HOME >> BIOLOGY >> NEWS
New technique boosts size of proteins that can be analyzed

Imagine you had to break a secret code, but you could see only part of the message. That's the kind of frustration researchers face when trying to identify proteins and characterize how those proteins are modified in cells by biological processes.

But now, Cornell researchers have extended a powerful technique to increase by fourfold the size of a protein that can be analyzed, to those containing more than 2,000 amino acids, up from about 500.

Called a "top-down" approach, the technique uses a mass spectrometer, which measures the masses of ions or charged particles. Researchers break up the protein into pieces and weigh both the masses of the whole protein and of the individual pieces. By matching the weight of the whole protein and its pieces with those of known protein sequences in a database, they can identify the protein. Any differences in mass with known proteins can help researchers also find where and how proteins have been modified in cells.

For example, if a section of a protein has an increased weight of 16, researchers can tell that the protein has been oxidized within that section, which means that an oxygen atom (with an atomic weight of 16) was added.

"When you isolate a protein from a mixture, your first problem is to know which one it is," said Fred McLafferty, Cornell's Peter J.W. Debye Professor Emeritus of Chemistry and Chemical Biology and senior author of the paper published in the Oct. 6 issue of the journal Science. "Mass spectrometry characterizes a protein by measuring the masses produced from it."

The new technique provides a far more efficient way to break down proteins inside the mass spectrometer -- sprayed, heated and then bashed with gas molecules -- to obtain their pieces for the mass analysis. They are sprayed in an electric field where they pick up charges and are vaporized as they pass through a heated capillary. Then they are hit by air molecules in low-energy collisions to keep the protein from foldi
'"/>

Contact: Press Relations Office
pressoffice@cornell.edu
607-255-6074
Cornell University News Service
5-Oct-2006


Page: 1 2

Related biology news :

1. Innovative tagging technique may help researchers better protect fish stocks
2. Innovative research technique reveals another natural wonder in Yellowstone Park
3. New techniques redefine assessment of liver disease
4. New technique will produce a better chromosome map
5. Researchers develop technique for bacteria crowd control
6. Bioluminescence at the service of a novel cerebral imaging technique
7. Natural antibiotics yield secrets to atom-level imaging technique
8. New imaging technique tracks traffic patterns of white blood cells
9. Vet College gets grant to develop fish virus diagnostic technique
10. Harvard team creates spray drying technique for TB vaccine
11. Novel laboratory technique nudges genes into activity

Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/21/2020)... SCOTTSDALE, Ariz. (PRWEB) , ... May 20, 2020 , ... ... justice, and remove danger for all of us. The positions of these men and ... as from the defense for not following rules precisely. Officers have always had to ...
(Date:5/15/2020)... FREDERICK, Md. (PRWEB) , ... May 15, 2020 , ... ... a boutique intellectual property law firm based in Frederick, Maryland and Tysons Corner, Virginia, ... the field of biotechnology and a another in the field of chemistry. The firm ...
(Date:5/14/2020)... ... May 13, 2020 , ... DuPont ... latest enzyme for the lactose-free dairy market. This unique product allows for process ... dairy products at competitive costs across North America. , Among the numerous benefits ...
Breaking Biology News(10 mins):
(Date:7/7/2020)... ... July 06, 2020 , ... Bio-IT World has announced ... Bristol-Myers Squibb, the University of Chicago, Massachusetts General Hospital, Mission: Cure, and the ... awards program, highlighting outstanding examples of how technology innovations and strategic initiatives can ...
(Date:6/28/2020)... (PRWEB) , ... June 25, 2020 , ... The Indoor ... launch of Safe Space™ for businesses of all types to ensure cleanliness, distancing and ... , A Safe Space™ enabled business will provide a health performance index ...
(Date:6/23/2020)... ... June 23, 2020 , ... ... unique lab-made bioresearch materials, today announced the availability of the Delta-G-VSV Pseudotyping System ... University of Tennessee, enables studies of SARS-CoV-2 viral entry and COVID-19 vaccine effectiveness ...
(Date:6/23/2020)... ... June 23, 2020 , ... ... today announced that it has filed an Investigational New Drug application (IND) with ... trial of its lead drug candidate NBTX-001 in patients with panic disorder (PD). ...
Breaking Biology Technology:
Cached News: