payroll issues caused by employees clocking ... installing the U.are.U fingerprint readers, Montparnasse relied on paper ...
(Date:11/7/2014)... November 6, 2014 Leading Biometric companies ... mobile security revolutionizing online transactions.  Companies in focus today are: ... (NYSE: BABA ), Google Inc. (NASDAQ: ... Inc. (NASDAQ: EBAY ) and MasterCard Inc. (NYSE: ... and NXTDW), a biometric authentication company focused on the growing ...
Breaking Biology News(10 mins):Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3Montparnasse Pasteleria Achieves Time and Attendance Transparency with Crossmatch U.are.U Fingerprint Readers 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 2Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 3Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 4Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 5Universal Authentication Methods Provide More Secure Operations for Biometrics Devices - Company Joins FIDO Alliance for Consumer Protection 6
(Date:11/21/2014)... , Nov. 20, 2014 Polaris Group ... dosed in its Phase 1 trial of ADI-PEG ... the treatment of malignant pleural mesothelioma (MPM) and ... conducting clinical trials on ADI-PEG 20, both as ... the treatment of several other indications, including breast ...
(Date:11/18/2014)... 17, 2014 RPS Diagnostics (RPS®) ... – today announces its third annual partnership of ... Get Smart About Antibiotics Week from November 17-23. ... national campaign designed to highlight the coordinated efforts ... non-profit and for-profit partners to provide education about ...
(Date:11/18/2014)... 18, 2014 American Process Inc. ... formed a partnership with researchers at Georgia Institute ... Technology, and the USDA’s Forest Products Laboratory to ... nanocellulose. Nanocellulose is a rapidly emerging high performance ... project is to replace heavy steel structures within ...
(Date:11/18/2014)... PARK, Calif. , Nov. 18, 2014 /PRNewswire/ ... biotechnology company in the emerging field of regenerative ... program, AST-OPC1 (oligodendrocyte progenitor cells) for complete cervical ... one of ten "Top Advanced Therapies Projects to ... are delighted to receive this significant industry honor, ...
Breaking Biology Technology:Polaris Group Announces Treatment of First Patient in Phase 1 Study of ADI-PEG 20 plus Pemetrexed and Cisplatin in Malignant Pleural Mesothelioma And Non-Squamous Non-Small Cell Lung Carcinoma 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 2RPS Again Partners with the CDC in Support of the 2014 Get Smart About Antibiotics Week 3American Process Inc. Announces Partnership to Develop Ultra-Strong, Lightweight Automotive Components Using Nanotechnology from Nature 2American Process Inc. Announces Partnership to Develop Ultra-Strong, Lightweight Automotive Components Using Nanotechnology from Nature 3Asterias Biotherapeutics Named as a "Top Advanced Therapies Projects to Watch" and Will Present at the Therapeutic Area Partnerships Conference 2Asterias Biotherapeutics Named as a "Top Advanced Therapies Projects to Watch" and Will Present at the Therapeutic Area Partnerships Conference 3
Cached News: