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New grants bolster efforts to generate faster and cheaper tools for DNA sequencing

pproaches have many complementary elements that integrate biochemistry, chemistry and physics with engineering to enhance the whole effort to develop the next generation of DNA sequencing and analysis technologies.

"The different approaches will likely result in several successful and complementary technologies. We will monitor carefully to see how each technology progresses and which of them can ultimately be used by the average researcher or health care provider, said Jeffery Schloss, Ph.D., NHGRI's program director for technology development. "Each research team brings a unique set of skills and expertise to solving difficult scientific and engineering problems."

$1,000 Genome Grants

NHGRIs Revolutionary Genome Sequencing Technologies grants have as their goal the development of breakthrough technologies that will enable a human-sized genome to be sequenced for $1,000 or less. Grant recipients and their approximate total funding are:

  • Richard B. Fair, Ph.D., Duke University, Durham, N.C.
    $3,686,000 (3 years)
    Continuous Sequencing-by-Synthesis, Based on a Digital Microfluidic Platform

This group has already shown the potential of droplet-based microfluidics in sequencing-by-synthesis. Their new goals are to extend read length, minimize reaction volume and increase throughput to 10,000 reactions in a very small area. Separating the chemistry and detection steps makes this technology more efficient, and the droplet-based microfluidics system solves many of the difficulties involved with complicated fluid handling on a very small scale.

  • Stuart Lindsay, Ph.D., Arizona State University, Tempe
    $877,000 (3 years)
    Sequencing by Recognition

A nanometer is one-billionth of a meter, much too small to be seen with a conventional lab microscope. Several groups are developing nanopores (holes about two nanometers in diameter) that may be able to reco
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Contact: Geoff Spencer
spencerg@mail.nih.gov
301-402-0911
NIH/National Human Genome Research Institute
1-Aug-2007


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