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New grants bolster efforts to generate faster and cheaper tools for DNA sequencing

gnize individual DNA bases by their electrical or ionic signals to achieve high-accuracy sequencing of individual DNA molecules. This research team seeks to develop molecular wires that are sufficiently flexible and sensitive to enable this type of sequencing.

  • Xinsheng Sean Ling, Ph.D., Brown University, Providence, R.I.
    $820,000 (3 years)
    Hybridization-Assisted Nanopore DNA Sequencing

Investigating further the potential of nanopore technology, these researchers intend to use solid-state nanopores to detect the location, along a DNA strand, where another short, known DNA sequence attaches by hybridization (base-pairing). By doing this experiment many times with many different short, known sequences, the sequence of long DNA strands would be determined.

  • Wlodek Mandecki, Ph.D., University of Medicine and Dentistry of New Jersey, Newark
    $1,672,000 (3 years)
    Ribosome-Based Single Molecule Method to Acquire Sequence Data from Genomes

This researcher and his team will modify key components of the ribosome the translation system that cells use to build proteins on messenger RNA templates to read out the sequence of nucleotide building blocks along that message. Any DNA molecule can be converted to such a message, so by sequencing the messenger RNA, the sequence of the DNA itself could be determined.

  • Andre Marziali, Ph.D., University of British Columbia, Vancouver
    $746,000 (3 years)
    Nanopore Array Force Spectroscopy Chip for Rapid Clinical Genotyping

These investigators will develop solid-state, nanopore-based force spectroscopy for rapid electronic detection of sequence variation. The project builds on the teams previous demonstration of the ability to detect sequences at single base resolution using organic nanopore force spectroscopy.

  • John S. Oliver, Ph.D., NABsys, Inc., Providence, R.I.
    $498,000 (2 years) '"/>



Contact: Geoff Spencer
spencerg@mail.nih.gov
301-402-0911
NIH/National Human Genome Research Institute
1-Aug-2007


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