Michael L. Metzker, Ph.D., Human Genome Sequencing Center, Baylor College of Medicine, Houston
$2 million (3 years)
"Ultrafast SBS (Sequencing by Synthesis) Method for Large-Scale Human Resequencing"
This team will focus on developing novel fluorescent, photolabile nucleotide terminators for sequencing by synthesis, as well as making improvements to enzymes called DNA polymerases that will support their accurate incorporation into DNA. This is part of the group's plan to eventually build a full-scale sequencing system.

Stephen R. Quake, Ph.D., Stanford University, Palo Alto, Calif.
$1.8 million (3 years)
"High-Throughput, Single-Molecule DNA Sequencing"
This group will try to improve its sequencing-by-synthesis technology in order to achieve longer reads from very large numbers of single DNA molecules. The key to the technology's single molecule sensitivity is the detection of fluorescence resonance energy transfer on a total internal reflection microscope.

Mostafa Ronaghi, Ph.D., Stanford Genome Technology Center, Palo Alto, Calif.
$1.8 million (3 years)
"Pyrosequencing Array for DNA Sequencing"
The principal investigator of this team is an inventor of pyrosequencing, which uses unmodified nucleotides and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminescent signal. The group of researchers will work on further developing a highly integrated and parallel format with improved equipment for detection of the chemiluminescent signals resulting in a portable and inexpensive device for low-cost genome sequencing.

Jingyue Ju, Ph.D., Columbia University, New York
$1.8 million (3 years)
"An Integrated System for DNA Sequencing by Synthesis"
One focu
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Contact: Geoff Spencer
spencerg@mail.nih.gov
301-492-0911
NIH/National Human Genome Research Institute
14-Oct-2004