HOME >> BIOLOGY >> NEWS
Mutation in a single gene switches a fungus-grass symbiosis from mutualistic to antagonistic

chlo festucae and its host, perennial ryegrass, Lolium perenne. As a result, they discovered a novel role for reactive oxygen species (ROS) in regulating the mutualistic interaction between E. festucae and its grass host.

Tanaka et al. used a forward genetics approach to create mutants of the endophyte that would be unable to establish or maintain a mutualistic relationship with perennial ryegrass. They inserted foreign DNA randomly into the genome of Epichlo festucae, resulting in a population of fungal strains having disruptions in different genes throughout the fungal genome. From this collection they isolated a mutant that is unable to synchronize its growth with that of the plant host.

Plants infected with the mutant fungus showed stunted growth, premature senescence, and death, whereas those infected with the wild-type fungus exhibited their usual growth pattern. This was accompanied by a dramatic increase in fungal endophyte growth within the plant compared with plants inoculated with wild-type fungus. The fungal hyphae of the wild type fungus showed limited branching and were mostly oriented parallel to the intercellular spaces of the leaf. On the other hand, the hyphae of the mutant fungus showed extensive colonization of the leaf--similar to a pathogenic infection. As a result, the biomass of the mutant fungus increased significantly compared to wild type. Thus a mutualistic interaction became an antagonistic one with the mutation of a single gene.

Tanaka et al. then went on identify and sequence the fungal gene responsible for the mutant phenotype. They determined that the foreign DNA had disrupted a fungal gene, called noxA, which encodes an enzyme that catalyzes the conversion of molecular oxygen to superoxide. The altered symbiotic phenotype is due to a mutation (caused by the insertion of a segment of foreign DNA) in the E. festucae noxA gene.

NADPH oxidase catalyzes the production of ROS or superoxides by tr
'"/>

Contact: Beatrice Grabowski
beatrice@aspb.org
301-251-0560
American Society of Plant Biologists
22-Mar-2006


Page: 1 2 3

Related biology news :

1. Mutation improves memory, may lead to memory-enhancing pill
2. Mutation in HNF4A associated with an increase in birthweight and macrosomia
3. Mutations point the way to new leukemia drugs
4. Mutation in tumor suppressor gene causes pancreatic islet cells to reproduce
5. Mutation in deafness gene can help heal wounds and prevent infection
6. Mutation in blood stem cells provides clues to cancer development
7. Mutation in brain cells of descendants of Abraham Lincoln suggest he suffered from movement disorder
8. Mutations in the BRAF gene predict sensitivity to a novel class of cancer drugs
9. Mutation may raise prostate-cancer risk in African Americans
10. Mutations in NOTCH1 gene cause aortic valve disease
11. Mutation in clams protects against paralytic shellfish poisoning but raises human health risk

Post Your Comments:
(Date:7/8/2015)... , July 8, 2015 Summary ... and the fourth most fatal, with a mortality rate ... poor prognosis of pancreatic cancer patients has highlighted a ... which is not being met by the current market. ... array of products with varying molecule types and mechanisms ...
(Date:7/7/2015)... , July 7, 2015  Based on its ... & Sullivan recognizes Credence ID, LLC with the ... Company of the Year Award. Credence ID has ... in its mission of offering enrollment and identification ... As Credence ID was formed by experts from ...
(Date:6/30/2015)... , June 30, 2015 To bolster its ... provider HYPR Corp. announced today the addition of two new ... serve as board advisor and David Raviv will ... team underscore HYPR Corp.,s commitment to providing the most secure ... Dimitri Sirota co-founded Layer 7 Technologies, a provider of ...
Breaking Biology News(10 mins):Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 2Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 3Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 4Frost & Sullivan Acknowledges the Distinctive Customer-focused Attributes of Credence ID's Mobile Biometric Product Suite 5HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 2HYPR Corp. Expands Biometric Authentication Team with Enterprise All Stars 3
(Date:7/30/2015)... VANCOUVER, Washington , July 30, 2015 ... "Biosimulation Market, by Product (Software, Molecular Modeling, PB/PK, PKPD, ... (Drug Discovery & Development, Preclinical, Clinical Trial), by End ... the Biosimulation Market is expected to reach USD 2,107.99 ... at a CAGR of 15.29%. Browse ...
(Date:7/30/2015)... over 60,000 customers across the globe, ISN improves the efficiency and effectiveness of contractor ... 60,000 safe and reliable contractors in over 80 countries. ... ... Over 60,000 Worldwide Customers In More Than ... Headquartered in Dallas, TX , ISN has additional offices in ...
(Date:7/30/2015)... York, NY (PRWEB) , ... July 30, 2015 , ... As part of its 2015 ... raw material and technical service business units, Whitehouse Laboratories is pleased to ... will be adjacent to the Container Center of Excellence and will be strictly dedicated to ...
(Date:7/29/2015)... , July 30, 2015 According ... Market - Growth, Trends & Forecasts (2015-2020), , published by ... US$ 30.25 billion by the end of 2020, with ... accounting for more than 40% of the global market size. ... at a CAGR of 13.7% during the period of (2015-2020). ...
Breaking Biology Technology:Biosimulation Market Worth 2,107.99 Million USD by 2020 2Biosimulation Market Worth 2,107.99 Million USD by 2020 3Biosimulation Market Worth 2,107.99 Million USD by 2020 4ISN Reaches Over 60,000 Worldwide Customers 2ISN Reaches Over 60,000 Worldwide Customers 3Whitehouse Labs Formally Announces Plans to Open a Microbiological Laboratory 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 2Asia Pacific to Dominate the Global Stem Cell Market - Vericel Corporation, Ocata Therpeutics, Athersys Inc., Cryo-Cell International, Geron Corporation, StemCells, Inc. Among the Major Players 3
Cached News: