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New grants bolster efforts to generate faster and cheaper tools for DNA sequencing

tional grants aim to make improvements that could be implemented in the near future to further improve sequencing at this dramatically-lowered cost. Grant recipients and their approximate total funding are:

  • Jeremy S. Edwards, Ph.D. , University of New Mexico School of Medicine, Albuquerque
    $900,000 (3 years)
    Polony Sequencing the Human Genome

The ultimate goal of this team is to use established polony genome sequencing technology to re-sequence a human genome within a week for less than $10,000. To meet their goal, they will continue improving the quality of sequencing data and further develop the computational tools needed to assemble a human genome sequence.

  • Jingyue Ju, Ph.D., Columbia University, New York (Two Grants)
    $644,000 (2 years)
    3-O-Modified Nucleotide Reversible Terminators for Pyrosequencing

This investigator will design a library of synthetic molecular tools designed to optimize the pyrosequencing method to overcome the difficulties deciphering repetitive regions of DNA templates. The current pyrosequencing method utilizes unmodified DNA base pairs and polymerases to synthesize DNA and a firefly enzyme to generate a chemiluminescent signal.

  • Jingyue Ju, Ph.D., Columbia University, New York
    $2,217,000 (2 years)
    An Integrated System for DNA Sequencing by Synthesis

This team will continue its development and optimization of a novel set of fluorescent nucleotide reversible terminators for sequencing by synthesis. A new method for preparing DNA-beads for attachment to a substrate will also be developed. The goal is to increase the length of the DNA sequencing reads while maintaining high data quality.

  • David C. Schwartz, Ph.D., University of Wisconsin, Madison
    $882,000 (3 years)
    Sequence Acquisition from Mapped Single DNA Molecules

The focus of this project is to create a system
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Contact: Geoff Spencer
spencerg@mail.nih.gov
301-402-0911
NIH/National Human Genome Research Institute
1-Aug-2007


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